Results 31 to 40 of about 1,771,671 (365)
Generating a custom TA-cloning expression plasmid for Lactococcus lactis
BioTechniques, 2012 The growing popularity of the lactic acid bacterium Lactococcus lactis has increased demand for novel high-throughput cloning methods. Here we describe a general TA-cloning methodology and demonstrate its feasibility using the plasmid pNZ8148.
Aleš Berlec, Borut Štrukelj
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Advanced Materials Interfaces, EarlyView., 2023 This review discusses the use of Surface‐Enhanced Raman Spectroscopy (SERS) combined with Artificial Intelligence (AI) for detecting antimicrobial resistance (AMR). Various SERS studies used with AI techniques, including machine learning and deep learning, are analyzed for their advantages and limitations.
Zakarya Al‐Shaebi +4 more
wiley +1 more source
MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE
Scientific Papers Animal Science and Biotechnologies, 2023 An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL +2 more
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Biotechnological bases of the development of cloned pig embryos
Вавиловский журнал генетики и селекции, 2019 The term ‘clone’ in animal biotechnology refers to an organism derived from non-sexual reproduction, which is both a direct offspring and a genetic copy of the parent organism.
A. V. Lopukhov +2 more
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Transcriptional organization of a cloned chemotaxis locus of Bacillus subtilis [PDF]
Journal of Bacteriology, 1990 A cloned chemotaxis operon has been characterized. Thirteen representative che mutations from different complementation groups were localized on the physical map by recombination experiments. The use of integration plasmids established that at least 10 of these complementation groups within this locus are cotranscribed.
M. R. Weinreich +3 more
openaire +3 more sources
Partial Trajectory: The Story of the Altered Nuclear Transfer-Oocyte Assisted Reprogramming (ANT-OAR) Proposal [PDF]
, 2007 This essay aims to tell the story of the “altered nuclear transfer-oocyte assisted reprogramming,” or ANT-OAR, proposal—from its conception by Professor William Hurlbut of the President’s Council on Bioethics—to its adoption and promotion by a group of ...
Byrnes, W. Malcolm
core +2 more sources
The Scientific World Journal, 2003 Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last ...
Claire L. Anderson, Gwyn T. Williams
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Clone size distributions in networks of genetic similarity [PDF]
Physica D, 214, 166-173 (2006), 2006 We build networks of genetic similarity in which the nodes are organisms sampled from biological populations. The procedure is illustrated by constructing networks from genetic data of a marine clonal plant. An important feature in the networks is the presence of clone subgraphs, i.e. sets of organisms with identical genotype forming clones. As a first
arxiv +1 more source
High-frequency transformation of yeast: autonomous replication of hybrid DNA molecules.
Proceedings of the National Academy of Sciences of the United States of America, 1979 A set of vector DNAs (Y vectors) useful for the cloning of DNA fragments in Saccharomyces cerevisiae (yeast) and in Escherichia coli are characterized. With these vectors, three modes of yeast transformation are defined.
K. Struhl +3 more
semanticscholar +1 more source
Fluctuating fitness shapes the clone size distribution of immune repertoires [PDF]
PNAS 113 (2) 274-279 (2016), 2015 The adaptive immune system relies on the diversity of receptors expressed on the surface of B and T-cells to protect the organism from a vast amount of pathogenic threats. The proliferation and degradation dynamics of different cell types (B cells, T cells, naive, memory) is governed by a variety of antigenic and environmental signals, yet the observed
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