Results 1 to 10 of about 1,786,363 (358)
A programmable pAgo nuclease with high activity and specificity for efficient DNA and RNA manipulation [PDF]
Communications BiologyProkaryotic Argonaute proteins (pAgos) are programmable nucleases with potential for the development of novel nucleic acid manipulation tools. Previous studies have shown that several pAgos exhibit strong preferences for either DNA or RNA targets, and ...
Jiao Hu +13 more
doaj +2 more sources
Nucleic Acids Res., 2021 Echinobase (www.echinobase.org) is a third generation web resource supporting genomic research on echinoderms. The new version was built by cloning the mature Xenopus model organism knowledgebase, Xenbase, refactoring data ingestion pipelines and ...
Bradley I. Arshinoff +16 more
semanticscholar +1 more source
An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
Microbiology Spectrum, 2022 DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia
Yuqing Yang +6 more
doaj +1 more source
Simple Growth Complementation Assay in Yeast
Bio-Protocol, 2023 The study of genes and their products is an essential prerequisite for fundamental research. Characterization can be achieved by analyzing mutants or overexpression lines or by studying the localization and substrate specificities of the resulting ...
Robert Fuhrmeister, Jana Streubel
doaj +1 more source
Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains
Microorganisms, 2023 Komagataella phaffii (Pichia pastoris) is a widely known microbial host for recombinant protein production and an emerging model organism in fundamental research. The development of new materials and techniques on this yeast improves heterologous protein
Anastasiya Makeeva +7 more
doaj +1 more source
Applied Sciences, 2023 Swine is a common model organism for biomedical research. Epigenetic reprogramming in somatic cell nuclear transfer (SCNT) embryos does not fully recapitulate the natural DNA demethylation events at fertilisation.
Maciej Grzybek +7 more
doaj +1 more source
Implication of therapeutic cloning for organ transplantation [PDF]
European Journal of Cardio-Thoracic Surgery, 2004 Replacement of damaged myocardium with electrically functional, contracting syncytium with a balanced blood supply remains a key goal for the treatment of hearts damaged by coronary heart disease or other disorders. Stem cell therapy offers a potential solution.
J HESCHELER +9 more
openaire +3 more sources
Primary fibroblast cell cycle synchronization and effects on handmade cloned (HMC) bovine embryos
Ciência Animal Brasileira, 2018 Spatial and temporal synchrony and compatibility between the receptor oocyte and the donor cell nucleus are necessary for the process of embryo cloning to allow nuclear reprogramming and early embryonic development. The objective of the present study was
Natalia Andrea Gómez +2 more
doaj +1 more source
An organism arises from every nucleus.
Folia Histochemica et Cytobiologica, 2009 The fact that, cloning using somatic cell nuclear transfer (SCNT) method has been performed, opened new horizons for cloning, and changed the way of our understanding and approach to cell and nucleus.
Nurullah Keklikoglu
doaj +1 more source
Blasticidin S Deaminase: A New Efficient Selectable Marker for Chlamydomonas reinhardtii
Frontiers in Plant Science, 2020 Chlamydomonas reinhardtii is a model unicellular organism for basic or biotechnological research, such as the production of high-value molecules or biofuels thanks to its photosynthetic ability.
Félix de Carpentier +7 more
doaj +1 more source