Results 51 to 60 of about 4,623 (199)

Partial Purification of Cellulase from Clostridium thermocellum [PDF]

Journal of General Microbiology, 1979
Summary: The cellulolytic system from culture filtrates of Clostridium thermocellum, when partially purified by preparative electrophoresis, showed a single protein band on analytical polyacrylamide gel electrophoresis; however, sodium dodecyl sulphate-polyacrylamide gel electrophoresis indicated that at least five proteins were present.
N. AIT, N. CREUZET, P. FORGET
openaire   +1 more source

Training bias and sequence alignments shape protein–peptide docking by AlphaFold and related methods

Protein Science, Volume 34, Issue 11, November 2025.
Abstract Protein‐peptide interactions mediate many biological processes. Accurate structural models of protein‐peptide complexes, determined by experiment or computational prediction, are essential for understanding function and designing interaction inhibitors.
Lindsey Guan, Amy E. Keating
wiley   +1 more source

Increase in ethanol yield via elimination of lactate production in an ethanol-tolerant mutant of Clostridium thermocellum.

PLoS ONE, 2014
Large-scale production of lignocellulosic biofuel is a potential solution to sustainably meet global energy needs. One-step consolidated bioprocessing (CBP) is a potentially advantageous approach for the production of biofuels, but requires an organism ...
Ranjita Biswas, Sandeep Prabhu, Lee R Lynd, Adam M Guss   +3 more
doaj   +1 more source

Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol

Biotechnology for Biofuels, 2017
Background Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; however, the low ethanol titer produced by strains developed thus far is an obstacle to industrial application ...
Liang Tian, Skyler J. Perot, David Stevenson, Tyler Jacobson, Anthony A. Lanahan, Daniel Amador-Noguez, Daniel G. Olson, Lee R. Lynd   +7 more
doaj   +1 more source

Biological Routes for Biohydrogen Production: A Clean and Carbon‐Free Fuel

Biotechnology Journal, Volume 20, Issue 7, July 2025.
Graphical Abstract and Lay Summary Biological routes for hydrogen (H2) production are mainly classified into four pathways: 1) direct bio‐photolysis, 2) indirect bio‐photolysis, 3) photo‐fermentation, and 4) dark fermentation. Of these pathways, dark fermentation produces the highest levels of H2 production.
Minseok Cha, Min‐Seo Park, Soo‐Jung Kim   +2 more
wiley   +1 more source

Decoding Biomass-Sensing Regulons of Clostridium thermocellum Alternative Sigma-I Factors in a Heterologous Bacillus subtilis Host System.

PLoS ONE, 2016
The Gram-positive, anaerobic, cellulolytic, thermophile Clostridium (Ruminiclostridium) thermocellum secretes a multi-enzyme system called the cellulosome to solubilize plant cell wall polysaccharides.
Iván Muñoz-Gutiérrez, Lizett Ortiz de Ora, Inna Rozman Grinberg, Yuval Garty, Edward A Bayer, Yuval Shoham, Raphael Lamed, Ilya Borovok   +7 more
doaj   +1 more source

Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum

Biotechnology for Biofuels, 2018
Background Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C.
Ya-Jun Liu, Shiyue Liu, Sheng Dong, Renmin Li, Yingang Feng, Qiu Cui   +5 more
doaj   +1 more source

Clostridium thermocellum ATCC27405 transcriptomic, metabolomic and proteomic profiles after ethanol stress

BMC Genomics, 2012
Background Clostridium thermocellum is a candidate consolidated bioprocessing biocatalyst, which is a microorganism that expresses enzymes for both cellulose hydrolysis and its fermentation to produce fuels such as lignocellulosic ethanol.
Yang Shihui, Giannone Richard J, Dice Lezlee, Yang Zamin K, Engle Nancy L, Tschaplinski Timothy J, Hettich Robert L, Brown Steven D   +7 more
doaj   +1 more source

Grape Stalks Valorization towards Circular Economy: A Cascade Biorefinery Strategy

ChemSusChem, Volume 18, Issue 10, May 19, 2025.
A cascade protocol for grape stalks valorization has been proposed, characterized by a multidisciplinary strategy using unconventional techniques and subcritical water as a green solvent, where physical/chemical treatments synergistically interact with biological treatments to obtain added value products from all the biomass components.
Carlotta Valle, Giorgio Grillo, Emanuela Calcio Gaudino, Paola Ponsetto, Roberto Mazzoli, Giulia Bonavita, Pietro Vitale, Enrica Pessione, Emilia Garcia‐Moruno, Antonella Costantini, Giancarlo Cravotto, Silvia Tabasso   +11 more
wiley   +1 more source

Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum

Biotechnology for Biofuels, 2017
Background Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and ...
Jie Zhang, Shiyue Liu, Renmin Li, Wei Hong, Yan Xiao, Yingang Feng, Qiu Cui, Ya-Jun Liu   +7 more
doaj   +1 more source