The structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Keishi Ishida +9 more
wiley +2 more sources
Tracking the cellulolytic activity of Clostridium thermocellum biofilms [PDF]
Background Microbial cellulose conversion by Clostridium thermocellum 27405 occurs predominantly through the activity of substrate-adherent bacteria organized in thin, primarily single cell-layered biofilms. The importance of cellulosic surface exposure to microbial hydrolysis has received little attention despite its implied impact on conversion ...
Dumitrache, Alexandru +4 more
openaire +4 more sources
LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313 [PDF]
ABSTRACT Organisms regulate gene expression in response to the environment to coordinate metabolic reactions. Clostridium thermocellum expresses enzymes for both lignocellulose solubilization and its fermentation to produce ethanol. One LacI regulator termed GlyR3 in C. thermocellum
Charlotte M, Wilson +6 more
openaire +2 more sources
Physiology of Ethanol Production by Clostridium thermocellum
Dinesh Arya +2 more
openalex +2 more sources
Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and Pelosinus fermentans R7 [PDF]
ABSTRACT We and others have shown the utility of long sequence reads to improve genome assembly quality. In this study, we generated PacBio DNA sequence data to improve the assemblies of draft genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and ...
Sagar M. Utturkar +24 more
openaire +4 more sources
Logic‐Gated HSV‐TK/GCV Suicide Gene Circuit for Triple‐Negative Breast Cancer
The BRAS comprises two modular genetic components driven by distinct tumor‐specific promoters and a failsafe layer with the NOT gate. This multi‐input logic gate circuit enables precise, specific expression of HSV‐TK in breast cancer cells with hardly expression in normal cell and effectively inhibits tumor growth in a triple‐negative breast cancer ...
Shasha Tang +10 more
wiley +1 more source
Background Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from natural variants are not clear.
Vanessa A. Thomas +11 more
doaj +1 more source
Differential metabolism of cellobiose and glucose by Clostridium thermocellum and Clostridium thermohydrosulfuricum [PDF]
Clostridium thermohydrosulfuricum consumed glucose in preference to cellobiose as an energy source for growth. The rates of substrate uptake in glucose- and cellobiose-grown cell suspensions were 45 and 24 nmol/min per mg (dry weight), respectively, at 65 degrees C.
T K, Ng, J G, Zeikus
openaire +2 more sources
Hydrogen fuel production from sugarcane and its byproducts – a critical review
Abstract The transition to a low‐carbon economy has intensified the search for sustainable hydrogen (H2) production routes. Sugarcane and its byproducts, such as bagasse, molasses and vinasse, have emerged as promising feedstocks due to their ready availability and renewable nature. However, comprehensive evaluations of the efficiency, limitations, and
Jaqueline Calixto de Sousa +3 more
wiley +1 more source
Cereal Arabinoxylans—Their Enzymatic Degradation and Relevance for Breadmaking and Human Health
ABSTRACT As the most abundant nonstarch polysaccharides in cereals, arabinoxylans (AXs) contribute significantly to the global intake of dietary fiber. They play a crucial role in the breadmaking process with respect to dough rheology and texture, bread volume, and nutritional quality, especially when starting from wheat or rye flour.
Víctor González‐Alonso +3 more
wiley +1 more source

