Results 41 to 50 of about 5,208 (185)

Depurination of colibactin-derived interstrand cross-links [PDF]

Biochemistry, 2019
Abstract Colibactin is a genotoxic gut microbiome metabolite long suspected of playing an etiological role in colorectal cancer progression. Evidence suggests colibactin forms DNA interstrand cross-links (ICLs) in eukaryotic cells and activates ICL repair pathways, leading to the production of ICL-dependent DNA double-strand breaks ...
Mengzhao Xue, Kevin M. Wernke, Seth B. Herzon   +2 more
openaire   +2 more sources

Quantification of Colibactin-associated Genotoxicity in HeLa Cells by In Cell Western (ICW) Using γ-H2AX as a Marker

Bio-Protocol, 2018
The genotoxin colibactin is produced by several species of Enterobacteriaceae. This genotoxin induces DNA damage, cell cycle arrest, senescence and death in eukaryotic cells (Nougayrède et al., 2006; Taieb et al., 2016).
Sophie Tronnet, Eric Oswald
doaj   +1 more source

Uropathogenic E. coli induces DNA damage in the bladder.

PLoS Pathogens, 2021
Urinary tract infections (UTIs) are among the most common outpatient infections, with a lifetime incidence of around 60% in women. We analysed urine samples from 223 patients with community-acquired UTIs and report the presence of the cleavage product ...
Camille V Chagneau, Clémence Massip, Nadège Bossuet-Greif, Christophe Fremez, Jean-Paul Motta, Ayaka Shima, Céline Besson, Pauline Le Faouder, Nicolas Cénac, Marie-Paule Roth, Hélène Coppin, Maxime Fontanié, Patricia Martin, Jean-Philippe Nougayrède, Eric Oswald   +14 more
doaj   +1 more source

A Toxic Friend: Genotoxic and Mutagenic Activity of the Probiotic Strain Escherichia coli Nissle 1917

mSphere, 2021
Nissle 1917 is sold as a probiotic and considered safe even though it has been known since 2006 that it harbors the genes for colibactin synthesis. Colibactin is a potent genotoxin that is now linked to causative mutations found in human colorectal ...
Jean-Philippe Nougayrède, Camille V. Chagneau, Jean-Paul Motta, Nadège Bossuet-Greif, Marcy Belloy, Frédéric Taieb, Jean-Jacques Gratadoux, Muriel Thomas, Philippe Langella, Eric Oswald   +9 more
doaj   +1 more source

Depurination of Colibactin-Derived Interstrand Cross-Links

, 2020
Colibactin is a genotoxic gut microbiome metabolite long suspected of playing an etiological role in colorectal cancer. Evidence suggests that colibactin forms DNA interstrand cross-links (ICLs) in eukaryotic cells and activates ICL repair pathways ...
Mengzhao Xue (3359471), Kevin M. Wernke (8442288), Seth B. Herzon (1319394)   +2 more
core   +1 more source

Protocol for HeLa Cells Infection with Escherichia coli Strains Producing Colibactin and Quantification of the Induced DNA-damage

Bio-Protocol, 2017
Strains of Escherichia coli bearing the pks genomic island synthesize the genotoxin colibactin. Exposure of eukaryotic cells to E. coli producing colibactin induces DNA damages, ultimately leading to cell cycle arrest, senescence and death.
Nadège Bossuet-Greif, Marcy Belloy, Michèle Boury, Eric Oswald, Jean-Philippe Nougayrede   +4 more
doaj   +1 more source

Colibactin

, 2020
Bakalářská práce shrnuje dosavadní poznatky o struktuře, syntéze, metabolitech a pozitivních účincích toxinu kolibaktinu, který je produkovaný bakteriemi Klebsiella pneumoniae, Enterobacter aerogenes, Citrobacter koseri a zejména Escherichia coli ...
Tylšová, Tereza
core   +1 more source

Δpks EcN does not produce colibactin.

, 2023
A) The maturation of colibactin by ClbP results in two molecules of N-myristoyl-D-asparagine, which can subsequently be used as biomarker for colibactin production in EcN strains.
Aida Kalantari (6731369), Mylene Perreault (2004913), Lauren A. Renaud (14559642), Mary N. McDonald (14559648), David L. Hava (14559651), Michael J. James (336941), Vincent M. Isabella (14559654), Catherine E. Monahan (14559645)   +7 more
core   +1 more source

ClbS Is a Cyclopropane Hydrolase That Confers Colibactin Resistance [PDF]

Journal of the American Chemical Society, 2017
Certain commensal Escherichia coli contain the clb biosynthetic gene cluster that codes for small molecule prodrugs known as precolibactins. Precolibactins are converted to colibactins by N-deacylation; the latter are postulated to be genotoxic and to contribute to colorectal cancer formation.
Prabhanshu Tripathi, Emilee E. Shine, Alan R. Healy, Chung Sub Kim, Seth B. Herzon, Steven D. Bruner, Jason M. Crawford   +6 more
openaire   +2 more sources

MATE transport of the E. coli-derived genotoxin colibactin [PDF]

Nature Microbiology, 2016
Various forms of cancer have been linked to the carcinogenic activities of microorganisms(1-3). The virulent gene island polyketide synthase (pks) produces the secondary metabolite colibactin, a genotoxic molecule(s) causing double-stranded DNA breaks(4) and enhanced colorectal cancer development(5,6).
Mousa, Jarrod J., Yang, Ye, Tomkovich, Sarah, Shima, Ayaka, Newsome, Rachel C., Tripathi, Prabhanshu, Oswald, Eric, Bruner, Steven D., Jobin, Christian   +8 more
openaire   +3 more sources