Results 111 to 120 of about 1,413 (169)
Impact of ultrasonic and heat treatments on the physicochemical properties and rennet-induced coagulation characteristics of milk from various species. [PDF]
Ultrason SonochemHou W +9 more
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On the Question of the Identity of Pepsin and Chymosin
Journal of Biological Chemistry, 1908 openaire +1 more source
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Improved Production of Chymosin in Aspergillus by Expression as a Glucoamylase-Chymosin Fusion
Nature Biotechnology, 1990We have extended the work on chymosin production in Aspergillus by constructing an expression vector in which the cDNA encoding bovine prochymosin B was fused in frame immediately following the codon for the last amino acid of the A. awamori glucoamylase (glaA) gene. Transformation of A.
M, Ward +4 more
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Increased production of chymosin by glycosylation
Journal of Biotechnology, 2006Filamentous fungi are well known in the industry as producers of large amounts of extracellular proteins. However, production levels of heterologous proteins are often disappointing low. In this paper it is shown that increasing glycosylation is a powerful strategy for increasing production levels of chymosin in filamentous fungi.
Hans J M, van den Brink +4 more
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The crystallography of calf rennin (chymosin)
Proceedings of the Royal Society of London. Series B. Biological Sciences, 1971Abstract The growth of crystals of calf rennin (chymosin, † EC 3.4.4.3) and the control of nucleation to produce crystals of desired size, are described. Only one stable species, orthorhombic rennin I, has been found, but a metastable monoclinic species, rennin II, appeared on one occasion in a solution nearly saturated with glycine ...
C W, Bunn, P C, Moews, M E, Baumber
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Journal of Food Processing and Preservation, 1991
The effects of ultrasound treatment on the properties of chymosin and the ultrastructure of abomasum during chymosin extraction were investigated. Ultrasound treatment at 36 W/cm2 for 80 min did not decrease chymosin activity and its proteolytic activity.
S. M. KIM, J. F. ZAYAS
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The effects of ultrasound treatment on the properties of chymosin and the ultrastructure of abomasum during chymosin extraction were investigated. Ultrasound treatment at 36 W/cm2 for 80 min did not decrease chymosin activity and its proteolytic activity.
S. M. KIM, J. F. ZAYAS
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Molecular Biotechnology, 2016
Codon optimization of the Bos taurus Chymosin gene (CYM) for its expression in Pichia pastoris was performed in this study. A synthetic CYM gene was designed in silico by replacing codons rarely used by P. pastoris with equivalent nucleotide combinations that codify for the same amino acid but that are more frequently encountered in the genome of P ...
Jose Alberto, Espinoza-Molina +4 more
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Codon optimization of the Bos taurus Chymosin gene (CYM) for its expression in Pichia pastoris was performed in this study. A synthetic CYM gene was designed in silico by replacing codons rarely used by P. pastoris with equivalent nucleotide combinations that codify for the same amino acid but that are more frequently encountered in the genome of P ...
Jose Alberto, Espinoza-Molina +4 more
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Commercial Levels of Chymosin Production by Aspergillus
Bio/Technology, 1991We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular ...
Nigel S. Dunn-Coleman +15 more
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