Results 21 to 30 of about 1,642,375 (296)
A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis
Microbiology Spectrum, 2021 The clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 system from Streptococcus pyogenes has been widely deployed as a tool for bacterial strain construction.
Ankita J. Sachla +2 more
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Journal of Microbiology and Biotechnology, 2015 To understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens.
Seo-Hyeon Bang +4 more
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MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE
Scientific Papers Animal Science and Biotechnologies, 2023 An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL +2 more
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Biotechnological bases of the development of cloned pig embryos
Вавиловский журнал генетики и селекции, 2019 The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov +2 more
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MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus. [PDF]
PLoS ONE, 2008 MicroRNAs play significant roles in development, metabolism and carcinogenesis, however, limited information is available about their primary transcripts and the transcriptional regulation of the microRNA genes.
Guisheng Song, Li Wang
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Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis [PDF]
Journal of Bacteriology, 1990 A cloned chemotaxis operon has been characterized. Thirteen representative che mutations from different complementation groups were localized on the physical map by recombination experiments. The use of integration plasmids established that at least 10 of these complementation groups within this locus are cotranscribed.
M. R. Weinreich +3 more
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The Scientific World Journal, 2003 Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last ...
Claire L. Anderson, Gwyn T. Williams
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High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
Proceedings of the National Academy of Sciences of the United States of America, 1979 A set of vector DNAs (Y vectors) useful for the cloning of DNA fragments in Saccharomyces cerevisiae (yeast) and in Escherichia coli are characterized. With these vectors, three modes of yeast transformation are defined.
K. Struhl +3 more
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Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. [PDF]
Proceedings of the National Academy of Sciences, 1981 The integrated form of simian sarcoma virus (SSV) was molecularly cloned in the Charon 16A strain of bacteriophage lambda. In transfection analysis, the recombinant viral DNAs demonstrated the ability to transform cells in tissue culture at high efficiency.
Sushilkumar G. Devare +2 more
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Molecular Studies on Preproinsulin Gene
MATEC Web of Conferences, 2016 Insulin plays an important role in maintaining the blood glucose level of the body. The β-cells of pancreas produce insulin in the form of precursor that is preproinsulin.
Sabir Sarah, Younas Hooria
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