Results 61 to 70 of about 4,939,783 (282)

Proteomics Analysis of the Nucleolus in Adenovirus-infected Cells

Molecular & Cellular Proteomics, 2010
Adenoviruses replicate primarily in the host cell nucleus, and it is well established that adenovirus infection affects the structure and function of host cell nucleoli in addition to coding for a number of nucleolar targeted viral proteins. Here we used unbiased proteomics methods, including high throughput mass spectrometry coupled with stable ...
Lam, Yun W., Evans, Vanessa C., Heesom, Kate J., Lamond, Angus I., Matthews, David A.   +4 more
openaire   +3 more sources

SITES OF NUCLEOLUS PRODUCTION IN CULTURED CHINESE HAMSTER CELLS [PDF]

The Journal of Cell Biology, 1969
Chinese hamster cell strains in the early passages in culture display wide variation in number of nucleolus-like bodies per cell, though such strains are characteristically euploid. A variety of criteria indicate that the nucleolus-like bodies are true nucleoli. Their Azure B- and fast green-staining properties indicate the presence of RNA and protein;
S G, Phillips, D M, Phillips
openaire   +2 more sources

Dynamic Regulation of Endogenous Transcription Factor Hubs at Single‐Molecule Resolution

Advanced Science, EarlyView.
This study combines single‐molecule microscopy and genome editing to characterize the dynamic behaviors of endogenous oncofusion transcription factor EWS::FLI1 in Ewing sarcoma cells. EWS::FLI1 forms neomorphic hubs that dynamically assemble and dissolve. The hubs are regulated during mitosis, by RNA, and by specific chemicals.
Shawn Yoshida, Yanghao Zhong, Akiko Kumagai, William G. Dunphy, Shasha Chong   +4 more
wiley   +1 more source

Mechanical Activation of Piezo1 Drives Osteoarthritis Through Kdm5c‐Mediated Epigenetic Silencing

Advanced Science, EarlyView.
Excessive mechanical stress activates Piezo1, triggering Ca2+‐dependent cytoskeletal forces that deform the nucleus and reduce H3K4me3. Kdm5c demethylates H3K4me3 at Col2a1 and Runx3 promoters. Kdm5c knockout rescues degradation. Repurposed telmisartan directly inhibits Kdm5c, blocking this axis and showing disease‐modifying efficacy in mouse OA models
Tianyou Kan, Xuran Li, Han Wang, Lin Sun, Yao Wang, Jiangdong Wu, Junqi Cui, Yiqi Yang, Kai Yuan, Linyang Chu, Liao Wang, Hanjun Li, Mengning Yan, Zhifeng Yu   +13 more
wiley   +1 more source

RNA Pol-II transcripts in nucleolar associated domains of cancer cell nucleoli

Nucleus
We performed a comparative study of the non-ribosomal gene content of nucleoli from seven cancer cell lines, using identical methods of purification and analysis.
Soumya Roy Chowdhury, Arunima Shilpi, Gary Felsenfeld   +2 more
doaj   +1 more source

Myeloma cells contain high levels of inorganic polyphosphate which is associated with nucleolar transcription

Haematologica, 2012
Background In hematology there has recently been increasing interest in inorganic polyphosphate. This polymer accumulates in platelet granules and its functions include modulating various stages of blood coagulation, inducing angiogenesis, and provoking ...
Maria D. Jimenez-Nuñez, David Moreno-Sanchez, Laura Hernandez-Ruiz, Alicia Benítez-Rondán, Ana Ramos-Amaya, Beatriz Rodríguez-Bayona, Francisco Medina, José Antonio Brieva, Felix A. Ruiz   +8 more
doaj   +1 more source

A Bright Ratiometric Dipyrene Probe for Functional Imaging of Condensate Microenvironments in Living Cells

Advanced Science, EarlyView.
Ratiometric dipyrene imaging resolves monomer (M) and excimer (E) channels and computes pixel‐wise E/M maps within biomolecular condensates, yielding physicochemical fingerprints shaped by coupled polarity‐related solvation and microviscosity. These spatial fingerprints enable quantitative phenotyping of cellular states, exemplified by separation of ...
Koki Matsumoto, Sora Kitai, Yoshio Nishiyama, Shogo Amemori, Kei Makiyama, Dini Kurnia Ikliptikawati, Kentaro Ohira, Yuta Kozuka, Maho Tobita, Shih‐Cheng Chen, Chien‐Hung Yu, Wei‐Min Liu, De‐Chen Lin, Motohiro Mizuno, Kazuma Ogawa, Koichi Ogami, Kenji Takahashi, Hiroshi I. Suzuki, Richard W. Wong, Takahiro Soeta, Masaharu Hazawa   +20 more
wiley   +1 more source

Effect of short- and long-lasting chilling on pre-rRNA synthesis and transport in root meristematic cells of three soybean cultivars

Acta Societatis Botanicorum Poloniae, 2011
Autoradiographic studies of 3H-uridine incorporation (20-min incubation) and dynamics of radioactive particle translocation from nucleolus into cytoplasm (following 80-min postincubation in non-radioactive medium) in root meristematic cells of soybean ...
Dariusz Stępiński
doaj   +1 more source

8‐Oxoguanine Modified CircMTUS1 Drives PABPC1 Phase Separation to Promote Gastric Cancer Progression and Cisplatin Resistance via Autophagy

Advanced Science, EarlyView.
ABSTRACT Gastric cancer (GC) is a major global health concern, as its prevention and treatment remain significant challenges. The 8‐oxoguanine (o8G) modification of circRNAs, alongside their capacity to orchestrate liquid‐liquid phase separation (LLPS) and autophagy, plays a pivotal role in driving tumor progression and determining therapeutic outcomes.
Lei Peng, Lurong Li, Zhenghui Zhu, Chenyang Li, Huaiming Sang, Guoxin Zhang, Xuan Li, Yini Dang, Yuanyuan Li   +8 more
wiley   +1 more source

Quantitative nucleolar proteomics reveals nuclear re-organization during stress- induced senescence in mouse fibroblast

BMC Cell Biology, 2011
Background Nucleolus is the most prominent mammalian organelle within the nucleus which is also the site for ribosomal biogenesis. There have been many reports indicating the involvement of nucleolus in the process of aging.
Zhou Zhongjun, Liu Baohua, kar Bishnupriya, Lam Yun W   +3 more
doaj   +1 more source