Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose [PDF]
Biotechnology for Biofuels, 2019 Background Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. In particular, Clostridium thermocellum is a promising host for consolidated bioprocessing (CBP)
Liang Tian +6 more
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mSystems, 2020 Clostridium thermocellum and Thermoanaerobacterium saccharolyticum are thermophilic anaerobic bacteria with complementary metabolic capabilities that utilize distinct glycolytic pathways for the conversion of cellulosic sugars to biofuels.
Tyler B. Jacobson +7 more
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Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers [PDF]
Biotechnology for Biofuels and BioproductsBackground Clostridium thermocellum is a promising candidate for production of cellulosic biofuels, however, its final product titer is too low for commercial application, and this may be due to thermodynamic limitations in glycolysis.
Bishal Dev Sharma +7 more
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Identifying promoters for gene expression in Clostridium thermocellum
Metabolic Engineering Communications, 2015 A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. Here we test 17
Daniel G. Olson +5 more
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Laboratory Evolution and Reverse Engineering of Clostridium thermocellum for Growth on Glucose and Fructose. [PDF]
Appl Environ Microbiol, 2021 Yayo J +5 more
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Electrotransformation of Clostridium thermocellum [PDF]
Applied and Environmental Microbiology, 2004 ABSTRACT Electrotransformation of several strains of Clostridium thermocellum was achieved using plasmid pIKm1 with selection based on resistance to erythromycin and lincomycin. A custom-built pulse generator was used to apply a square 10-ms pulse to an electrotransformation cuvette consisting of a ...
Michael V, Tyurin +2 more
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Atypical Glycolysis in Clostridium thermocellum [PDF]
Applied and Environmental Microbiology, 2013 ABSTRACTCofactor specificities of glycolytic enzymes inClostridium thermocellumwere studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate (PPi)-linked activity ...
Zhou, J. (author) +6 more
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Adherence of Clostridium thermocellum to cellulose [PDF]
Journal of Bacteriology, 1983 The adherence of Clostridium thermocellum, a cellulolytic, thermophilic anaerobe, to its insoluble substrate (cellulose) was studied. The adherence phenomenon was determined to be selective for cellulose. The observed adherence was not significantly affected by various parameters, including salts, pH, temperature, detergents, or soluble sugars.
E A, Bayer, R, Kenig, R, Lamed
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Isolation of Clostridium thermocellum Auxotrophs [PDF]
Applied and Environmental Microbiology, 1982 Spontaneous and UV irradiation-induced auxotrophic mutants of Clostridium thermocellum , an anaerobic cellulolytic thermophile, were isolated after penicillin enrichment in a chemically defined medium.
B S, Méndez, R F, Gómez
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Thermostable chaperonin from Clostridium thermocellum [PDF]
Biochemical Journal, 1996 Homologues of the chaperonins Cpn60 and Cpn10 have been purified from the Gram-positive cellulolytic thermophile Clostridium thermocellum. The Cpn60 protein was purified by ATP-affinity chromatography and the Cpn10 protein was purified by gel-filtration, ion-exchange and hydrophobic interaction chromatographies.
S J, Cross +4 more
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