Results 81 to 90 of about 5,980,419 (302)

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes [PDF]

, 2005
Studies of higher-order chromatin arrangements are an essential part of ongoing attempts to explore changes in epigenome structure and their functional implications during development and cell differentiation.
Kreth Gregor, Michael R Speicher, Bolzer Andreas, Cremer Christoph, Kreth, G., Andreas Bolzer (83), Cremer, Christoph, Eils, R., Christoph Cremer, Christoph Cremer (91), Fauth Christine, Irina Solovei, Solovei, Irina, Daniela Koehler, Gregor Kreth (84), Eils, Roland, Gregor Kreth, Kaan Saracoglu, Bolzer, Andreas, Koehler Daniela, Irina Solovei (85), Fauth, C., Thomas Cremer (93), Bolzer, A., Speicher, Michael R., Müller Stefan, Solovei Irina, Müller, Stefan, Saracoglu, Kaan, Stefan Müller, Fauth, Christine, Roland Eils (90), Kreth, Gregor, Cremer Thomas, Koehler, D., Stefan Müller (89), Michael R Speicher (27551), Thomas Cremer, Solovei, I., Roland Eils, Müller, S., Cremer, C., Koehler, Daniela, Speicher, M.R., Eils Roland, Speicher Michael R, Cremer, Thomas, Andreas Bolzer, Daniela Koehler (86), Christine Fauth (88), Cremer, T., Saracoglu Kaan, Christine Fauth, Kaan Saracoglu (87), Saracoglu, K.   +54 more
core   +1 more source

LR White is preferable to Unicryl for immunogold detection of fixationsensitive nuclear antigens

European Journal of Histochemistry, 2010
The purpose of this study was to compare two electron microscopy embedding media – LR White and Unicryl – with regard to cell morphologyical and immunohistochemical preservation properties for the study of fixation-sensitive nuclear antigens.
V. V. Philimonenko, J. Janácek, P. Hozák   +2 more
doaj   +1 more source

Subcellular Location of Tirapazamine Reduction Dramatically Affects Aerobic but Not Anoxic Cytotoxicity

Molecules, 2020
Hypoxia is an adverse prognostic feature of solid cancers that may be overcome with hypoxia-activated prodrugs (HAPs). Tirapazamine (TPZ) is a HAP which has undergone extensive clinical evaluation in this context and stimulated development of optimized ...
Chris P. Guise, Maria R. Abbattista, Robert F. Anderson, Dan Li, Rana Taghipouran, Angela Tsai, Su Jung Lee, Jeff B. Smaill, William A. Denny, Michael P. Hay, William R. Wilson, Kevin O. Hicks, Adam V. Patterson   +12 more
doaj   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Posthatching development of synapses in the neuropil of nucleus rotundus of the zebra finch: a quantitative electron microscopic study [PDF]

, 1986
Nixdorf B, Bischof H-J. Posthatching development of synapses in the neuropil of nucleus rotundus of the zebra finch: a quantitative electron microscopic study. The Journal of Comparative Neurology.
Nixdorf, Barbara, Barbara Nixdorf, Hans‐Joachim Bischof, Bischof, Hans-Joachim   +3 more
core   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

The Cdc42 effectors Ste20, Cla4, and Skm1 down-regulate the expression of genes involved in sterol uptake by a mitogen-activated protein kinase-independent pathway [PDF]

, 2009
© 2009 by The American Society for Cell Biology. Under the License and Publishing Agreement, authors grant to the general public, effective two months after publication of (i.e.,.
Jacquier, N, Höfken, T, Lin, Meng, Jacquier, Nicolas, Schneiter, R, Unden, H, Just, Ursula, Just, U, Unden, Heike, Höfken, Thomas, Schneiter, Roger   +10 more
core   +1 more source

Orderly mitosis shapes interphase genome architecture

eLife
Genomes assume a complex 3D architecture in the interphase cell nucleus. Yet the molecular mechanisms that determine global genome architecture are only poorly understood.
Krishnendu Guin, Adib Keikhosravi, Raj Chari, Gianluca Pegoraro, Tom Misteli   +4 more
doaj   +1 more source

Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry

FEBS Letters, EarlyView.
EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri, Ayaka Nagasato‐Ichikawa, Keita Iida, Shuhei Kimura, Akira Imamoto, Mariko Okada   +5 more
wiley   +1 more source