Results 31 to 40 of about 4,623 (199)

Characterization of Clostridium thermocellum JW20 [PDF]

Applied and Environmental Microbiology, 1988
Clostridium thermocellum JW20 (ATCC 31549), which was isolated from a Louisiana cotton bale, grew on cellulose, cellobiose, and xylooligomers and, after adaptation, on glucose, fructose, and xylose in the pH range of 7.5 to 6.1 with T opt of 60 C, T max of ...
Doris, Freier, Cheryle P, Mothershed, Juergen, Wiegel   +2 more
openaire   +2 more sources

Nanoscale resolution of microbial fiber degradation in action

eLife, 2022
The lives of microbes unfold at the micron scale, and their molecular machineries operate at the nanoscale. Their study at these resolutions is key toward achieving a better understanding of their ecology.
Meltem Tatli, Sarah Moraïs, Omar E Tovar-Herrera, Yannick J Bomble, Edward A Bayer, Ohad Medalia, Itzhak Mizrahi   +6 more
doaj   +1 more source

Development of a plasmid-based expression system in Clostridium thermocellum and its use to screen heterologous expression of bifunctional alcohol dehydrogenases (adhEs)

Metabolic Engineering Communications, 2016
Clostridium thermocellum is a promising candidate for ethanol production from cellulosic biomass, but requires metabolic engineering to improve ethanol yield.
Shuen Hon, Anthony A. Lanahan, Liang Tian, Richard J. Giannone, Robert L. Hettich, Daniel G. Olson, Lee R. Lynd   +6 more
doaj   +1 more source

Form and Function of Clostridium thermocellum Biofilms [PDF]

Applied and Environmental Microbiology, 2013
ABSTRACT The importance of bacterial adherence has been acknowledged in microbial lignocellulose conversion studies; however, few reports have described the function and structure of biofilms supported by cellulosic substrates. We investigated the organization, dynamic formation, and carbon flow associated with biofilms of the ...
Alexandru, Dumitrache, Gideon, Wolfaardt, Grant, Allen, Steven N, Liss, Lee R, Lynd   +4 more
openaire   +2 more sources

Glycolysis without pyruvate kinase in Clostridium thermocellum [PDF]

Metabolic Engineering, 2017
The metabolism of Clostridium thermocellum is notable in that it assimilates sugar via the EMP pathway but does not possess a pyruvate kinase enzyme. In the wild type organism, there are three proposed pathways for conversion of phosphoenolpyruvate (PEP) to pyruvate, which differ in their cofactor usage. One path uses pyruvate phosphate dikinase (PPDK),
Daniel G, Olson, Manuel, Hörl, Tobias, Fuhrer, Jingxuan, Cui, Jilai, Zhou, Marybeth I, Maloney, Daniel, Amador-Noguez, Liang, Tian, Uwe, Sauer, Lee R, Lynd   +9 more
openaire   +2 more sources

Elimination of formate production in Clostridium thermocellum [PDF]

Journal of Industrial Microbiology and Biotechnology, 2015
Abstract The ability of Clostridium thermocellum to rapidly degrade cellulose and ferment resulting hydrolysis products into ethanol makes it a promising platform organism for cellulosic biofuel production via consolidated bioprocessing.
Rydzak, Thomas, Lynd, Lee R., Guss, Adam M.   +2 more
openaire   +2 more sources

Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature

Metabolic Engineering Communications, 2016
Clostridium thermocellum is a leading candidate for the consolidated bioprocessing of lignocellulosic biomass for the production of fuels and chemicals.
Joseph Groom, Daehwan Chung, Daniel G. Olson, Lee R. Lynd, Adam M. Guss, Janet Westpheling   +5 more
doaj   +1 more source

Potential of thin stillage as a low-cost nutrient source for direct cellulose fermentation by Clostridium thermocellum

AIMS Energy, 2015
Utilization of thin stillage (TS), derived from grain-based ethanol production, was investigated as an alternative source for microbial growth nutrients during direct conversion of cellulose by Clostridium thermocellum DSM 1237. Fermentation end-products
Rumana Islam, Charushi Panditharatne, John Schellenberg, Richard Sparling, Nazim Cicek, David B. Levin   +5 more
doaj   +1 more source

Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms

BMC Microbiology, 2012
Background Clostridium thermocellum is an anaerobic thermophilic bacterium that exhibits high levels of cellulose solublization and produces ethanol as an end product of its metabolism.
Mearls Elizabeth B, Izquierdo Javier A, Lynd Lee R   +2 more
doaj   +1 more source

Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production

Biotechnology for Biofuels, 2018
Background Clostridium thermocellum has been the subject of multiple metabolic engineering strategies to improve its ability to ferment cellulose to ethanol, with varying degrees of success. For ethanol production in C.
Shuen Hon, Evert K. Holwerda, Robert S. Worthen, Marybeth I. Maloney, Liang Tian, Jingxuan Cui, Paul P. Lin, Lee R. Lynd, Daniel G. Olson   +8 more
doaj   +1 more source