Results 21 to 30 of about 1,786,363 (358)

MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE

open access: yesScientific Papers Animal Science and Biotechnologies, 2023
An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL   +2 more
doaj  

MiR-433 and miR-127 arise from independent overlapping primary transcripts encoded by the miR-433-127 locus. [PDF]

open access: yesPLoS ONE, 2008
MicroRNAs play significant roles in development, metabolism and carcinogenesis, however, limited information is available about their primary transcripts and the transcriptional regulation of the microRNA genes.
Guisheng Song, Li Wang
doaj   +1 more source

Cloning, Genomic Organization, and Characterization of a Human Cholinephosphotransferase [PDF]

open access: yesJournal of Biological Chemistry, 2000
A cholinephosphotransferase activity catalyzes the final step in the de novo synthesis of phosphatidylcholine via the transfer of a phosphocholine moiety from CDP choline to diacylglycerol. Ethanolaminephosphotransferase activity catalyzes a similar reaction substituting CDP ethanolamine as the phosphobase donor.
A L, Henneberry   +2 more
openaire   +2 more sources

Microarray Assistant Clone Organizer and Array Simulator [PDF]

open access: yesBioTechniques, 2002
Microarrays are extensively used in molecular biology experiments. While several vendors offer microarrays on a variety of platforms, many researchers prefer to use custom microarrays with a selected list of clones for their experiments. Many research centers have established core facilities for the production of custom microarrays.
openaire   +3 more sources

Seabream ghrelin: cDNA cloning, genomic organization and promoter studies [PDF]

open access: yesJournal of Endocrinology, 2006
Recent studies have indicated that ghrelin stimulates growth hormone release from the pituitary via the growth hormone secretagogue receptor (GHSR). We have previously isolated two GHSR subtypes from the pituitary of the black seabream Acanthopagrus schlegeli.
Yeung, Chung M.   +3 more
openaire   +4 more sources

Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E

open access: yesThe Scientific World Journal, 2003
Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last ...
Claire L. Anderson, Gwyn T. Williams
doaj   +1 more source

Cisgenesis: an important sub-invention for traditional plant breeding companies [PDF]

open access: yes, 2009
Modern plant breeding is highly dependent on new technologies to master future problems. More traits have to be combined, frequently originating from wild species. Traditional breeding is connected with linkage drag problems.
Jacobsen, E., Schouten, H.J.
core   +3 more sources

Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. [PDF]

open access: yesProceedings of the National Academy of Sciences, 1981
The integrated form of simian sarcoma virus (SSV) was molecularly cloned in the Charon 16A strain of bacteriophage lambda. In transfection analysis, the recombinant viral DNAs demonstrated the ability to transform cells in tissue culture at high efficiency.
K C, Robbins, S G, Devare, S A, Aaronson
openaire   +2 more sources

Environmental stress responses in Lactococcus lactis [PDF]

open access: yes, 1999
Bacteria can encounter a variety of physical conditions during their life. Bacterial cells are able to survive these (often adverse) conditions by the induction of specific or general protection mechanisms. The lactic acid bacterium Lactococcus lactis is
Kok, Jan,   +2 more
core   +3 more sources

tRNA genes in mycobacteria: organization and molecular cloning

open access: yesJournal of Bacteriology, 1990
DNAs from nine mycobacteria cleaved with restriction endonucleases were hybridized with cDNA probes synthesized to tRNAs from Mycobacterium tuberculosis and Mycobacterium smegmatis. The tRNA genes are conserved, but their gross genomic organization has diverged in six of the nine species examined. Organisms of the M.
S, Bhargava, A K, Tyagi, J S, Tyagi
openaire   +3 more sources

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