Borrelia burgdorferi BmpA-BBK32 and BmpA-BBA64: New Recombinant Chimeric Proteins with Potential Diagnostic Value [PDF]
Pathogens, 2021 Currently, the diagnosis of Lyme disease is based mostly on two-tiered serologic testing. In the new generation of immunoenzymatic assays, antigens comprise whole-cell lysates of members of the Borrelia burgdorferi sensu lato (s.l.) species complex, with
Weronika Grąźlewska +3 more
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Evaluation of chimeric proteins for serological diagnosis of brucellosis in cattle [PDF]
Veterinary World, 2021 Background and Aim: An accurate diagnosis of Brucella-infected animals is one of the critical measures in eradication programs. Conventional serological tests based on whole-cell (WC) antigens and detecting antibodies against pathogen-associated ...
Aitbay K. Bulashev +3 more
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Stability Assessment of Four Chimeric Proteins for Human Chagas Disease Immunodiagnosis [PDF]
Biosensors, 2021 The performance of an immunoassay relies on antigen-antibody interaction; hence, antigen chemical stability and structural integrity are paramount for an efficient assay.
Paola Alejandra Fiorani Celedon +9 more
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Protlego: a Python package for the analysis and design of chimeric proteins. [PDF]
Bioinformatics, 2021 Abstract Motivation Duplication and recombination of protein fragments have led to the highly diverse protein space that we observe today. By mimicking this natural process, the design of protein chimeras via fragment recombination has proven experimentally successful and has opened a new era ...
Ferruz N, Noske J, Höcker B.
europepmc +5 more sources
A review of chimeric proteins/enzymes [PDF]
BIO Web of ConferencesThe low homologous protein chimeric recombination technique by rational design of mutant library proteins in the construction of chimeric esterases by structural domain recombination often produces a large number of inclusion bodies due to misfolding of ...
Zhu Jiakang, Han Song, Gao Le
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No more than three PlpE non-overlapping epitopes trigger significant antibody production in individuals vaccinated with the Pasteurella multocida epitope-chimeric proteins [PDF]
Microbiology SpectrumCurrent vaccine research still confronts multiple challenges, and epitope-focused vaccine design serves as an effective technical approach to address these issues.
Binbin Geng +4 more
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The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera. [PDF]
PLoS ONE, 2019 This study presents an evaluation of four tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, GRA1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in ...
Bartłomiej Tomasz Ferra +6 more
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Hybrid Gene Origination Creates Human-Virus Chimeric Proteins during Infection
Cell, 2020 Jessica Sook Yuin Ho +2 more
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Characterization of the Chimeric PriB-SSBc Protein [PDF]
International Journal of Molecular Sciences, 2021 PriB is a primosomal protein required for the replication fork restart in bacteria. Although PriB shares structural similarity with SSB, they bind ssDNA differently. SSB consists of an N-terminal ssDNA-binding/oligomerization domain (SSBn) and a flexible C-terminal protein–protein interaction domain (SSBc).
En-Shyh Lin +2 more
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Frontiers in Immunology, 2022 IntroductionThe anti-CD20 antibody rituximab (RTX) has substantially improved outcomes of patients with B-cell lymphomas, although more efficient therapies are needed for refractory or relapsing lymphomas.
Ana Victoria Casadesús +16 more
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