Results 91 to 100 of about 1,327 (168)

A TetR-Family Protein (CAETHG_0459) Activates Transcription From a New Promoter Motif Associated With Essential Genes for Autotrophic Growth in Acetogens

Frontiers in Microbiology, 2019
Acetogens can fix carbon (CO or CO2) into acetyl-CoA via the Wood–Ljungdahl pathway (WLP) that also makes them attractive cell factories for the production of fuels and chemicals from waste feedstocks.
Renato de Souza Pinto Lemgruber, Kaspar Valgepea, Kaspar Valgepea, Ricardo Axayacatl Gonzalez Garcia, Christopher de Bakker, Robin William Palfreyman, Robin William Palfreyman, Ryan Tappel, Michael Köpke, Séan Dennis Simpson, Lars Keld Nielsen, Esteban Marcellin, Esteban Marcellin   +12 more
doaj   +1 more source

Kinetic ensemble model of gas fermenting Clostridium autoethanogenum for improved ethanol production

Biochemical Engineering Journal, 2019
Abstract Developing autotrophic, acetogenic bacteria strains as gas fermentation platforms is a promising avenue for converting industrial waste gas streams into valuable chemical products. One such strain, Clostridium autoethanogenum, naturally converts CO, CO2, and H2 gases into ethanol and acetate.
Jennifer Greene, James Daniell, Michael Köpke, Linda Broadbelt, Keith E.J. Tyo   +4 more
openaire   +2 more sources

A novel experimental method to determine substrate uptake kinetics of gaseous substrates applied to the carbon monoxide‐fermenting Clostridium autoethanogenum

Biotechnology and Bioengineering, Volume 121, Issue 4, Page 1324-1334, April 2024.
This article describes a combination of static and dynamic cultivation of Clostridium autoethanogenum to study the substrate uptake kinetics of the gaseous substrate carbon monoxide. A steady‐state chemostat culture was periodically disturbed with 1‐h pulses of increased carbon monoxide partial pressures, up to 1.2 atm.
Maximilienne T. Allaart, Charilaos Korkontzelos, Diana Z. Sousa, Robbert Kleerebezem   +3 more
wiley   +1 more source

Implementation of a Clostridium luticellarii genome-scale model for upgrading syngas fermentations

Computational and Structural Biotechnology Journal
Syngas fermentation is a powerful platform for converting waste streams into sustainable carboxylic acid precursors for value-added biochemicals. Steel mills produce significant syngas, yet industrial microbial syngas valorization remains unrealized. The
William T. Scott, Jr., Siemen Rockx, Quinten Mariën, Alberte Regueira, Pieter Candry, Ramon Ganigué, Jasper J. Koehorst, Peter J. Schaap   +7 more
doaj   +1 more source

Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum

, 2022
The majority of the genes present in bacterial genomes remain poorly characterized, with up to one-third of those that are protein encoding having no definitive function. Transposon insertion sequencing represents a high-throughput technique that can help rectify this deficiency.
Woods, Craig, Humphreys, Christopher M., Tomi-Andrino, Claudio, Henstra, Anne M., Simpson, Sean D., Winzer, Klaus, Minton, Nigel P.   +6 more
openaire  

Metabolic engineering interventions for sustainable 2,3-butanediol production in gas fermenting Clostridium autoethanogenum

, 2023
Gas fermentation provides a promising platform to turn low-cost and readily available single-carbon waste gases into commodity chemicals such as 2,3-butanediol. Clostridium autoethanogenum is usually used as a robust and flexible chassis for gas fermentation.
Ghadermazi, Parsa, author, Chan, Siu Hung, advisor, Wrighton, Kelly, committee member, Reisfeld, Brad, committee member   +3 more
openaire   +1 more source

Advanced purification of isopropanol and acetone from syngas fermentation

Journal of Chemical Technology &Biotechnology, Volume 99, Issue 3, Page 714-726, March 2024.
Abstract BACKGROUND Isopropanol and acetone production by syngas fermentation is a promising alternative to conventional fossil carbon‐dependent production. However, this alternative technology has not yet been scaled up to an industrial level owing to the relatively low product concentrations (about 5 wt% in total).
Tamara Janković, Adrie JJ Straathof, Anton A Kiss   +2 more
wiley   +1 more source

Phylogenomics and genetic analysis of solvent-producing Clostridium species

Scientific Data
The genus Clostridium is a large and diverse group within the Bacillota (formerly Firmicutes), whose members can encode useful complex traits such as solvent production, gas-fermentation, and lignocellulose breakdown.
Rasmus O. Jensen, Frederik Schulz, Simon Roux, Dawn M. Klingeman, Wayne P. Mitchell, Daniel Udwary, Sarah Moraïs, Vinicio Reynoso, James Winkler, Shilpa Nagaraju, Sashini De Tissera, Nicole Shapiro, Natalia Ivanova, T. B. K. Reddy, Itzhak Mizrahi, Sagar M. Utturkar, Edward A. Bayer, Tanja Woyke, Nigel J. Mouncey, Michael C. Jewett, Séan D. Simpson, Michael Köpke, David T. Jones, Steven D. Brown   +23 more
doaj   +1 more source

MOESM1 of Genome editing of Clostridium autoethanogenum using CRISPR/Cas9

, 2016
Additional file 1. Sanger sequences; this file contains Sanger sequence of adh and 2,3-bdh loci from ∆adh clone T1.1, ∆adh clone T1.2 and ∆2,3-bdh clone T2.1.
Nagaraju, Shilpa, Davies, Naomi, Walker, David, Köpke, Michael, Séan Simpson   +4 more
openaire   +1 more source

Steerable isobutyric and butyric acid production from CO2 and H2 by Clostridium luticellarii

Microbial Biotechnology, Volume 17, Issue 1, January 2024.
Clostridium luticellarii is a highly flexible microorganism, able to reverse its (reverse) beta‐oxidation pathway depending on the pH. At circumneutral pH, C. luticellarii produces acetic acid from H2 and CO2 but is able to co‐oxidize organic acids and isomerize butyric acid. At mildly acidic pH (~5.5), C.
Quinten Mariën, Alberte Regueira, Ramon Ganigué   +2 more
wiley   +1 more source