Results 81 to 90 of about 45,269 (354)

Cell-free protein synthesis with technical additives – expanding the parameter space of in vitro gene expression

Beilstein Journal of Organic Chemistry
Biocatalysis has established itself as a successful tool in organic synthesis. A particularly fast technique for screening enzymes is the in vitro expression or cell-free protein synthesis (CFPS).
Tabea Bartsch, Stephan Lütz, Katrin Rosenthal   +2 more
doaj   +1 more source

Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response

Protein & Cell, 2020
The cyclic GMP-AMP (cGAMP) synthase (cGAS) plays a critical role in host defense by sensing cytosolic DNA derived from microbial pathogens or mis-located cellular DNA. Upon DNA binding, cGAS utilizes GTP and ATP as substrates to synthesize cGAMP, leading
Mi Li, Hong-Bing Shu
doaj   +1 more source

Overlapping Patterns of Rapid Evolution in the Nucleic Acid Sensors cGAS and OAS1 Suggest a Common Mechanism of Pathogen Antagonism and Escape [PDF]

, 2015
A diverse subset of pattern recognition receptors (PRRs) detects pathogen-associated nucleic acids to initiate crucial innate immune responses in host organisms.
Clark, NL, Elde, NC, Hagan, C, Hancks, DC, Hartley, MK   +4 more
core   +4 more sources

Pre‐Encoded IFN‐I Sensitivity Exacerbates Memory T Cell Senescence in Solid Tumors

Advanced Science, EarlyView.
Type I interferon (IFN‐I) signaling promotes p21‐dependent cell cycle arrest in senescent tumor‐specific memory T cells, resulting in poor proliferative responses and solid tumor regression during cancer vaccination. Conversely, IFNα/β receptor blockade reinvigorates T cell proliferation to regress solid tumors and is more effective with increasing ...
Andrew Nguyen, Scott R Walsh, Li Deng, Lan Chen, Yonghong Wan   +4 more
wiley   +1 more source

Interferon‐Driven Biomarkers and Synergistic Therapy for PRMT5 Inhibition in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
Triple‐negative breast cancer exhibits variable sensitivity to PRMT5 inhibition. Basal interferon signaling is identified as a key biomarker of response. PARP inhibition with olaparib induces IFN signaling, sensitizing resistant TNBC cells to PRMT5 inhibitors.
Ziwen Zhang, Sheyu Zhang, Lu Guo, Yichun Pan, Juan Huang, Yishuai Ji, Jiaqi Tao, Yong Wei, Xiaojia Wang, Qin Wu   +9 more
wiley   +1 more source

Phase Diagrams for Sonoluminescing Bubbles [PDF]

, 1996
Sound driven gas bubbles in water can emit light pulses. This phenomenon is called sonoluminescence (SL). Two different phases of single bubble SL have been proposed: diffusively stable and diffusively unstable SL.
Birkhoff G., Guderley G., Plesset M. S., Prosperetti A., Strube H. W.   +4 more
core   +4 more sources

Chikungunya virus antagonizes cGAS-STING mediated type-I interferon responses by degrading cGAS

PLOS Pathogens, 2020
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus known to cause epidemics resulting in predominantly symptomatic infections, which in rare cases cause long term debilitating arthritis and arthralgia. Significant progress has been made in understanding the roles of canonical RNA sensing pathways in the host recognition of CHIKV; however, less is
L. G. Webb, J. Veloz, J. Pintado-Silva, T. Zhu, M. V. Rangel, T. Mutetwa, L. Zhang, D. Bernal-Rubio, D. Figueroa, L. Carrau, R. Fenutria, U. Potla, St. P. Reid, J. S. Yount, K. A. Stapleford, S. Aguirre, A. Fernandez-Sesma   +16 more
openaire   +4 more sources

Tailoring Vascular‐Immune Homeostasis via Manganese‐DNA Complex‐Armed Immunogenic Extracellular Vesicles for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang, Lihuan Shang, Xiaochun Chen, Xiangzhan Kong, Xiaojuan Wang, Linjia Jiang, Huiying Zhao, Yafeng Zhu, Cheng Huang, Shiyi Deng, Rui Zhang, Minghui Wang, Haoming Lin, Ping‐Pui Wong   +13 more
wiley   +1 more source

T cell-intrinsic cGAS-CTCF regulation maintains regulatory T cell development and function

Cell Reports
Summary: While cGAS is known as a cytosolic DNA sensor in innate immunity, its function in T cells and adaptive immunity remains uncharacterized. This study investigates the role of cGAS in regulatory T (Treg) cells.
Hongpeng Li, Yuchen Zhang, Shangze Gao, Hang Yin   +3 more
doaj   +1 more source

Streptavidin Promotes DNA Binding and Activation of cGAS to Enhance Innate Immunity

iScience, 2020
Summary: cGAS/STING signaling plays an essential role in sensing cytosolic DNA. cGAS activity is regulated by posttranslational modifications and binding partners. cGAS interactome largely includes mammalian or viral proteins.
Yanqiong Zhang, Zhe Ma, Ying Wang, Joshua Boyer, Guoxin Ni, Liang Cheng, Siyuan Su, Zhigang Zhang, Zhichuan Zhu, Jiayi Qian, Lishan Su, Qi Zhang, Blossom Damania, Pengda Liu   +13 more
doaj   +1 more source