Results 21 to 30 of about 5,931 (188)
TOP1 CAD-seq: A protocol to map catalytically engaged topoisomerase 1 in human cells
STAR Protocols, 2022 Summary: TOP1 CAD-seq enables mapping of TOP1 sites of covalent engagement with DNA. The procedure depends upon enrichment of DNA-covalent adducts using chaotropic salts and immunoprecipitation with an antibody specific for TOP1. Here, we describe a step-
Vladislav Kuzin +3 more
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STAR Protocols, 2021 Summary: In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally.
Ariane Lismer +4 more
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CSSQ: a ChIP-seq signal quantifier pipeline
Frontiers in Cell and Developmental Biology, 2023 Chromatin immunoprecipitation followed by sequencing (ChIP-seq) has revolutionized the studies of epigenomes and the massive increase in ChIP-seq datasets calls for robust and user-friendly computational tools for quantitative ChIP-seq. Quantitative ChIP-
Ashwath Kumar +5 more
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Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification [PDF]
, 2016 The photoreceptor UV RESISTANCE LOCUS 8 (UVR8) specifically mediates photomorphogenic responses to UV-B wavelengths. UVR8 acts by regulating transcription of a set of genes, but the underlying mechanisms are unknown. Previous research indicated that UVR8
Herzyk, Pawel +2 more
core +1 more source
Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis. [PDF]
, 2019 The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression.
Chory, Joanne +9 more
core +2 more sources
The regulatory and transcriptional landscape associated with carbon utilization in a filamentous fungus. [PDF]
, 2020 Filamentous fungi, such as Neurospora crassa, are very efficient in deconstructing plant biomass by the secretion of an arsenal of plant cell wall-degrading enzymes, by remodeling metabolism to accommodate production of secreted enzymes, and by enabling ...
Benz, J Philipp +15 more
core +3 more sources
Frontiers in Pharmacology, 2023 Curcuphenol, a common component of the culinary spices, naturally found in marine invertebrates and plants, has been identified as a novel candidate for reversing immune escape by restoring expression of the antigen presentation machinery (APM) in ...
Samantha L. S. Ellis +41 more
doaj +1 more source
Nature Communications, 2017 Polycomb repressive complexes modify histones but it is unclear how changes in chromatin states alter kinetics of transcription. Here, the authors use single-cell RNAseq and ChIPseq to find that actively transcribed genes with Polycomb marks have greater
Gozde Kar +9 more
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eLife, 2022 Estrogen (E2) and Progesterone (Pg), via their specific receptors (ERalpha and PR), are major determinants in the development and progression of endometrial carcinomas, However, their precise mechanism of action and the role of other transcription ...
Alejandro La Greca +15 more
doaj +1 more source
RUbioSeq+: A multiplatform application that executes parallelized pipelines to analyse next-generation sequencing data [PDF]
, 2017 This is the peer reviewed version of the following article: Computer Methods and Programs in Biomedine 138 (2016): 73-81, which has been published in final form at http://dx.doi.org/10.1016/j.cmpb.2016.10.008Background and objective To facilitate routine
López-Fernández, Hugo +1 more
core +1 more source