Results 41 to 50 of about 243,069 (278)

Microtubules in Bacteria: Ancient Tubulins Build a Five-Protofilament Homolog of the Eukaryotic Cytoskeleton [PDF]

, 2011
Microtubules play crucial roles in cytokinesis, transport, and motility, and are therefore superb targets for anti-cancer drugs. All tubulins evolved from a common ancestor they share with the distantly related bacterial cell division protein FtsZ, but ...
A Briegel, A Marchler-Bauer, A. J Koster, A. J Martin-Galiano, Alasdair W. McDowall, B Miroux, C Jenkins, C Suloway, C. A Sontag, C. A Sontag, C. H Aylett, C. V Iancu, D Bermudes, D Nicastro, D Schlieper, D. N Mastronarde, D. N Mastronarde, D. P Devos, E Nogales, E Nogales, E Nogales, E Nogales, E. F Pettersen, E. G Reynaud, E. I Tocheva, F Amat, Giulio Petroni, Grant J. Jensen, H Erickson, H Sui, H. P Erickson, J Cope, J Lowe, J. B Heymann, J. D Thompson, J. D Wisotzkey, J. O McInerney, J. R McIntosh, K. C Lee, K. S Makarova, K. T Tokuyasu, K. T Tokuyasu, L Gan, L Ni, L. A Amos, L. A Amos, Linda Amos, M Pilhofer, M Pilhofer, M Pilhofer, M Takeda, M Wagner, M. S Ladinsky, M. S Ladinsky, Mark S. Ladinsky, Martin Pilhofer, P Forterre, P. J Keeling, R. I Koning, S Vaughan, S. K Dutcher, S. Q Zheng, T Muller, T. K Attwood, W Ludwig, W. F Tivol, Z Li   +66 more
core   +5 more sources

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco [PDF]

BMC Research Notes, 2016
A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries.In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV
Fellahi, Siham, Harrak, Mehdi El, Kuhn, Jens H., Sebbar, Ghizlane, Bouaiti, El Arbi, Khataby, Khadija, Fihri, Ouafae Fassi, Houadfi, Mohammed El, Ennaji, My Mustapha   +8 more
openaire   +2 more sources

First report of canine Astrovirus and Sapovirus in Greece, hosting both asymptomatic and gastroenteritis symptomatic dogs

Virology Journal, 2022
Background Astrovirus, Norovirus and Sapovirus are widely distributed viruses in humans and animals worldwide. They have frequently been associated with disease, mainly of gastroenteric nature.
Efthymia Stamelou, Ioannis A. Giantsis, Konstantinos V. Papageorgiou, Evanthia Petridou, Irit Davidson, Zoe S. Polizopοulou, Anna Papa, Spyridon K. Kritas   +7 more
doaj   +1 more source

Microfluidic DNA amplification - a review [PDF]

, 2009
The application of microfluidic devices for DNA amplification has recently been extensively studied. Here, we review the important development of microfluidic polymerase chain reaction (PCR) devices and discuss the underlying physical principles for the ...
Ozdemir, Pinar, Zhang, Yonghao
core   +1 more source

MOLECULAR DETECTION OF NEPOVIRUSES IN PAKISTANI GRAPEVINE GERMPLASM USING REAL TIME qRT-PCR AND CONVENTIONAL RT-PCR [PDF]

The Journal of Animal and Plant Sciences, 2021
The aim of the present work was to investigate the molecular identification and characterization of the three different viruses including Grapevine Fanleaf virus (GFLV), Arabis Mosaic virus (ArMV) and Tobacco ringspot virus (TRSV) from Nepovirus group in the main grape producing regions of Pakistan by using reverse transcription polymerase reaction (RT-
A Younas, S Naz
openaire   +1 more source

FLT3 Length Mutations as Marker for Follow-Up Studies in Acute Myeloid Leukaemia [PDF]

, 2004
Length mutations within the FLT3 gene (FLT3-LM) can be found in 23% of acute myeloid leukaemia (AML) and thus are the most frequent mutations in AML. FLT3-LM are highly correlated with AML with normal karyotype and other cytogenetic aberrations of the ...
Haferlach, Torsten, Hiddemann, Wolfgang, Kern, Wolfgang, Schnittger, Susanne, Schoch, Claudia   +4 more
core   +1 more source

Analytical sensitivity comparison of 14 conventional and three rapid RT-PCR assays for SARS-CoV-2 detection

Journal of Virological Methods, 2021
Recent reports have compared the analytical sensitivities of some SARS-CoV-2 RT-PCR assays, but differences in the viral materials used for these evaluations made comprehensive conclusions difficult. We carried out a direct comparison of the analytical sensitivities of 14 conventional and three rapid RT-PCR assays for the detection of SARS-CoV-2.
Wang, Xueliang, Huang, Zhongqiang, Song, Jian, Xiao, Yanqun, Wang, Hualiang   +4 more
openaire   +2 more sources

Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]

, 2020
Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P, Ibrahim, AEC, Ma, J, Van Dolleweerd, CJ   +3 more
core   +1 more source

Missed detections of influenza A(H1)pdm09 by real-time RT–PCR assay due to haemagglutinin sequence mutation, December 2017 to March 2018, northern Viet Nam

Western Pacific Surveillance and Response, 2019
Introduction: There are two methods of reverse transcription polymerase chain reaction (RT–PCR) that have been the common methods to detect influenza infections: conventional and real-time RT–PCR.
Hoang Vu Mai Phuong, Ung Thi Hong Trang, Nguyen Le Khanh Hang, Nguyen Thanh Thuy, Le Thi Thanh, Nguyen Vu Son, Nguyen Phuong Anh, Tran Thi Thu Huong, Vuong Duc Cuong, Le Quynh Mai   +9 more
doaj   +1 more source

Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus

Molecular and Cellular Probes, 2017
Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV) is a highly contagious intestinal disease, resulting in substantial economic losses to the swine industry worldwide. In this study, three assays, namely a conventional reverse transcription-polymerase chain reaction (RT-PCR), a SYBR Green I real-time RT-PCR and a TaqMan ...
Xinrong, Zhou, Tiansheng, Zhang, Deping, Song, Tao, Huang, Qi, Peng, Yanjun, Chen, Anqi, Li, Fanfan, Zhang, Qiong, Wu, Yu, Ye, Yuxin, Tang   +10 more
openaire   +2 more sources