Results 21 to 30 of about 4,207 (127)

The Genotoxin Colibactin Shapes Gut Microbiota in Mice

mSphere, 2020
The genotoxin colibactin produced by resident bacteria of the gut microbiota may have tumorigenic effect by inducing DNA double-strand breaks in host cells. Yet, the effect of colibactin on gut microbiota composition and functions remains unknown.
Sophie Tronnet, Pauline Floch, Laetitia Lucarelli, Deborah Gaillard, Patricia Martin, Matteo Serino, Eric Oswald   +6 more
doaj   +3 more sources

The Food Contaminant Deoxynivalenol Exacerbates the Genotoxicity of Gut Microbiota

mBio, 2017
An increasing number of human beings from developed countries are colonized by Escherichia coli strains producing colibactin, a genotoxin suspected to be associated with the development of colorectal cancers.
Delphine Payros, Ulrich Dobrindt, Patricia Martin, Thomas Secher, Ana Paula F. L. Bracarense, Michèle Boury, Joelle Laffitte, Philippe Pinton, Eric Oswald, Isabelle P. Oswald   +9 more
doaj   +1 more source

A Toxic Friend: Genotoxic and Mutagenic Activity of the Probiotic Strain Escherichia coli Nissle 1917

mSphere, 2021
Nissle 1917 is sold as a probiotic and considered safe even though it has been known since 2006 that it harbors the genes for colibactin synthesis. Colibactin is a potent genotoxin that is now linked to causative mutations found in human colorectal ...
Jean-Philippe Nougayrède, Camille V. Chagneau, Jean-Paul Motta, Nadège Bossuet-Greif, Marcy Belloy, Frédéric Taieb, Jean-Jacques Gratadoux, Muriel Thomas, Philippe Langella, Eric Oswald   +9 more
doaj   +1 more source

Uropathogenic E. coli induces DNA damage in the bladder.

PLoS Pathogens, 2021
Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product ...
Camille V Chagneau, Clémence Massip, Nadège Bossuet-Greif, Christophe Fremez, Jean-Paul Motta, Ayaka Shima, Céline Besson, Pauline Le Faouder, Nicolas Cénac, Marie-Paule Roth, Hélène Coppin, Maxime Fontanié, Patricia Martin, Jean-Philippe Nougayrède, Eric Oswald   +14 more
doaj   +1 more source

Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using γ-H2AX as a Marker

Bio-Protocol, 2018
The genotoxin colibactin is produced by several species of Enterobacteriaceae. This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells (Nougayrède et al., 2006; Taieb et al., 2016).
Sophie Tronnet, Eric Oswald
doaj   +1 more source

Protocol for HeLa Cells Infection with Escherichia coli Strains Producing Colibactin and Quantification of the Induced DNA-damage

Bio-Protocol, 2017
Strains of Escherichia coli bearing the pks genomic island synthesize the genotoxin colibactin. Exposure of eukaryotic cells to E. coli producing colibactin induces DNA damages, ultimately leading to cell cycle arrest, senescence and death.
Nadège Bossuet-Greif, Marcy Belloy, Michèle Boury, Eric Oswald, Jean-Philippe Nougayrede   +4 more
doaj   +1 more source

Genome-scale CRISPR/Cas9 screening reveals the role of PSMD4 in colibactin-mediated cell cycle arrest

mSphere
Colibactin is a genotoxic secondary metabolite produced by certain Enterobacteriaceae strains that populate the intestine and produces a specific mutational signature in human colonocytes. However, the host pathways involved in colibactin response remain
Michael W. Dougherty, Ryan M. Hoffmann, Maria C. Hernandez, Yougant Airan, Raad Z. Gharaibeh, Seth B. Herzon, Ye Yang, Christian Jobin   +7 more
doaj   +1 more source

Colibactin possessing E. coli isolates in association with colorectal cancer and their genetic diversity among Pakistani population.

PLoS ONE, 2022
Colorectal cancer (CRC) is the third most prevalent cause of tumorigenesis and several pathogenic bacteria have been correlated with aggressive cases of cancer i.e., genotoxin (colibactin) producing Escherichia coli (E. coli).
Habiba Tariq, Zobia Noreen, Aftab Ahmad, Laraib Khan, Mashhood Ali, Muhammad Malik, Aneela Javed, Faisal Rasheed, Alina Fatima, Tanil Kocagoz, Ugur Sezerman, Habib Bokhari   +11 more
doaj   +1 more source

L-tryptophan and copper interactions linked to reduced colibactin genotoxicity in pks+ Escherichia coli

mSystems
Colibactin, a nonribosomal peptide/polyketide produced by pks+ Enterobacteriaceae, is a virulence factor and putative carcinogen that damages DNA by interstrand crosslinking (ICL).
Charlie Bayne, Magali Boutard, Tom Zaplana, Andrew C. Tolonen   +3 more
doaj   +1 more source

Genetic and Functional Analysis of the pks Gene in Clinical Klebsiella pneumoniae Isolates

Microbiology Spectrum, 2023
The pks gene cluster encodes colibactin, which can cause DNA damage and enhance the virulence in Escherichia coli. However, the role of the pks gene in Klebsiella pneumoniae has not been fully discussed.
Chenshuo Luo, Yanshu Chen, Xueni Hu, Shanjian Chen, Yulan Lin, Xiaoqian Liu, Bin Yang   +6 more
doaj   +1 more source