Results 31 to 40 of about 1,956,113 (201)
The Comet Assay: Tails of the (Un)expected. Use of the comet assay in pharmaceutical development.
Frontiers in Genetics, 2015 In genotoxicity testing of pharmaceuticals the rodent alkaline comet assay is being increasingly used as a second in vivo assay in addition to the in vivo micronucleus assay to mitigate in vitro positive results as recommended by regulatory guidance.
Bas-jan Van Der Leede
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Modification of comet-FISH technique by using temperature instead of chemical denaturation
MethodsX, 2014 Comet-FISH technique is an extension of commonly used comet assay. Its purpose is to determine whether primary DNA damage which comet assay detects occurred within a sequence of interest that is visualized by hybridization of fluorescent probe.
Marin Mladinic, Davor Zeljezic
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Closing the gap: from public concerns about health effects to science-based testing of chemicals
Frontiers in Genetics, 2015 The comet assay is a powerful technology for measuring DNA integrity in humans and other organisms at the level of individual cells. The assay is used for research and industrial testing but suffers from a lack of standardisation, which hinders its use ...
Sergey Shaposhnikov +2 more
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The basic principles of DNA damage detection by the alkaline comet assay [PDF]
Arhiv za farmacijuThe Comet assay is a highly sensitive and rapid method for detecting DNA damage at the level of individual cells. It measures single-stranded and double-stranded DNA breaks, alkali-labile, incomplete excision repair and cross-linking sites.
Živković Lada +6 more
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HiComet: a high-throughput comet analysis tool for large-scale DNA damage assessment
BMC Bioinformatics, 2018 Background DNA damage causes aging, cancer, and other serious diseases. The comet assay can detect multiple types of DNA lesions with high sensitivity, and it has been widely applied.
Taehoon Lee +7 more
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The use of comet assay to assess global DNA methylation in human biomonitoring studies.
Frontiers in Genetics, 2015 The Comet assay is a valuable tool for the detection of DNA damage in genotoxicity and human biomonitoring studies. Throughout the years, this biomarker has undergone several adaptations in their protocol in order to increase its sensitivity and the ...
Carla Costa +2 more
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The Iraqi Journal of Agricultural science, 2023 The colonies of C. glabrata were described, which morphology of isolated on sabouraud dextrose agar with chloramphenicol. Biosynthesis of nanoparticles is of great importance since they are highly involved in various medical and biological applications.
Teeba H. Mohammad
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Toxoplasma gondii infection can induce retinal DNA damage:an experimental study
International Journal of Ophthalmology, 2014 AIM: To detect whether Toxoplasma gondii (T. gondii) infection of mice can induce retinal DNA damage.METHODS: A total of 20 laboratory-bred male Swiss albino mice were used and divided into four groups:control group (non-infected animals); T ...
Nagwa Mostafa El-Sayed, Eman Mohamed Aly
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Genes and Environment, 2021 Background The in vivo comet assay is used to evaluate the genotoxic potential of compounds by detecting DNA strand breaks in cells isolated from animal tissue.
Haruna Tahara +3 more
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Comet assay and its use for evaluating oxidative DNA damage in some pathological states.
Physiological Research, 2019 The comet assay, or single-cell gel electrophoresis (SCGE), is a sensitive, rapid, relatively simple and inexpensive method for detecting DNA strand breaks in individual cells.
M. Kucharová +6 more
semanticscholar +1 more source