Results 61 to 70 of about 1,786,363 (358)
Simple, Directional cDNA Cloning for In Situ Transcript Hybridization Screens
BioTechniques, 2001 Here, we describe a suppression PCR-based method for directional cloning of randomly primed cDNAs from small quantities of tissue. Synthesis of the first cDNA strand is conducted on oligonucleotide-coated magnetic beads. Synthesis of the second strand is
R. Tamme +4 more
doaj +1 more source
AMB Express, 2020 Meyerozyma guilliermondii, a non-conventional yeast that naturally assimilates xylose, is considered as a candidate for biotechnological production of the sugar alternative xylitol. Because the genes of the xylose metabolism were yet unknown, all efforts
Denise Atzmüller +2 more
doaj +1 more source
Journal of Biological Chemistry, 1998 Upon feeding, mosquito midguts secrete the peritrophic matrix (PM), an extracellular chitin-containing envelope that completely surrounds the blood meal. Because the malaria parasite must cross the PM to complete its life cycle in the mosquito, the PM is
Zhicheng Shen, M. Jacobs-Lorena
semanticscholar +1 more source
Physiology, structure, and regulation of the cloned organic anion transporters [PDF]
Xenobiotica, 2008 1. The transport of negatively charged drugs, xenobiotics, and metabolites by epithelial tissues, particularly the kidney, plays critical roles in controlling their distribution, concentration, and retention in the body. Thus, organic anion transporters (OATs) impact both their therapeutic efficacy and potential toxicity. 2.
C, Srimaroeng +2 more
openaire +2 more sources
Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions
FEBS Letters, EarlyView.We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva +10 more
wiley +1 more source
A genomic approach to the study of Tribolium castaneum genetics, development & evolution [PDF]
, 2004 During the last decade, Tribolium castaneum has become the insect of choice for comparative genetics and developmental studies outside of drosophilids. Until recently, most molecular studies have focused on the comparative analysis of early development ...
Savard, Joël
core
Cell wall target fragment discovery using a low‐cost, minimal fragment library
FEBS Letters, EarlyView.LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan +5 more
wiley +1 more source
Cytochrome c 6-like protein as a putative donor of electrons to photosystem I in the cyanobacterium Nostoc sp. PCC 7119 [PDF]
, 2011 Most organisms performing oxygenic photosynthesis contain either cytochrome c 6 or plastocyanin, or both, to transfer electrons from cytochrome b 6-f to photosystem I. Even though plastocyanin has superseded cytochrome c 6 along evolution, plants contain
Gil Martínez, Jorge +2 more
core +1 more source
Humanidades Médicas, 2003 La biotecnología, aunque tan antigua como la invención del vino y la fermentación de la cerveza por el hombre, tiene en los años 60 del siglo XX, un importante despegue con las técnicas de biología molecular y clonaje de genes foráneos en organismos ...
Ayní Rodríguez Pargas +3 more
doaj
Mos1 Element-Mediated CRISPR Integration of Transgenes in Caenorhabditis elegans
G3: Genes, Genomes, Genetics, 2019 The introduction of exogenous genes in single-copy at precise genomic locations is a powerful tool that has been widely used in the model organism Caenorhabditis elegans.
Nicholas S. Philip +4 more
doaj +1 more source