Re-examining the role of Drosophila Sas-4 in centrosome assembly using two-colour-3D-SIM FRAP
eLife, 2015 Centrosomes have many important functions and comprise a ‘mother’ and ‘daughter’ centriole surrounded by pericentriolar material (PCM). The mother centriole recruits and organises the PCM and templates the formation of the daughter centriole. It has been
Paul T Conduit +4 more
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Dissecting the Structural Organization, Recruitment and Activation Mechanisms of Centrosomal γ‐TuRCs
Cytoskeleton, EarlyView.ABSTRACT Visualizing human centrosomes using cryo‐electron tomography revealed the native structure and molecular organization of γ‐tubulin ring complexes (γ‐TuRCs). γ‐TuRCs localized to two distinct centrosomal pools, one in the pericentriolar material (PCM) and another in the centriole lumen, which is released during mitosis.
Florian W. Hofer +5 more
wiley +1 more source
Ofd1, a human disease gene, regulates the length and distal structure of centrioles [PDF]
, 2010 SUMMARYCentrosomes and their component centrioles represent the principal microtubule organizing centers of animal cells. Here we show that the gene underlying Orofaciodigital Syndrome 1, Ofd1, is a component of the distal centriole that controls ...
García Verdugo, José Manuel +3 more
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LZTS2 Negatively Regulates Centrosomal CEP135 Levels and Microtubule Nucleation
Cytoskeleton, EarlyView.ABSTRACT The microtubule cytoskeleton is a fundamental functional component of the cell. In vertebrate proliferating cells, centrosomes are the primary microtubule organizing center (MTOC), and their dysregulation has been linked to genomic instability and cancer.
Catarina Peneda +4 more
wiley +1 more source
Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells
eLifeSAS-6 (SASS6) is essential for centriole formation in human cells and other organisms but its functions in the mouse are unclear. Here, we report that Sass6-mutant mouse embryos lack centrioles, activate the mitotic surveillance cell death pathway, and ...
Marta Grzonka, Hisham Bazzi
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A novel atypical sperm centriole is functional during human fertilization
Nature Communications, 2018 The two zygote centrioles are paternally inherited; however, their development is incompletely understood. Here, the authors show that the distal centriole is remodeled into an atypical centriole which functions as the zygote’s second centriole.
Emily L. Fishman +12 more
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Centriole Assembly Requires Both Centriolar and Pericentriolar Material Proteins [PDF]
, 2004 Centrioles organize pericentriolar material to form centrosomes and also template the formation of cilia. Despite the importance of centrioles in dividing and differentiated cells, their assembly remains poorly understood at a molecular level.
Dammermann, Alexander +5 more
core +1 more source
Dynamics of primary cilia in endothelial and mesenchymal cells throughout mouse lung development
Developmental Dynamics, EarlyView.Abstract Cilia are specialized structures found on a variety of mammalian cells, with variable roles in the transduction of mechanical and biological signals (by primary cilia, PC), as well as in the generation of fluid flow (by motile cilia). Their critical role in the establishment of a left–right axis in early development is well described, as well ...
Stephen Spurgin +8 more
wiley +1 more source
Self-organization of Plk4 regulates symmetry breaking in centriole duplication
Nature Communications, 2019 During centriole duplication, Plk4 regulates formation of a single daughter centriole adjacent to the mother centriole, but the mechanism is unclear. Here, the authors show that Plk4 can undergo liquid–liquid phase separation and that the condensation ...
Shohei Yamamoto, Daiju Kitagawa
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New phagotrophic euglenoid species (new genus Decastava; Scytomonas saepesedens; Entosiphon oblongum), Hsp90 introns, and putative euglenoid Hsp90 pre-mRNA insertional editing [PDF]
, 2015 We describe three new phagotrophic euglenoid species by light microscopy and 18S rDNA and Hsp90 sequencing: Scytomonas saepesedens; Decastava edaphica; Entosiphon oblongum. We studied Scytomonas and Decastava ultrastructure.
Cavalier-Smith, Thomaa +2 more
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