Results 21 to 30 of about 1,771,572 (266)
Bioscience, biotechnology and biochemistry, 1999 The fosfomycin resistance gene, fosC, has been cloned from the fosfomycin-producing organism, Pseudomonas syringae PB-5123. Sequence analysis upstream of this gene found a new ORF showing significant homology to 2-hydroxypropylphosphonic acid epoxidase ...
T. Kuzuyama +4 more
semanticscholar +1 more source
Module-based construction of plasmids for chromosomal integration of the fission yeast Schizosaccharomyces pombe [PDF]
Open Biology, 2015 Integration of an external gene into a fission yeast chromosome is useful to investigate the effect of the gene product. An easy way to knock-in a gene construct is use of an integration plasmid, which can be targeted and inserted to a chromosome through
Yasutaka Kakui +7 more
doaj +1 more source
Cloning, organization, and expression of the bioluminescence genes of Xenorhabdus luminescens [PDF]
Journal of Bacteriology, 1990 The lux genes of Xenorhabdus luminescens, a symbiont of the nematode Heterorhabditis bacteriophora, were cloned and expressed in Escherichia coli. The expression of these genes in E. coli was qualitatively similar to their expression in X. luminescens. The organization of the genes is similar to that found in the marine luminous bacteria. Hybridization
M Anhalt +2 more
openaire +3 more sources
A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
Microbiology Spectrum, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction.
Ankita J. Sachla +2 more
doaj +1 more source
Spread of premalignant mutant clones and cancer initiation in multilayered tissue [PDF]
Annals of Applied Probability 33(1) (2023) 299-343, 2020 Over 80% of human cancers originate from the epithelium, which covers the outer and inner surfaces of organs and blood vessels. In stratified epithelium, the bottom layers are occupied by stem and stem-like cells that continually divide and replenish the upper layers. In this work, we study the spread of premalignant mutant clones and cancer initiation
arxiv +1 more source
Physiology, structure, and regulation of the cloned organic anion transporters [PDF]
Xenobiotica, 2008 1. The transport of negatively charged drugs, xenobiotics, and metabolites by epithelial tissues, particularly the kidney, plays critical roles in controlling their distribution, concentration, and retention in the body. Thus, organic anion transporters (OATs) impact both their therapeutic efficacy and potential toxicity. 2.
John B. Pritchard +2 more
openaire +3 more sources
Heterologous expression of Cysteine Protease 8 from Trichomonas foetus in Pichia pastoris [PDF]
Journal of Veterinary and Animal Sciences, 2023 Bovine trichomonosis is one of the most neglected venereal diseases of cattle. Trichomonas foetus, the causative organism was known over decades and is responsible for severe reproductive failure.
Karli Geethanjali +2 more
doaj +1 more source
Journal of Microbiology and Biotechnology, 2015 To understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens.
Seo-Hyeon Bang +4 more
semanticscholar +1 more source
MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus. [PDF]
PLoS ONE, 2008 MicroRNAs play significant roles in development, metabolism and carcinogenesis, however, limited information is available about their primary transcripts and the transcriptional regulation of the microRNA genes.
Guisheng Song, Li Wang
doaj +1 more source
Characterizing and Evaluating The Impact of Software Interface Clones [PDF]
H. Abdeen, O. Shata, "Characterizing and Evaluating The Impact of
Software Interface Clones", International Journal of Software Engineering &
Applications (IJSEA) 4 (1), Jan. 2013, 2013 Software Interfaces are meant to describe contracts governing interactions between logic modules. Interfaces, if well designed, significantly reduce software complexity and ease maintainability . However, as software evolves, the organization and the quality of software interfaces gradually deteriorate.
arxiv +1 more source