Results 1 to 10 of about 25,858 (250)

Improving Loop Modeling of the Antibody Complementarity-Determining Region 3 Using Knowledge-Based Restraints. [PDF]

PLoS ONE, 2016
Structural restrictions are present even in the most sequence diverse portions of antibodies, the complementary determining region (CDR) loops. Previous studies identified robust rules that define canonical structures for five of the six CDR loops ...
Jessica A Finn, Julia Koehler Leman, Jordan R Willis, Alberto Cisneros, James E Crowe, Jens Meiler   +5 more
doaj   +5 more sources

High-affinity optimization potential of the virus neutralizing antibody with twin cysteine-stabilized complementarity-determining region 3 [PDF]

Frontiers in Cellular and Infection Microbiology
IntroductionThe optimization of neutralizing monoclonal antibodies (NMAbs) is crucial to counter viral evolution. The structural stability of the heavy-chain complementarity-determining region 3 (H3 CDR) significantly influences affinity maturation ...
Jing Li, Dan-Dan Zeng, Qi Yin, Sen Zhang, Dong-Sheng Zhao, Yue Zhang, Zhang Zhang, Fan Tong, Zhong-Peng Zhao, Tao Jiang, Guang-Yu Zhao, Gang Dong   +11 more
doaj   +5 more sources

Investigating the IgM and IgG B Cell Receptor Repertoires and Expression of Ultralong Complementarity Determining Region 3 in Colostrum and Blood from Holstein-Friesian Cows at Calving [PDF]

Animals
In cattle, colostral maternal immunoglobulins and lymphocytes transfer across the neonate’s intestinal epithelium to provide protection against pathogens.
Tess E. Altvater-Hughes, Harold P. Hodgins, Douglas C. Hodgins, Cathy A. Bauman, Marlene A. Paibomesai, Bonnie A. Mallard   +5 more
doaj   +4 more sources

Molecular features of the complementarity determining region 3 motif of the T cell population and subsets in the blood of patients with chronic severe hepatitis B [PDF]

Journal of Translational Medicine, 2011
Background T cell receptor (TCR) reflects the status and function of T cells. We previously developed a gene melting spectral pattern (GMSP) assay, which rapidly detects clonal expansion of the T cell receptor β variable gene (TCRBV) in patients with HBV
Yang Jiezuan, He Jianqin, Lu Haifeng, Wei Li, Li Sujun, Wang Baohong, Diao Hongyan, Li Lanjuan   +7 more
doaj   +5 more sources

Expression of ultralong complementarity determining region 3 and development of IgM and IgG B cell receptor repertoires in Holstein heifer calves. [PDF]

Immunohorizons, 2022
Abstract There are currently knowledge gaps regarding the development of the bovine B cell repertoire especially for cells expressing B cell receptors with heavy chain ultralong complementarity determining region 3 (CDR3). The objective of this study was to assess percentages of B cells with ultralong CDR3s, and immunoglobulin heavy ...
Altvater-Hughes TE, Hodgins HP, Hodgins DC, Bauman CA, Mallard BA.   +4 more
europepmc   +3 more sources

High-Throughput Sequencing of Complementarity Determining Region 3 in the Heavy Chain of B-Cell Receptor in Renal Transplant Recipients: A Preliminary Report. [PDF]

J Clin Med, 2022
Background: Graft failure resulting from rejection or any other adverse event usually originates from an aberrant and/or exaggerated immune response and is often catastrophic in renal transplantation. So, it is essential to monitor patients’ immune status for detecting a rejection/graft failure early on.
Wu TH, Liao HT, Li TH, Tsai HC, Lin NC, Chen CY, Tsai SF, Huang TH, Tsai CY, Yu CL.   +9 more
europepmc   +3 more sources

Humanization of antibodies using heavy chain complementarity-determining region 3 grafting coupled with in vitro somatic hypermutation. [PDF]

J Biol Chem, 2013
A method for simultaneous humanization and affinity maturation of monoclonal antibodies has been developed using heavy chain complementarity-determining region (CDR) 3 grafting combined with somatic hypermutation in vitro. To minimize the amount of murine antibody-derived antibody sequence used during humanization, only the CDR3 region from a murine ...
Bowers PM, Neben TY, Tomlinson GL, Dalton JL, Altobell L, Zhang X, Macomber JL, Wu BF, Toobian RM, McConnell AD, Verdino P, Chau B, Horlick RA, King DJ.   +13 more
europepmc   +4 more sources

Characteristics Peripheral Blood IgG and IgM Heavy Chain Complementarity Determining Region 3 Repertoire before and after Immunization with Recombinant HBV Vaccine. [PDF]

PLoS ONE, 2017
Immunization with recombinant HBV vaccine induces specific immune responses in human causing B lymphocytes to produce protective HBsAb, and to form memory B lymphocytes, thereby facilitating HBV immunity in the body.
Long Ma, Xiaomei Wang, Xiaoying Bi, Jiezuan Yang, Bin Shi, Xiaoyan He, Rui Ma, Qingqing Ma, Xinsheng Yao   +8 more
doaj   +2 more sources

Alanine Mutagenesis in the Complementarity Determining Region 3 of the MTB and HIV-1 Peptide-Bispecific T Cell Receptor Beta Chain Affects Ligand Recognition [PDF]

Frontiers in Immunology, 2017
Mycobacterium tuberculosis/human immunodeficiency virus (MTB/HIV) coinfection presents a special challenge to the prevention and treatment of tuberculosis and HIV/AIDS.
Chao-Ying Zhou, Rui-Ning Wang, Qian Wen, Wen-Ting He, Shi-Meng Zhang, Xia-Lin Du, Jia-Hui Yang, Li Ma   +7 more
doaj   +2 more sources

Length of the antibody heavy chain complementarity determining region 3 as a specificity-determining factor

Journal of Molecular Recognition, 2004
AbstractThe antigen binding site of an antibody is made up of residues residing in six hypervariable loops of the heavy and light chains. In most cases several or all of these loops are required for the establishment of the antigen‐binding surface. Five of these loops display a limited diversity in length and sequence while the third complementarity ...
Yvelise Barrios, Mats Ohlin
exaly   +4 more sources