Results 71 to 80 of about 3,719 (149)
The FEBS Journal, Volume 292, Issue 16, Page 4333-4352, August 2025.Under normoxic conditions, the SUMOylated fraction of TFAP2A cannot interact with the Ku70/Ku80 complex, nor it can bind to chromatin. The unSUMOylated fraction pre‐occupies the chromatin of a subset of hypoxia‐induced genes together with the Ku70/Ku80 complex for basal euchromatin formation. Under hypoxic conditions, the SUMOylation equilibrium shifts
Amalia Kanoura +8 more
wiley +1 more source
Efficient yeast ChIP-Seq using multiplex short-read DNA sequencing
BMC Genomics, 2009 Background Short-read high-throughput DNA sequencing technologies provide new tools to answer biological questions. However, high cost and low throughput limit their widespread use, particularly in organisms with smaller genomes such as S.
Yellman Christopher M +7 more
doaj +1 more source
Limitations and possibilities of low cell number ChIP-seq
BMC Genomics, 2012 Background Chromatin immunoprecipitation coupled with high-throughput DNA sequencing (ChIP-seq) offers high resolution, genome-wide analysis of DNA-protein interactions. However, current standard methods require abundant starting material in the range of
Gilfillan Gregor D +8 more
doaj +1 more source
Heat*seq: compare your HTS expreiment with RNAseq/ChIPseq/CAGE public datasets
, 2016 With the establishment of better protocols and decreasing costs, high-throughput sequencing experiments such as RNA-seq or ChIP-seq are now accessible even to small experimental laboratories. However, comparing one or few experiments generated by an individual lab to the vast amount of relevant data available in public domain might be hindered due to ...
openaire +1 more source
Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application
BMC Genomics, 2017 Background Chromatin immunoprecipitation-sequencing (ChIP-seq) is a widely used epigenetic approach for investigating genome-wide protein-DNA interactions in cells and tissues. The approach has been relatively well established but several key steps still
Jian Zhong +9 more
doaj +1 more source
PeakRanger: A cloud-enabled peak caller for ChIP-seq data
BMC Bioinformatics, 2011 Background Chromatin immunoprecipitation (ChIP), coupled with massively parallel short-read sequencing (seq) is used to probe chromatin dynamics. Although there are many algorithms to call peaks from ChIP-seq datasets, most are tuned either to handle ...
Grossman Robert, Feng Xin, Stein Lincoln
doaj +1 more source
The Transcription Factor NFATc1 Supports the Rejection of Heterotopic Heart Allografts
Frontiers in Immunology, 2018 The immune suppressants cyclosporin A (CsA) and tacrolimus (FK506) are used worldwide in transplantation medicine to suppress graft rejection. Both CsA and FK506 inhibit the phosphatase calcineurin (CN) whose activity controls the immune receptor ...
Johannes Baur +12 more
doaj +1 more source
Analysis of a collection of ChIPseq experiments with the ChromHMM program
, 2017 El volumen de información NGS que está disponible para la comunidad científica para estudiar la regulación de genes está en constante crecimiento, por lo que el análisis de estos datos más complejos. Aquí se exploró el uso de la segmentación de la cromatina Métodos, como ChromHMM, para resolver el problema en escenarios particulares. Así, se obtuvieron
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STAR ProtocolsSummary: Angiogenesis begins as endothelial cells migrate, forming a sprouting tip and subsequent growth-rich stalk cells. Here, we present a protocol for transcriptomic and epigenomic analyses of tip-like cells in cultured endothelial cells. We describe
Shintaro Funasaki +9 more
doaj +1 more source