Results 81 to 90 of about 20,027 (280)

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source

Self-organization of Plk4 regulates symmetry breaking in centriole duplication

Nature Communications, 2019
During centriole duplication, Plk4 regulates formation of a single daughter centriole adjacent to the mother centriole, but the mechanism is unclear. Here, the authors show that Plk4 can undergo liquid–liquid phase separation and that the condensation ...
Shohei Yamamoto, Daiju Kitagawa
doaj   +1 more source

Centriolar SAS-7 acts upstream of SPD-2 to regulate centriole assembly and pericentriolar material formation

eLife, 2017
The centriole/basal body is a eukaryotic organelle that plays essential roles in cell division and signaling. Among five known core centriole proteins, SPD-2/Cep192 is the first recruited to the site of daughter centriole formation and regulates the ...
Kenji Sugioka, Danielle R Hamill, Joshua B Lowry, Marie E McNeely, Molly Enrick, Alyssa C Richter, Lauren E Kiebler, James R Priess, Bruce Bowerman   +8 more
doaj   +1 more source

Mechanisms of HsSAS-6 assembly promoting centriole formation in human cells [PDF]

, 2014
SAS-6 proteins are thought to impart the ninefold symmetry of centrioles, but the mechanisms by which their assembly occurs within cells remain elusive. In this paper, we provide evidence that the N-terminal, coiled-coil, and C-terminal domains of HsSAS ...
Arquint, Azimzadeh, Azimzadeh, Beck, Bettencourt-Dias, Bornens, Bulseco, Carvalho-Santos, Cavalier-Smith, Chen, Chrétien, Cizmecioglu, Debora Keller, Dickson, Dippell, Dzhindzhev, Gopalakrishnan, Guichard, Guichard, Gönczy, Hatch, Haupts, Hoh, Jan Ellenberg, Kim, Kitagawa, Kleylein-Sohn, Kuriyama, Lau, Lawo, Leidel, Lettman, Lin, Lukinavičius, Maeder, Malte Wachsmuth, Marshall, Mennella, Meritxell Orpinell, Middendorp, Nakazawa, Nicolas Olivier, Nigg, Olivier, Olivier, Pierre Gönczy, Puklowski, Robert Mahen, Romain Wyss, Schmidt, Sonnen, Stevens, Strnad, Strnad, Strnad, Suliana Manley, van Breugel, Virginie Hachet, Vladar, Vulprecht, Wachsmuth   +60 more
core   +3 more sources

Cytotoxicity of Atropisomeric [1,1′‐Binaphthalene]‐2,2′‐Diamines (BINAM) and Analogs in Human Cancer Cells: Enantioselectivity, Structure–Activity Relationships, and Mechanism

ChemMedChem, EarlyView.
It is reported for the first time that atropisomeric (R)‐[1,1′‐binaphthalene]‐2,2′‐diamine (R‐BINAM, 1(R)), but not (S)‐[1,1′‐binaphthalene]‐2,2′‐diamine (S‐BINAM, 1(S)), is a moderately potent spindle poison, causing antiproliferation, depolymerization of microtubules, multipolar spindles, pericentriolar material (PCM) fragmentation, mitotic ...
Malte Eichelbaum, Patrick J. Bednarski
wiley   +1 more source

Dynamics of primary cilia in endothelial and mesenchymal cells throughout mouse lung development

Developmental Dynamics, EarlyView.
Abstract Cilia are specialized structures found on a variety of mammalian cells, with variable roles in the transduction of mechanical and biological signals (by primary cilia, PC), as well as in the generation of fluid flow (by motile cilia). Their critical role in the establishment of a left–right axis in early development is well described, as well ...
Stephen Spurgin, Ange Michelle Nguimtsop, Fatima N. Chaudhry, Sylvia N. Michki, Jocelynda Salvador, M. Luisa Iruela‐Arispe, Jarod A. Zepp, Saikat Mukhopadhyay, Ondine Cleaver   +8 more
wiley   +1 more source

Cell division: The renaissance of the centriole [PDF]

Current Biology, 1999
Centrioles are located at the center of the cytoskeleton and duplicate exactly once per cell cycle. Recent studies suggest that centrioles are required for the organization of a functional centrosome and that centriole assembly requires both gamma- and delta-tubulin.
Wallace F. Marshall, Joel L. Rosenbaum
openaire   +2 more sources

A novel atypical sperm centriole is functional during human fertilization

Nature Communications, 2018
The two zygote centrioles are paternally inherited; however, their development is incompletely understood. Here, the authors show that the distal centriole is remodeled into an atypical centriole which functions as the zygote’s second centriole.
Emily L. Fishman, Kyoung Jo, Quynh P. H. Nguyen, Dong Kong, Rachel Royfman, Anthony R. Cekic, Sushil Khanal, Ann L. Miller, Calvin Simerly, Gerald Schatten, Jadranka Loncarek, Vito Mennella, Tomer Avidor-Reiss   +12 more
doaj   +1 more source

Patterns of selection against centrosome amplification in human cell lines.

PLoS Computational Biology, 2021
The presence of extra centrioles, termed centrosome amplification, is a hallmark of cancer. The distribution of centriole numbers within a cancer cell population appears to be at an equilibrium maintained by centriole overproduction and selection ...
Marco António Dias Louro, Mónica Bettencourt-Dias, Claudia Bank   +2 more
doaj   +1 more source

Augmin-dependent microtubule nucleation at microtubule walls in the spindle [PDF]

, 2013
© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Cell Biology 202 (2013): 25-33, doi:10.1083/jcb.201304031.The formation of a functional ...
Goshima, Gohta, Kamasaki, Tomoko, Kita, Shigeo, McIntosh, J. Richard, Osumi, Masako, O’Toole, Eileen, Usukura, Jiro   +6 more
core   +1 more source