Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling [PDF]
, 2009 Whole mount mitotic metaphase chromosomes of different plants and animals were investigated with high resolution field emission scanning electron microscopy (FESEM) to study the ultrastructural organization of centromeres, including metacentric ...
Schroeder-Reiter, E., Wanner, Gerhard
core +1 more source
Pathways and Mechanisms that Prevent Genome Instability in Saccharomyces cerevisiae. [PDF]
, 2017 Genome rearrangements result in mutations that underlie many human diseases, and ongoing genome instability likely contributes to the development of many cancers.
Kolodner, Richard D +1 more
core +1 more source
Epigenetics and airways disease
Respiratory Research, 2006 Epigenetics is the term used to describe heritable changes in gene expression that are not coded in the DNA sequence itself but by post-translational modifications in DNA and histone proteins. These modifications include histone acetylation, methylation,
Ito Kazuhiro +3 more
doaj +1 more source
Non‐histone Chromosomal Proteins [PDF]
European Journal of Biochemistry, 1975 We describe a method for fractionation of chromatin components by selective dissociation with salt in buffers containing 5 M urea in combination with chromatography on hydroxyapatite at 4 °C. This results in two histone and four non‐histone fractions which are recovered in high yield and with minimal proteolytic contamination.Template capacity ...
Blüthmann, H., Mrozek, S., Gierer, A.
openaire +3 more sources
Transcription as a Threat to Genome Integrity [PDF]
, 2016 Genomes undergo different types of sporadic alterations, including DNA damage, point mutations, and genome rearrangements, that constitute the basis for evolution.
Aguilera López, Andrés +1 more
core +1 more source
HMGA2, the architectural transcription factor high mobility group, is expressed in the developing and mature mouse cochlea. [PDF]
PLoS ONE, 2014 Hmga2 protein belongs to the non-histone chromosomal high-mobility group (HMG) protein family. HMG proteins have been shown to function as architectural transcription regulators, facilitating enhanceosome formation on a variety of mammalian promoters ...
Ibtihel Smeti +4 more
doaj +1 more source
Recent advances in malaria genomics and epigenomics [PDF]
, 2016 Malaria continues to impose a significant disease burden on low- and middle-income countries in the tropics. However, revolutionary progress over the last 3 years in nucleic acid sequencing, reverse genetics, and post-genome analyses has generated step ...
Kirchner, Sebastian +2 more
core +1 more source
Characterizing the DNA Damage Response by Cell Tracking Algorithms and Cell Features Classification Using High-Content Time-Lapse Analysis. [PDF]
PLoS ONE, 2015 Traditionally, the kinetics of DNA repair have been estimated using immunocytochemistry by labeling proteins involved in the DNA damage response (DDR) with fluorescent markers in a fixed cell assay.
Walter Georgescu +6 more
doaj +1 more source
Interaction of Non-histone Chromosomal Proteins HMG1 and HMG2 with DNA [PDF]
European Journal of Biochemistry, 1977 Interaction between non-histone protein HMG1 or HMG2 and DNA has been studied by using thermal denaturation and circular dichroism (CD) spectroscopy. We have made the following observations. 1. The binding of each of these two proteins to DNA stabilizes the latter, as shown by an increase in melting temperature of 20 degrees C (from 45 degrees C to ...
S S, Yu +3 more
openaire +2 more sources
The inner centromere is a biomolecular condensate scaffolded by the chromosomal passenger complex. [PDF]
, 2019 The inner centromere is a region on every mitotic chromosome that enables specific biochemical reactions that underlie properties, such as the maintenance of cohesion, the regulation of kinetochores and the assembly of specialized chromatin, that can ...
A Chakraborty +57 more
core +2 more sources