Results 11 to 20 of about 1,786,363 (358)
High-throughput cloning and expression in recalcitrant bacteria [PDF]
Nature Methods, 2007 We developed a generic method for high-throughput cloning in bacteria that are less amenable to conventional DNA manipulations. The method involves ligation-independent cloning in an intermediary Escherichia coli vector, which is rapidly converted via ...
Eric R. Geertsma, B. Poolman
semanticscholar +4 more sources
Generating a custom TA-cloning expression plasmid for Lactococcus lactis
BioTechniques, 2012 The growing popularity of the lactic acid bacterium Lactococcus lactis has increased demand for novel high-throughput cloning methods. Here we describe a general TA-cloning methodology and demonstrate its feasibility using the plasmid pNZ8148.
Aleš Berlec, Borut Štrukelj
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Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe [PDF]
Open Biology, 2015 Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through
Yasutaka Kakui +7 more
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Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
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Partial Trajectory: The Story of the Altered Nuclear Transfer-Oocyte Assisted Reprogramming (ANT-OAR) Proposal [PDF]
, 2007 This essay aims to tell the story of the “altered nuclear transfer-oocyte assisted reprogramming,” or ANT-OAR, proposal—from its conception by Professor William Hurlbut of the President’s Council on Bioethics—to its adoption and promotion by a group of ...
Byrnes, W. Malcolm
core +2 more sources
67 ORGAN WEIGHT VARIATION IN CLONED PIGS [PDF]
Reproduction, Fertility and Development, 2004 Previously, we compared adult clones and naturally bred control females using a series of physiological and genetic parameters and found considerable variation in certain traits in clones. In order to more fully understand this clonal variation we examined the organ weight differences among a group of clones from a single litter.
J.A. Piedrahita +2 more
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A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
Microbiology Spectrum, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction.
Ankita J. Sachla +2 more
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bioRxiv, 2019 Over the last decade a new strategy was developed to bypass the difficulties to genetically engineer some microbial species by transferring (or “cloning”) their genome into another organism that is amenable to efficient genetic modifications and ...
E. Ruiz +8 more
semanticscholar +1 more source
Purification and Cloning of Lipase from Micro-Organism [PDF]
Journal of Current Veterinary Research, 2020 Lipases are a class of serine hydrolase, which belongs to the α/β hydrolases super family. Triacylglycerol acyl esters hydrolase are carboxyl esterase that catalyses both hydrolysis and synthesis of ester formed from glycerol. Lipase enzyme that catalyzes the hydrolysis of stored triacylglycerol releasing di and monoglycerol.
Heba Elgendy, Hanan Hassan, Atef Mohamed
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Biotechnological bases of the development of cloned pig embryos
Вавиловский журнал генетики и селекции, 2019 The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov +2 more
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