CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly [PDF]
Journal of Cell Science, 2015 ABSTRACT Although it is generally accepted that chromatin containing the histone H3 variant CENP-A is an epigenetic mark maintaining centromere identity, the pathways leading to the formation and maintenance of centromere chromatin remain unclear.
N. Shono +8 more
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CENP-C/H/I/K/M/T/W/N/L and hMis12 but not CENP-S/X participate in complex formation in the nucleoplasm of living human interphase cells outside centromeres. [PDF]
PLoS ONE, 2018 Kinetochore proteins assemble onto centromeric chromatin and regulate DNA segregation during cell division. The inner kinetochore proteins bind centromeres while most outer kinetochore proteins assemble at centromeres during mitosis, connecting the ...
Christian Hoischen +3 more
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CENP-A K124 Ubiquitylation Is Required for CENP-A Deposition at the Centromere [PDF]
Developmental Cell, 2015 CENP-A is a centromere-specific histone H3 variant that epigenetically determines centromere identity to ensure kinetochore assembly and proper chromosome segregation, but the precise mechanism of its specific localization within centromeric heterochromatin remains obscure.
Niikura, Yohei +5 more
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Step-wise assembly, maturation and dynamic behavior of the human CENP-P/O/R/Q/U kinetochore sub-complex. [PDF]
PLoS ONE, 2012 Kinetochores are multi-protein megadalton assemblies that are required for attachment of microtubules to centromeres and, in turn, the segregation of chromosomes in mitosis. Kinetochore assembly is a cell cycle regulated multi-step process.
Anja Eskat +14 more
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CENP-C reshapes and stabilizes CENP-A nucleosomes at the centromere [PDF]
Science, 2015 Building stable centromeres Each of our chromosomes has a single centromere, seen as a constriction during cell division, which is required for accurate chromosome segregation to daughter cells. Falk et al. show that the special histone protein known as CENP-A, which forms part of the nucleosomes at ...
S. J. Falk +11 more
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CENP-A/CENP-B uncoupling in the evolutionary reshuffling of centromeres in equids
Genome BiologyBackground While CENP-A is the epigenetic determinant of the centromeric function, the role of CENP-B, a centromeric protein binding a specific DNA sequence, the CENP-B-box, remains elusive. In the few mammalian species analyzed so far, the CENP-B box is
Eleonora Cappelletti +5 more
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Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N [PDF]
Nature Cell Biology, 2009 Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity.
Carroll, Christopher W. +4 more
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Chromosome congression by CENP-E and CENP-Q dependent pathways [PDF]
The timely and efficient movement of chromosomes to the spindle equator during mitosis is a prerequisite for accurate chromosome segregation. Recent work has shown that the majority of chromosomes are able to congress and biorientate almost instantaneously after nuclear envelope breakdown due to their position relative to the forming spindle.
Bancroft, James M.
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Phosphorylation of CENP-A on serine 7 does not control centromere function [PDF]
Nature Communications, 2019 Phosphorylation of CENP-A on serine 7 has been proposed to control centromere assembly and function. Here, the authors use gene targeting at both endogenous CENP-A alleles and gene replacement in human cells to demonstrate that CENP-A that cannot be ...
Viviana Barra +9 more
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Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, and CENP-T [PDF]
Proceedings of the National Academy of Sciences, 2017 Significance The centromere is a highly specialized genomic locus playing a critical role in chromosome segregation during cell division and is made of characteristic repetitive DNA sequences. Due to their repetitive nature, it has been difficult to study the organization of human centromeres.
Giunta S., Funabiki H.
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