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Major protein components in the cell envelope of Clostridium Tyrobutyricum

Annales de l'Institut Pasteur / Microbiologie, 1986
The overall composition of the Clostridium tyrobutyricum cell envelope did not vary significantly during cell growth and was characterized by a high protein content (about 40% dry weight). Teichoic and teichuronic acids were absent and the neutral sugar content low. Insoluble peptidoglycan represented only 10-12% of the cell envelope (dry weight basis);
Bergere, J.L.   +2 more
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Effects of copper on germination, growth and sporulation of Clostridium tyrobutyricum

Food Microbiology, 2010
The effects of copper (Cu(2+)) on spore germination, vegetative growth and sporulation of Clostridium tyrobutyricum, which is capable to causing texture and flavour defects in Emmental cheese, were studied. Spore suspensions of three different strains were used as starting material for two experimental set-ups.
L, Mato Rodriguez, T, Alatossava
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Metabolic engineering of Clostridium tyrobutyricum for n-butanol production

Metabolic Engineering, 2011
Clostridium tyrobutyricum ATCC 25755, a butyric acid producing bacterium, has been engineered to overexpress aldehyde/alcohol dehydrogenase 2 (adhE2, Genebank no. AF321779) from Clostridium acetobutylicum ATCC 824, which converts butyryl-CoA to butanol, under the control of native thiolase (thl) promoter.
Mingrui, Yu   +3 more
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Inhibition of Clostridium tyrobutyricum in cheese by Lactobacillus gasseri

International Dairy Journal, 2007
Abstract A semi-hard cheese produced from milk artificially contaminated with Clostridium tyrobutyricum spores (2.5×103 mL−1) was used as a model for studying the ability of bacteriocin-producing Lactobacillus gasseri K7 (Rifr) to inhibit clostridia. The added lactobacilli did not inhibit the primary starter culture (Streptococcus thermophilus), but ...
B. Bogovič Matijašić   +3 more
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Thermorésistance des spores declostridium tyrobutyricumetClostridium butyricum

Journal of Dairy Research, 1967
SummaryClean spores suspensions of 3 strains ofClostridium tyrobutyricumwere prepared after culture in dialysis sacs. The survivor curves of the spores heated at 90 °C in 0·033 M phosphate buffer (pH 7·0) or in skim-milk were logarithmic. From the thermal-death-time curves the following values were calculated:Dat 121 °C = 0·003−0·012 min andz= 8·4−10 ...
Cerf, O., Bergere, J.L., Hermier, J.
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High production of butyric acid by Clostridium tyrobutyricum mutant

Frontiers of Chemical Science and Engineering, 2015
The objective of this study was to improve the production of butyric acid by process optimization using the metabolically engineered mutant of Clostridium tyrobutyricum (PAK-Em). First, the free-cell fermentation at pH 6.0 produced butyric acid with concentration of 38.44 g/L and yield of 0.42 g/g.
Chao Ma   +4 more
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Inhibition ofClostridium Tyrobutyricumin cheese-slurry

Acta Alimentaria, 2010
Effects of muramidase, potassium nitrate, the mesophilic starter culture, nisin, the nisin-producing Lactococcus strain and two Lactobacillus strains with anticlostridial activity on survival and growth of the gas-producing Clostridium tyrobutyricum in a model cheese-slurry system were compared.Control cheese-slurry sample and samples with ...
Němečková, I.   +7 more
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Comparative transcriptome analysis of Clostridium tyrobutyricum expressing a heterologous uptake hydrogenase

Science of The Total Environment, 2020
Clostridium tyrobutyricum is a promising microbial cell factory to produce biofuels. In this study, an uptake hydrogenase (hyd2293) from Ethanoligenens harbinense was overexpressed in C. tyrobutyricum and significantly affected the redox reactions and metabolic profiles. Compared to the parental strain (Ct-WT), the mutant strain Ct-Hyd2293 produced ~34%
Weiming Li   +4 more
openaire   +2 more sources

Immuno-based strategy for Clostridium tyrobutyricum specific recognition

Biotechnology Techniques, 1993
Polyclonal antibodies of C.tyrobutyricum were prepared and coupled to an immunoadsorbent resin specific for this microorganism contaminant of cheese milk. The immunoadsorbent selectivity was evaluated also by scanning electron microscope an aqueous suspension of C.tyrobutyricum vegetative cells.
SCOLARI G. L.   +5 more
openaire   +1 more source

Metabolic engineering of Clostridium tyrobutyricum for hexanoic acid and hexanol production

Bioresource Technology
n-Hexanoic acid and n-hexanol, a six-carbon linear carboxylic acid and its corresponding alcohol, are important building blocks for pharmaceuticals, cosmetics, fuels, and food additives. Microbial biosynthesis offers a sustainable alternative to petroleum-based production, yet yields are often constrained by product toxicity and limited pathway ...
Shangjun Wang   +5 more
openaire   +2 more sources

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