Results 1 to 10 of about 1,642,375 (296)

New PALM-compatible integration vectors for use in the Gram-positive model bacterium Bacillus subtilis [PDF]

Microbiology Spectrum
Improvements in super-resolution and single-molecule techniques, along with the development of new fluorescent proteins and labeling methods, have allowed super-resolution imaging of bacterial cells.
Ipek Altinoglu, Rut Carballido-Lopez
doaj   +2 more sources

Echinobase: leveraging an extant model organism database to build a knowledgebase supporting research on the genomics and biology of echinoderms

Nucleic Acids Res., 2021
Echinobase (www.echinobase.org) is a third generation web resource supporting genomic research on echinoderms. The new version was built by cloning the mature Xenopus model organism knowledgebase, Xenbase, refactoring data ingestion pipelines and ...
Bradley I. Arshinoff, G. Cary, K. Karimi, Saoirse Foley, S. Agalakov, Francisco Delgado, Vaneet Lotay, Carolyn Ku, Troy J. Pells, Thomas R. Beatman, Eugene Kim, R. Cameron, P. Vize, C. Telmer, Jenifer C. Croce, C. Ettensohn, V. Hinman   +16 more
semanticscholar   +1 more source

An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency

Microbiology Spectrum, 2022
DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia
Yuqing Yang, Menghui Liu, Tianqi Wang, Qian Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia   +6 more
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Implication of therapeutic cloning for organ transplantation [PDF]

European Journal of Cardio-Thoracic Surgery, 2004
Replacement of damaged myocardium with electrically functional, contracting syncytium with a balanced blood supply remains a key goal for the treatment of hearts damaged by coronary heart disease or other disorders. Stem cell therapy offers a potential solution.
F. Dönmez, Axel Raible, Heribert Bohlen, Helmut Acker, Bernd K. Fleischmann, Philipp Sasse, Juergen Hescheler, F. C. Ling, Heinrich Sauer, Zhongju Lu, Ying Xia, B. K. W. Roell, Wilhelm Bloch, A. Welz, Maria Wartenberg, Eugen Kolossov, Daniela Wenzel, Shohreh Kazemi   +17 more
openaire   +3 more sources

Purification and Cloning of Lipase from Micro-Organism [PDF]

Journal of Current Veterinary Research, 2020
Lipases are a class of serine hydrolase, which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol.
Atef Mohamed, Hanan Hassan, Heba Elgendy
openaire   +2 more sources

Simple Growth Complementation Assay in Yeast

Bio-Protocol, 2023
The study of genes and their products is an essential prerequisite for fundamental research. Characterization can be achieved by analyzing mutants or overexpression lines or by studying the localization and substrate specificities of the resulting ...
Robert Fuhrmeister, Jana Streubel
doaj   +1 more source

Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains

Microorganisms, 2023
Komagataella phaffii (Pichia pastoris) is a widely known microbial host for recombinant protein production and an emerging model organism in fundamental research. The development of new materials and techniques on this yeast improves heterologous protein
Anastasiya Makeeva, Dmitry Muzaev, Maria Shubert, Tatiana Ianshina, Anton Sidorin, Elena Sambuk, Andrey Rumyantsev, Marina Padkina   +7 more
doaj   +1 more source

Cloning, Genomic Organization, and Characterization of a Human Cholinephosphotransferase [PDF]

Journal of Biological Chemistry, 2000
A cholinephosphotransferase activity catalyzes the final step in the de novo synthesis of phosphatidylcholine via the transfer of a phosphocholine moiety from CDP choline to diacylglycerol. Ethanolaminephosphotransferase activity catalyzes a similar reaction substituting CDP ethanolamine as the phosphobase donor.
Graeme Wistow, Christopher R. McMaster, Annette L. Henneberry   +2 more
openaire   +3 more sources

tRNA genes in mycobacteria: organization and molecular cloning [PDF]

Journal of Bacteriology, 1990
DNAs from nine mycobacteria cleaved with restriction endonucleases were hybridized with cDNA probes synthesized to tRNAs from Mycobacterium tuberculosis and Mycobacterium smegmatis. The tRNA genes are conserved, but their gross genomic organization has diverged in six of the nine species examined. Organisms of the M.
Jaya Sivaswami Tyagi, Anil K. Tyagi, Bhargava S   +2 more
openaire   +3 more sources

Methylation Genome-Wide Profiling in Lowly and Highly Efficient Somatic Cell Nuclear Transfer in Pigs

Applied Sciences, 2023
Swine is a common model organism for biomedical research. Epigenetic reprogramming in somatic cell nuclear transfer (SCNT) embryos does not fully recapitulate the natural DNA demethylation events at fertilisation.
Maciej Grzybek, Krzysztof Flisikowski, Tom Giles, Marta Dyjak, Rafal Ploski, Piotr Gasperowicz, Richard D. Emes, Pawel Lisowski   +7 more
doaj   +1 more source