Results 11 to 20 of about 1,642,375 (296)
Primary fibroblast cell cycle synchronization and effects on handmade cloned (HMC) bovine embryos
Ciência Animal Brasileira, 2018 Spatial and temporal synchrony and compatibility between the receptor oocyte and the donor cell nucleus are necessary for the process of embryo cloning to allow nuclear reprogramming and early embryonic development. The objective of the present study was
Natalia Andrea Gómez +2 more
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An organism arises from every nucleus.
Folia Histochemica et Cytobiologica, 2009 The fact that, cloning using somatic cell nuclear transfer (SCNT) method has been performed, opened new horizons for cloning, and changed the way of our understanding and approach to cell and nucleus.
Nurullah Keklikoglu
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Blasticidin S Deaminase: A New Efficient Selectable Marker for Chlamydomonas reinhardtii
Frontiers in Plant Science, 2020 Chlamydomonas reinhardtii is a model unicellular organism for basic or biotechnological research, such as the production of high-value molecules or biofuels thanks to its photosynthetic ability.
Félix de Carpentier +7 more
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bioRxiv, 2019 Over the last decade a new strategy was developed to bypass the difficulties to genetically engineer some microbial species by transferring (or “cloning”) their genome into another organism that is amenable to efficient genetic modifications and ...
E. Ruiz +8 more
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Generating a custom TA-cloning expression plasmid for Lactococcus lactis
BioTechniques, 2012 The growing popularity of the lactic acid bacterium Lactococcus lactis has increased demand for novel high-throughput cloning methods. Here we describe a general TA-cloning methodology and demonstrate its feasibility using the plasmid pNZ8148.
Aleš Berlec, Borut Štrukelj
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Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
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Bioscience, biotechnology and biochemistry, 1999 The fosfomycin resistance gene, fosC, has been cloned from the fosfomycin-producing organism, Pseudomonas syringae PB-5123. Sequence analysis upstream of this gene found a new ORF showing significant homology to 2-hydroxypropylphosphonic acid epoxidase ...
T. Kuzuyama +4 more
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Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe [PDF]
Open Biology, 2015 Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through
Yasutaka Kakui +7 more
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Cloning, organization, and expression of the bioluminescence genes of Xenorhabdus luminescens [PDF]
Journal of Bacteriology, 1990 The lux genes of Xenorhabdus luminescens, a symbiont of the nematode Heterorhabditis bacteriophora, were cloned and expressed in Escherichia coli. The expression of these genes in E. coli was qualitatively similar to their expression in X. luminescens. The organization of the genes is similar to that found in the marine luminous bacteria. Hybridization
M Anhalt +2 more
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Physiology, structure, and regulation of the cloned organic anion transporters [PDF]
Xenobiotica, 2008 1. The transport of negatively charged drugs, xenobiotics, and metabolites by epithelial tissues, particularly the kidney, plays critical roles in controlling their distribution, concentration, and retention in the body. Thus, organic anion transporters (OATs) impact both their therapeutic efficacy and potential toxicity. 2.
John B. Pritchard +2 more
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