Potential capacity of interferon-α to eliminate covalently closed circular DNA (cccDNA) in hepatocytes infected with hepatitis B virus [PDF]
Gut Pathogens, 2021 Interferon-alpha (IFN-α) and nucleot(s)ide analogs (NAs) are first-line drugs for the treatment of chronic hepatitis B virus (HBV) infections. Generally, NAs target the reverse transcription of HBV pregenomic RNA, but they cannot eliminate covalently ...
Gang Wang +10 more
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Detection of the hepatitis B virus (HBV) covalently-closed-circular DNA (cccDNA) in mice transduced with a recombinant AAV-HBV vector [PDF]
Antiviral Research, 2017 Hepatitis B Virus (HBV) persists in infected hepatocytes as an episomal covalently-closed-circular DNA mini-chromosome, called cccDNA. As the main nuclear transcription template, HBV cccDNA is a key replication intermediate in the viral life cycle. Little is known about the mechanisms involved in its formation, maintenance and fate under antiviral ...
Barbara Testoni +2 more
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Frontiers in Cellular and Infection MicrobiologyChronic hepatitis B virus (HBV) infection continues to be a global health concern because current treatments such as interferon-α and nucleos(t)ide analogs cannot fully eliminate the virus due to persistence of covalently closed circular DNA (cccDNA) and integrated HBV DNA.
Kyongmin Kim, Sun Park
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New Insights on Molecular Mechanism of Hepatitis B Virus Covalently Closed Circular DNA Formation [PDF]
Cells, 2020 The chronic factor of the Hepatitis B Virus (HBV), specifically the covalently closed circular DNA (cccDNA), is a highly stable and active viral episomal genome established in the livers of chronic hepatitis B patients as a constant source of disease ...
Alexander L. Marchetti, Haitao Guo
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DOCK11 promotes HBV cccDNA formation through a PARP1-dependent mechanism [PDF]
Microbiology SpectrumHepatitis B virus (HBV) infection remains a major global health concern due to the persistence of covalently closed circular DNA (cccDNA) in the hepatocyte nucleus, which contributes to viral persistence and hepatocarcinogenesis despite antiviral therapy.
Hideo Takayama +11 more
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Circular nucleic acids at the host-virus interface: from immune modulation to therapeutic innovation [PDF]
Frontiers in ImmunologyNucleic acids, long regarded as linear polymers, are now recognized to also exist in circular forms with profound biological significance in both eukaryotic hosts and viruses.
Jiacheng Lou +3 more
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Dual HBV cccDNA-linked HiBiT reporter hepatocyte models for screening of candidate cccDNA modulators [PDF]
VirulenceChronic hepatitis B remains difficult to cure because the viral covalently closed circular DNA (cccDNA) minichromosome can persist and sustain viral transcription, creating a need for scalable, reporter readouts that facilitate early discovery of cccDNA ...
Jiali Cao +13 more
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Hepatitis B virus cccDNA is formed through distinct repair processes of each strand
Nature Communications, 2021 HBV covalently closed circular DNA (cccDNA) enables and persists in chronic infection, but the molecular mechanism of its formation is unclear. Here, Wei and Ploss elucidate the detailed kinetics and biochemical steps by which the relaxed circular DNA is
Lei Wei, Alexander Ploss
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Early Steps of Hepatitis B Life Cycle: From Capsid Nuclear Import to cccDNA Formation
Viruses, 2021 Hepatitis B virus (HBV) remains a major public health concern, with more than 250 million chronically infected people who are at high risk of developing liver diseases, including cirrhosis and hepatocellular carcinoma.
João Diogo Dias +2 more
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Emerging Microbes and Infections, 2021 Upon infection of hepatocyte, Hepatitis B virus (HBV) genomic DNA in nucleocapsid is transported into the nucleus and converted into a covalently closed circular (ccc) DNA to serve as the template for transcription of viral RNAs.
Jin Hu +7 more
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