Results 141 to 150 of about 3,188 (202)
Some of the next articles are maybe not open access.
Commercial Levels of Chymosin Production by Aspergillus
Bio/Technology, 1991We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular ...
Nigel S. Dunn-Coleman +15 more
openaire +2 more sources
Molecular Biotechnology, 2016
Codon optimization of the Bos taurus Chymosin gene (CYM) for its expression in Pichia pastoris was performed in this study. A synthetic CYM gene was designed in silico by replacing codons rarely used by P. pastoris with equivalent nucleotide combinations that codify for the same amino acid but that are more frequently encountered in the genome of P ...
Jose Alberto, Espinoza-Molina +4 more
openaire +2 more sources
Codon optimization of the Bos taurus Chymosin gene (CYM) for its expression in Pichia pastoris was performed in this study. A synthetic CYM gene was designed in silico by replacing codons rarely used by P. pastoris with equivalent nucleotide combinations that codify for the same amino acid but that are more frequently encountered in the genome of P ...
Jose Alberto, Espinoza-Molina +4 more
openaire +2 more sources
Food Chemistry, 2018
Binary blends of S. marianum-flower extract and chymosin, as coagulant preparations, enabled the manufacture of miniature cheeses with distinctive characteristics compared to those of chymosin-renneted cheeses.
María Laura Colombo +2 more
exaly +2 more sources
Binary blends of S. marianum-flower extract and chymosin, as coagulant preparations, enabled the manufacture of miniature cheeses with distinctive characteristics compared to those of chymosin-renneted cheeses.
María Laura Colombo +2 more
exaly +2 more sources
Applied Microbiology and Biotechnology, 2002
We studied the effect of coexpression of chymosin and chymosin-binding llama single-domain antibody fragments (VHHs) on the secretion of chymosin by Saccharomyces cerevisiae cells. A VHH expression library containing chymosin-specific VHHs was obtained by immunization of a llama and coexpressed with chymosin in yeast. From this library, we obtained two
Harmsen, M.M., Smits, C.B., de Geus, B.
openaire +2 more sources
We studied the effect of coexpression of chymosin and chymosin-binding llama single-domain antibody fragments (VHHs) on the secretion of chymosin by Saccharomyces cerevisiae cells. A VHH expression library containing chymosin-specific VHHs was obtained by immunization of a llama and coexpressed with chymosin in yeast. From this library, we obtained two
Harmsen, M.M., Smits, C.B., de Geus, B.
openaire +2 more sources
Assessing the authenticity of animal rennet using δ15N analysis of chymosin.
Food Chemistry, 2019Chymosin is a protease that curdles the milk casein. Animal rennet was the first discovered source of chymosin and its use is mandatory for the production of PDO cheeses such as Parmigiano Reggiano and Grana Padano.
F. Camin +6 more
semanticscholar +1 more source
Chymosin-catalyzed peptide synthesis.
Biomedica biochimica acta, 1992Calf chymosin catalyzes peptide synthesis optimally at pH 4-5 giving satisfactory yields of methyl esters or p-nitroanilides of benzyloxycarbonyl tetra- to hexapeptides, provided that hydrophobic amino acid residues form the new peptide bond. The enzyme efficiency depends also on the nature of adjacent amino acid residues.
C A, Abdel Malak, G I, Lavrenova
openaire +1 more source
[Biospecific chromatography of chymosin].
Biokhimiia (Moscow, Russia), 1976Chromatography of commercial rennet was studied on biospecific sorbents obtained by means of coupling of activated Sepharose 4B with epsilon-aminocapronyl-D-phenylalanine methyl ester and amide, epsilon-aminocapronyl-L-phenylalanyl-D-phenylalanine methyl ester, gramicidin S and N-2,4-dinitrophenylhexamethylenediamine.
V M, Stepanov +4 more
openaire +1 more source
Journal of Food Processing and Preservation, 1991
The effects of ultrasound treatment on the properties of chymosin and the ultrastructure of abomasum during chymosin extraction were investigated. Ultrasound treatment at 36 W/cm2 for 80 min did not decrease chymosin activity and its proteolytic activity.
S. M. KIM, J. F. ZAYAS
openaire +1 more source
The effects of ultrasound treatment on the properties of chymosin and the ultrastructure of abomasum during chymosin extraction were investigated. Ultrasound treatment at 36 W/cm2 for 80 min did not decrease chymosin activity and its proteolytic activity.
S. M. KIM, J. F. ZAYAS
openaire +1 more source
The Presence of Chymosin Inhibitor in Seminal Plasma
Zentralblatt für Veterinärmedizin Reihe A, 1983SummaryDetermination of chymosin, an acid protease, as assessed by the turbidimetric method and by the glycomacropeptide release method, showed that cattle seminal plasma inhibits this enzyme's action on milk and on K‐casein. Fractionation of the proteins present in the seminal plasma by gel filtration on Sephadex G‐10 resulted in its resolution into ...
P, Palta, N C, Ganguli
openaire +2 more sources