Results 21 to 30 of about 311,648 (345)

Pathogen Resistance Depending on Jacalin-Dirigent Chimeric Proteins Is Common among Poaceae but Absent in the Dicot Arabidopsis as Evidenced by Analysis of Homologous Single-Domain Proteins

Plants, 2022
MonocotJRLs are Poaceae-specific two-domain proteins that consist of a jacalin-related lectin (JRL) and a dirigent (DIR) domain which participate in multiple developmental processes, including disease resistance.
Lara Esch, Christian Kirsch, Lara Vogel, Jana Kelm, Nikolai Huwa, Maike Schmitz, Thomas Classen, Ulrich Schaffrath   +7 more
doaj   +1 more source

Expression and purification of toxic anti-breast cancer p28-NRC chimeric protein

Advanced Biomedical Research, 2016
Background: Chimeric proteins consisting of a targeting moiety and a cytotoxic moiety are now under intense research focus for targeted therapy of cancer.
Meysam Soleimani, Hamid Mirmohammad-Sadeghi, Hojjat Sadeghi-Aliabadi, Ali Jahanian-Najafabadi   +3 more
doaj   +1 more source

Recombinant Fusion Protein Joining E Protein Domain III of Tick-Borne Encephalitis Virus and HSP70 of Yersinia pseudotuberculosis as an Antigen for the TI-Complexes

Biomolecules, 2018
Domain III (DIII) of the tick-borne encephalitis virus (TBEV) protein E contains epitopes, which induce antibodies capable of neutralizing the virus. To enhance the immunogenicity of this protein, which has a low molecular weight, the aim of the present ...
Vasily Golotin, Nina Sanina, Ludmila Davydova, Natalia Chopenko, Andrey Mazeika, Manuel Roig, Valery Shnyrov, Vladimir N. Uversky, Eduard Kostetsky   +8 more
doaj   +1 more source

Lineage A betacoronavirus NS2 proteins and the homologous torovirus Berne pp1a carboxy-terminal domain are phosphodiesterases that antagonize activation of RNase L [PDF]

, 2017
Viruses in the family Coronaviridae, within the order Nidovirales, are etiologic agents of a range of human and animal diseases, including both mild and severe respiratory diseases in humans.
Chen, Annie I, Elliott, Ruth, Goldstein, Stephen A, Jha, Babal K, Li, Yize, Quiroz-Figueroa, Katherine, Silverman, Robert H, Thornbrough, Joshua M, Weiss, Susan R, Zhang, Rong   +9 more
core   +2 more sources

Engineering E. coli cell surface in order to develop a one-step purification method for recombinant proteins

AMB Express, 2018
Sortases are enzymes mostly found in Gram-positive bacteria which cleave proteins site-specifically. This feature makes them a promising tool in molecular biology and biotechnology.
Hamidreza Fasehee, Amin Rostami, Fatemeh Ramezani, Gholamreza Ahmadian   +3 more
doaj   +1 more source

N-terminus of Flagellin Fused to an Antigen Improves Vaccine Efficacy against Pasteurella Multocida Infection in Chickens

Vaccines, 2020
Flagellin from bacteria elicits a proinflammatory immune response and may act as a vaccine adjuvant. In this study, we evaluated the adjuvant effect of the N-terminus of flagellin (residues 1–99) when linked to an antigen (a truncated, conserved domain ...
Thu-Dung Doan, Hsian-Yu Wang, Guan-Ming Ke, Li-Ting Cheng   +3 more
doaj   +1 more source

Different domains cooperate to target the human ribosomal L7a protein to the nucleus and to the nucleoli. [PDF]

, 1997
The human ribosomal protein L7a is a component of the major ribosomal subunit. We transiently expressed in HeLa cells L7a-β-galactosidase fusion proteins and studied their subcellular localization by indirect immunofluorescence staining with anti-β ...
Giulia RUSSO, PIETROPAOLO C., RICCIARDELLI G.   +2 more
core   +1 more source

Expression of a Bifunctional Chimeric Protein A-Vargula hilgendorfii Luciferase in Mammalian Cells

BioTechniques, 1996
We have designed and constructed a novel chimeric protein that consisted of a single domain of protein A and luciferase derived from sea-firefly Vargula hilgendorfii with the goal of obtaining a heterofunctional immunological tool. The structural gene of
Y. Maeda, H. Ueda, T. Hara, J. Kazami, G. Kawano, E. Suzuki, T. Nagamune   +6 more
doaj   +1 more source

Designing a P48-40 Chimeric Protein of Mycoplasma agalactiae and Highly Expression in E. coli, Applicable for Indirect ELISA [PDF]

Brazilian Archives of Biology and Technology, 2023
Mycoplasma agalactiae is an economically important pathogen and the main etiological agent of contagious agalactiae (CA) in small ruminants with a relatively high prevalence in sheep and goat, mainly characterized by mastitis, conjunctivitis and ...
Shayesteh Shookouh-Zangeneh, Majid Esmaelizad, Aylar Safari, Nazanin Esmaelizad   +3 more
doaj   +1 more source

Efficient processing of an antigenic sequence for presentation by MHC class I molecules depends on its neighboring residues in the protein [PDF]

, 1991
Processing of endogenously synthesized proteins generates short peptides that are presented by MHC class I molecules to CD8 T lymphocytes. Here it is documented that not only the sequence of the presented peptide but also the residues by which it is ...
Braciale, Chimini, Colbere-Garapin, Del Val, Del Val, Del Val, DeLisi, Falk, Falk, Ganem, Hans-Jürgen Schlicht, Jonjić, Junker, Keil, Margarita Del Val, Matthias J. Reddehase, Nassal, Ou, Parham, Ponta, Reddehase, Reddehase, Rothbard, Rötzschke, Rötzschke, Schlicht, Schlicht, Thomas Ruppert, Townsend, Townsend, Townsend, Townsend, Townsend, Ulrich H. Koszinowski, Van Bleek, Volkmer, Yewdell   +36 more
core   +1 more source