Results 51 to 60 of about 1,732,242 (271)

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Methods for Enhancing Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Homology-Directed Repair Efficiency

Frontiers in Genetics, 2019
The evolution of organisms has provided a variety of mechanisms to maintain the integrity of its genome, but as damage occurs, DNA damage repair pathways are necessary to resolve errors.
Xi-Dian Tang, Fei Gao, Ming-Jie Liu, Qin-Lei Fan, De-Kun Chen, Wen-Tao Ma   +5 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Stress-induced DNA Damage biomarkers: Applications and limitations

Frontiers in Chemistry, 2015
A variety of environmental stresses like chemicals, UV and ionizing radiation and organism’s endogenous processes like replication stress and metabolism can lead to the generation of reactive oxygen and nitrogen species (ROS/RNS) that can attack cellular
Zacharenia eNikitaki, christine E Hellweg, Alexandros G Georgakilas, Jean-Luc eRAVANAT, Jean-Luc eRAVANAT   +4 more
doaj   +1 more source

Role of Base Excision Repair Pathway in the Processing of Complex DNA Damage Generated by Oxidative Stress and Anticancer Drugs

Frontiers in Cell and Developmental Biology, 2021
Chemical alterations in DNA induced by genotoxic factors can have a complex nature such as bulky DNA adducts, interstrand DNA cross-links (ICLs), and clustered DNA lesions (including double-strand breaks, DSB).
Yeldar Baiken, Yeldar Baiken, Yeldar Baiken, Damira Kanayeva, Sabira Taipakova, Regina Groisman, Alexander A. Ishchenko, Dinara Begimbetova, Bakhyt Matkarimov, Murat Saparbaev, Murat Saparbaev   +10 more
doaj   +1 more source

Damage-Cluster Distributions and Size Effect on Strength in Compressive Failure [PDF]

Physical Review Letters, 2012
We investigate compressive failure of heterogeneous materials on the basis of a continuous progressive damage model. The model explicitely accounts for tensile and shear local damage and reproduces the main features of compressive failure of brittle materials like rocks or ice.
Girard, Lucas, Weiss, Jérôme, Amitrano, David   +2 more
openaire   +6 more sources

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Piezoelectric Active Sensor Self-Diagnosis for Electromechanical Impedance Monitoring Using K-Means Clustering Analysis and Artificial Neural Network

Shock and Vibration, 2021
Piezoelectric sensor is a crucial part of electromechanical impedance technology whose state will directly affect the effectiveness and accuracy of structural health monitoring (SHM).
Xie Jiang, Xin Zhang, Yuxiang Zhang
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Emerging experimental and computational methods for studying redox‐regulated structural transitions

FEBS Letters, EarlyView.
Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass, Dana Reichmann, Gábor Erdős   +2 more
wiley   +1 more source