Results 11 to 20 of about 1,771,671 (365)
An organism arises from every nucleus. [PDF]
Folia Histochemica et Cytobiologica, 2009 The fact that, cloning using somatic cell nuclear transfer (SCNT) method has been performed, opened new horizons for cloning, and changed the way of our understanding and approach to cell and nucleus.
Nurullah Keklikoglu
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High-throughput cloning and expression in recalcitrant bacteria [PDF]
Nature Methods, 2007 We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via ...
Eric R. Geertsma, B. Poolman
semanticscholar +4 more sources
Expression capable library for studies of
BMC Microbiology, 2005 Background The sexually transmitted disease, gonorrhea, is a serious health problem in developed as well as in developing countries, for which treatment continues to be a challenge.
Wachocki Susi +18 more
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New PALM-compatible integration vectors for use in the Gram-positive model bacterium Bacillus subtilis [PDF]
Microbiology SpectrumImprovements in super-resolution and single-molecule techniques, along with the development of new fluorescent proteins and labeling methods, have allowed super-resolution imaging of bacterial cells.
Ipek Altinoglu, Rut Carballido-Lopez
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Advanced Protocol for Molecular Characterization of Viral Genome in Fission Yeast (Schizosaccharomyces pombe) [PDF]
PathogensFission yeast, a single-cell eukaryotic organism, shares many fundamental cellular processes with higher eukaryotes, including gene transcription and regulation, cell cycle regulation, vesicular transport and membrane trafficking, and cell death ...
Jiantao Zhang +3 more
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Nucleic Acids Res., 2021 Echinobase (www.echinobase.org) is a third generation web resource supporting genomic research on echinoderms. The new version was built by cloning the mature Xenopus model organism knowledgebase, Xenbase, refactoring data ingestion pipelines and ...
Bradley I. Arshinoff +16 more
semanticscholar +1 more source
Detecting a network of hijacked journals by its archive [PDF]
Scientometrics 126, (2021), 2021 This study describes a method to detect hijacked journals based on the analysis of the archives of clone journals. This approach is most effective in discovering a network of hijacked journals that have the same organizer(s). Analysis of the archives of clone journals allowed to detect 62 URLs of hijacked journals.
arxiv +1 more source
An Optimized Transformation Protocol for Escherichia coli BW3KD with Supreme DNA Assembly Efficiency
Microbiology Spectrum, 2022 DNA cloning requires two steps: the assembly of recombinant DNA molecules and the transformation of the product into a host organism for replication. High efficiencies in both processes can increase the success rate. Recently, we developed an Escherichia
Yuqing Yang +6 more
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Implication of therapeutic cloning for organ transplantation [PDF]
European Journal of Cardio-Thoracic Surgery, 2004 Replacement of damaged myocardium with electrically functional, contracting syncytium with a balanced blood supply remains a key goal for the treatment of hearts damaged by coronary heart disease or other disorders. Stem cell therapy offers a potential solution.
F. Dönmez +17 more
openaire +3 more sources
Purification and Cloning of Lipase from Micro-Organism [PDF]
Journal of Current Veterinary Research, 2020 Lipases are a class of serine hydrolase, which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol.
Atef Mohamed, Hanan Hassan, Heba Elgendy
openaire +2 more sources