Results 11 to 20 of about 112,137 (215)
Blasticidin S Deaminase: A New Efficient Selectable Marker for Chlamydomonas reinhardtii
Frontiers in Plant Science, 2020 Chlamydomonas reinhardtii is a model unicellular organism for basic or biotechnological research, such as the production of high-value molecules or biofuels thanks to its photosynthetic ability.
Félix de Carpentier +7 more
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Generating a custom TA-cloning expression plasmid for Lactococcus lactis
BioTechniques, 2012 The growing popularity of the lactic acid bacterium Lactococcus lactis has increased demand for novel high-throughput cloning methods. Here we describe a general TA-cloning methodology and demonstrate its feasibility using the plasmid pNZ8148.
Aleš Berlec, Borut Štrukelj
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Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
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Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe [PDF]
Open Biology, 2015 Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through
Yasutaka Kakui +7 more
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Spread of premalignant mutant clones and cancer initiation in multilayered tissue [PDF]
Annals of Applied Probability 33(1) (2023) 299-343, 2020 Over 80% of human cancers originate from the epithelium, which covers the outer and inner surfaces of organs and blood vessels. In stratified epithelium, the bottom layers are occupied by stem and stem-like cells that continually divide and replenish the upper layers. In this work, we study the spread of premalignant mutant clones and cancer initiation
arxiv +1 more source
Advanced Materials Interfaces, EarlyView., 2023 This review discusses the use of Surface‐Enhanced Raman Spectroscopy (SERS) combined with Artificial Intelligence (AI) for detecting antimicrobial resistance (AMR). Various SERS studies used with AI techniques, including machine learning and deep learning, are analyzed for their advantages and limitations.
Zakarya Al‐Shaebi +4 more
wiley +1 more source
A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
Microbiology Spectrum, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction.
Ankita J. Sachla +2 more
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Characterizing and Evaluating The Impact of Software Interface Clones [PDF]
H. Abdeen, O. Shata, "Characterizing and Evaluating The Impact of
Software Interface Clones", International Journal of Software Engineering &
Applications (IJSEA) 4 (1), Jan. 2013, 2013 Software Interfaces are meant to describe contracts governing interactions between logic modules. Interfaces, if well designed, significantly reduce software complexity and ease maintainability . However, as software evolves, the organization and the quality of software interfaces gradually deteriorate.
arxiv +1 more source
MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus. [PDF]
PLoS ONE, 2008 MicroRNAs play significant roles in development, metabolism and carcinogenesis, however, limited information is available about their primary transcripts and the transcriptional regulation of the microRNA genes.
Guisheng Song, Li Wang
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MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE
Scientific Papers Animal Science and Biotechnologies, 2023 An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL +2 more
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