Results 1 to 10 of about 110,567 (266)
New PALM-compatible integration vectors for use in the Gram-positive model bacterium Bacillus subtilis. [PDF]
Microbiol SpectrImprovements in super-resolution and single-molecule techniques, along with the development of new fluorescent proteins and labeling methods, have allowed super-resolution imaging of bacterial cells.
Altinoglu I, Carballido-Lopez R.
europepmc +2 more sources
Advanced Protocol for Molecular Characterization of Viral Genome in Fission Yeast (<i>Schizosaccharomyces pombe</i>). [PDF]
PathogensFission yeast, a single-cell eukaryotic organism, shares many fundamental cellular processes with higher eukaryotes, including gene transcription and regulation, cell cycle regulation, vesicular transport and membrane trafficking, and cell death ...
Zhang J, Benko Z, Zhang C, Zhao RY.
europepmc +2 more sources
Detecting a network of hijacked journals by its archive [PDF]
Scientometrics 126, (2021), 2021 This study describes a method to detect hijacked journals based on the analysis of the archives of clone journals. This approach is most effective in discovering a network of hijacked journals that have the same organizer(s). Analysis of the archives of clone journals allowed to detect 62 URLs of hijacked journals.
arxiv +1 more source
An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
Microbiology Spectrum, 2022 DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia
Yuqing Yang +6 more
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Implication of therapeutic cloning for organ transplantation [PDF]
European Journal of Cardio-Thoracic Surgery, 2004 Replacement of damaged myocardium with electrically functional, contracting syncytium with a balanced blood supply remains a key goal for the treatment of hearts damaged by coronary heart disease or other disorders. Stem cell therapy offers a potential solution.
F. Dönmez +17 more
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Purification and Cloning of Lipase from Micro-Organism [PDF]
Journal of Current Veterinary Research, 2020 Lipases are a class of serine hydrolase, which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol.
Atef Mohamed, Hanan Hassan, Heba Elgendy
openaire +2 more sources
Simple Growth Complementation Assay in Yeast
Bio-Protocol, 2023 The study of genes and their products is an essential prerequisite for fundamental research. Characterization can be achieved by analyzing mutants or overexpression lines or by studying the localization and substrate specificities of the resulting ...
Robert Fuhrmeister, Jana Streubel
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Alternative PCR-Based Approaches for Generation of Komagataella phaffii Strains
Microorganisms, 2023 Komagataella phaffii (Pichia pastoris) is a widely known microbial host for recombinant protein production and an emerging model organism in fundamental research. The development of new materials and techniques on this yeast improves heterologous protein
Anastasiya Makeeva +7 more
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Cloning, Genomic Organization, and Characterization of a Human Cholinephosphotransferase [PDF]
Journal of Biological Chemistry, 2000 A cholinephosphotransferase activity catalyzes the final step in the de novo synthesis of phosphatidylcholine via the transfer of a phosphocholine moiety from CDP choline to diacylglycerol. Ethanolaminephosphotransferase activity catalyzes a similar reaction substituting CDP ethanolamine as the phosphobase donor.
Graeme Wistow +2 more
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tRNA genes in mycobacteria: organization and molecular cloning [PDF]
Journal of Bacteriology, 1990 DNAs from nine mycobacteria cleaved with restriction endonucleases were hybridized with cDNA probes synthesized to tRNAs from Mycobacterium tuberculosis and Mycobacterium smegmatis. The tRNA genes are conserved, but their gross genomic organization has diverged in six of the nine species examined. Organisms of the M.
Jaya Sivaswami Tyagi +2 more
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