Results 41 to 50 of about 1,981,264 (328)
The comet assay: topical issues [PDF]
Mutagenesis, 2008 The comet assay is a versatile and sensitive method for measuring single- and double-strand breaks in DNA. The mechanism of formation of comets (under neutral or alkaline conditions) is best understood by analogy with nucleoids, in which relaxation of DNA supercoiling in a structural loop of DNA by a single DNA break releases that loop to extend into a
A. R. Collins +7 more
openaire +4 more sources
The use of comet assay to assess global DNA methylation in human biomonitoring studies.
Frontiers in Genetics, 2015 The Comet assay is a valuable tool for the detection of DNA damage in genotoxicity and human biomonitoring studies. Throughout the years, this biomarker has undergone several adaptations in their protocol in order to increase its sensitivity and the ...
Carla Costa +2 more
doaj +1 more source
The Iraqi Journal of Agricultural science, 2023 The colonies of C. glabrata were described, which morphology of isolated on sabouraud dextrose agar with chloramphenicol. Biosynthesis of nanoparticles is of great importance since they are highly involved in various medical and biological applications.
Teeba H. Mohammad
doaj +1 more source
Toxoplasma gondii infection can induce retinal DNA damage:an experimental study
International Journal of Ophthalmology, 2014 AIM: To detect whether Toxoplasma gondii (T. gondii) infection of mice can induce retinal DNA damage.METHODS: A total of 20 laboratory-bred male Swiss albino mice were used and divided into four groups:control group (non-infected animals); T ...
Nagwa Mostafa El-Sayed, Eman Mohamed Aly
doaj +1 more source
Genes and Environment, 2021 Background The in vivo comet assay is used to evaluate the genotoxic potential of compounds by detecting DNA strand breaks in cells isolated from animal tissue.
Haruna Tahara +3 more
doaj +1 more source
Comet assay and its use for evaluating oxidative DNA damage in some pathological states.
Physiological Research, 2019 The comet assay, or single-cell gel electrophoresis (SCGE), is a sensitive, rapid, relatively simple and inexpensive method for detecting DNA strand breaks in individual cells.
M. Kuchařová +6 more
semanticscholar +1 more source
Relationship between CpG and non-CpG DNA methylation in human lymphocytes assessed with comet assay [PDF]
The Ukrainian Biochemical JournalTissue-specific DNA methylation plays an important role in the regulation of many functional processes. The methylation level in single cells can be assessed using the comet assay (single-cell gel electrophoresis), a simple and cost-effective technique ...
M. Chopei +3 more
doaj +1 more source
The comet assay – from toy to tool
Frontiers in Genetics, 2015 The comet assay is nowadays the most common method for measuring DNA damage and repair in single cells. It is based on the microelectrophoretic study published by Ostling and Johanson (1984) and was developed by Singh and coworkers (1988) to a versatile ...
Guenter Speit
doaj +1 more source
Protective effects of phenolic acids on mercury-induced DNA damage in precision-cut kidney slices [PDF]
Iranian Journal of Basic Medical Sciences, 2019 Objective(s): Precision-cut tissue slices are considered an organotypic 3D model widely used in biomedical research. The comet assay is an important screening test for early genotoxicity risk assessment that is mainly applied on in vitro models. The aim
Irma Edith Carranza-Torres +7 more
doaj +1 more source
Laser‐Induced Graphene from Waste Almond Shells
Advanced Functional Materials, EarlyView.Almond shells, an abundant agricultural by‐product, are repurposed to create a fully bioderived almond shell/chitosan composite (ASC) degradable in soil. ASC is converted into laser‐induced graphene (LIG) by laser scribing and proposed as a substrate for transient electronics.
Yulia Steksova +9 more
wiley +1 more source