Results 31 to 40 of about 1,642,375 (296)

Experimental discovery of sRNAs in Vibrio cholerae by direct cloning, 5S/tRNA depletion and parallel sequencing

open access: yesNucleic Acids Research, 2009
Direct cloning and parallel sequencing, an extremely powerful method for microRNA (miRNA) discovery, has not yet been applied to bacterial transcriptomes. Here we present sRNA-Seq, an unbiased method that allows for interrogation of the entire small, non-
Jane M. Liu   +5 more
semanticscholar   +1 more source

Microarray Assistant Clone Organizer and Array Simulator [PDF]

open access: yesBioTechniques, 2002
Microarrays are extensively used in molecular biology experiments. While several vendors offer microarrays on a variety of platforms, many researchers prefer to use custom microarrays with a selected list of clones for their experiments. Many research centers have established core facilities for the production of custom microarrays.
openaire   +4 more sources

Estimating genetic variability in non-model taxa: a general procedure for discriminating sequence errors from actual variation. [PDF]

open access: yesPLoS ONE, 2010
Genetic variation is the driving force of evolution and as such is of central interest for biologists. However, inadequate discrimination of errors from true genetic variation could lead to incorrect estimates of gene copy number, population genetic ...
Karen Dawson   +2 more
doaj   +1 more source

Quantifying the accessibility of the metagenome by random expression cloning techniques.

open access: yesEnvironmental Microbiology, 2004
The exploitation of the metagenome for novel biocatalysts by functional screening is determined by the ability to express the respective genes in a surrogate host.
E. Gabor, W. Alkema, D. Janssen
semanticscholar   +1 more source

Shotgun Phage Display - Selection for Bacterial Receptins or other Exported Proteins

open access: yesBiological Procedures Online, 2003
Shotgun phage display cloning involves construction of libraries from randomly fragmented bacterial chromosomal DNA, cloned genes, or eukaryotic cDNAs, into a phagemid vector.
Jacobsson Karin   +3 more
doaj   +1 more source

Technosphere-Urban Society and its Problems [PDF]

open access: yesSHS Web of Conferences, 2021
The technosphere which is the artificial shell of the Earth can be considered the contemporary result of developing the society and the world as a whole.
Mamichev Mikhail, Dergacheva Elena
doaj   +1 more source

Seabream ghrelin: cDNA cloning, genomic organization and promoter studies [PDF]

open access: yesJournal of Endocrinology, 2006
Recent studies have indicated that ghrelin stimulates growth hormone release from the pituitary via the growth hormone secretagogue receptor (GHSR). We have previously isolated two GHSR subtypes from the pituitary of the black seabream Acanthopagrus schlegeli.
Yeung, Chung M.   +3 more
openaire   +5 more sources

Repetitive sequences and their organization on genomic clones of Zea mays [PDF]

open access: yesThe EMBO Journal, 1984
Fourteen recombinant clones from Zea mays were studied with regard to their composition of unique and repetitive sequences. Southern hybridization experiments were used to classify restriction fragments of the clones into a unique, middle or highly repetitive class of reiteration frequency.
Nancy S. Shepherd   +3 more
openaire   +3 more sources

Emerging technologies to create inducible and genetically defined porcine cancer models

open access: yesFrontiers in Genetics, 2016
There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic and physiological similarities with humans.
Lawrence B Schook   +6 more
doaj   +1 more source

Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes

open access: yesBiotechnology for Biofuels and Bioproducts, 2022
Background The robust model cyanobacterium Synechocystis PCC 6803 is increasingly explored for its potential to use solar energy, water and atmospheric CO2 for the carbon-neutral production of terpenes, the high-value chemicals that can be used for the ...
Victoire Blanc-Garin   +6 more
doaj   +1 more source

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