Results 171 to 180 of about 6,341,201 (297)

Protocol for isolating nuclei from human stem cell-derived grafts for single-nucleus RNA sequencing

Single-nucleus RNA sequencing enables high-resolution transcriptomic profiling of brain tissue, facilitating analysis of cell identity in models of neurodegeneration and repair.
Storm, Petter, Fiorenzano, Alessandro, Sozzi, Edoardo, Storm, Petter,, Sozzi, Edoardo,, Fiorenzano, Alessandro,, Lund University.   +6 more
core   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Fast and tortuous: extreme deformations and fast movements of the cell nucleus are driven by microtubules in the model fungus <i>Podospora anserina</i>. [PDF]

mBio
Viron M-c, Kachaner Z, Grognet P, Lalanne C, Guichet A, Brun S.   +5 more
europepmc   +1 more source

An Integrative Segmentation Framework for Cell Nucleus of Fluorescence Microscopy. [PDF]

Genes (Basel), 2022
Pan W, Liu Z, Song W, Zhen X, Yuan K, Xu F, Lin GN.   +6 more
europepmc   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Cryogenic electron tomography reveals herpesvirus capsid assembly intermediates inside the cell nucleus. [PDF]

Nat Commun
Oliver SL, Chen M, Engel L, Hecksel CW, Zhou X, Schmid MF, Arvin AM, Chiu W.   +7 more
europepmc   +1 more source

Dual supervised sampling networks for real-time segmentation of cervical cell nucleus. [PDF]

Comput Struct Biotechnol J, 2022
Luo D, Kang H, Long J, Zhang J, Chen L, Quan T, Liu X.   +6 more
europepmc   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Exploring Shared Biomarkers of Myocardial Infarction and Alzheimer's Disease via Single-Cell/Nucleus Sequencing and Bioinformatics Analysis. [PDF]

J Alzheimers Dis, 2023
Xue W, He W, Yan M, Zhao H, Pi J.
europepmc   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source