Results 1 to 10 of about 112,137 (215)
New PALM-compatible integration vectors for use in the Gram-positive model bacterium Bacillus subtilis [PDF]
Microbiology SpectrumImprovements in super-resolution and single-molecule techniques, along with the development of new fluorescent proteins and labeling methods, have allowed super-resolution imaging of bacterial cells.
Ipek Altinoglu, Rut Carballido-Lopez
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Advanced Protocol for Molecular Characterization of Viral Genome in Fission Yeast (Schizosaccharomyces pombe) [PDF]
PathogensFission yeast, a single-cell eukaryotic organism, shares many fundamental cellular processes with higher eukaryotes, including gene transcription and regulation, cell cycle regulation, vesicular transport and membrane trafficking, and cell death ...
Jiantao Zhang +3 more
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Detecting a network of hijacked journals by its archive [PDF]
Scientometrics 126, (2021), 2021 This study describes a method to detect hijacked journals based on the analysis of the archives of clone journals. This approach is most effective in discovering a network of hijacked journals that have the same organizer(s). Analysis of the archives of clone journals allowed to detect 62 URLs of hijacked journals.
arxiv +1 more source
An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
Microbiology Spectrum, 2022 DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia
Yuqing Yang +6 more
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Simple Growth Complementation Assay in Yeast
Bio-Protocol, 2023 The study of genes and their products is an essential prerequisite for fundamental research. Characterization can be achieved by analyzing mutants or overexpression lines or by studying the localization and substrate specificities of the resulting ...
Robert Fuhrmeister, Jana Streubel
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Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains
Microorganisms, 2023 Komagataella phaffii (Pichia pastoris) is a widely known microbial host for recombinant protein production and an emerging model organism in fundamental research. The development of new materials and techniques on this yeast improves heterologous protein
Anastasiya Makeeva +7 more
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Applied Sciences, 2023 Swine is a common model organism for biomedical research. Epigenetic reprogramming in somatic cell nuclear transfer (SCNT) embryos does not fully recapitulate the natural DNA demethylation events at fertilisation.
Maciej Grzybek +7 more
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Early life imprints the hierarchy of T cell clone sizes [PDF]
eLife 2020 9:e61639, 2020 The adaptive immune system responds to pathogens by selecting clones of cells with specific receptors. While clonal selection in response to particular antigens has been studied in detail, it is unknown how a lifetime of exposures to many antigens collectively shape the immune repertoire.
arxiv +1 more source
Primary fibroblast cell cycle synchronization and effects on handmade cloned (HMC) bovine embryos
Ciência Animal Brasileira, 2018 Spatial and temporal synchrony and compatibility between the receptor oocyte and the donor cell nucleus are necessary for the process of embryo cloning to allow nuclear reprogramming and early embryonic development. The objective of the present study was
Natalia Andrea Gómez +2 more
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An organism arises from every nucleus.
Folia Histochemica et Cytobiologica, 2009 The fact that, cloning using somatic cell nuclear transfer (SCNT) method has been performed, opened new horizons for cloning, and changed the way of our understanding and approach to cell and nucleus.
Nurullah Keklikoglu
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