Results 151 to 160 of about 1,216 (181)

Recombinant expression of crotoxin and comparison to native crotoxin as immunogens for anti-crotalid antivenoms

Protein Expression and Purification
The crotoxin complex is identified as the primary lethal toxin in various species of rattlesnakes. It comprises two subunits: CtxA, a non-toxic protein, and CtxB, a minimally toxic enzymatic phospholipase A2. These components exert neurotoxic effects by disrupting neuromuscular transmission, specifically by inhibiting the release of acetylcholine ...
Miguel Angel Mejía Sánchez, Samuel Cardoso Arenas, Ricardo Miranda Blancas, Herlinda Clement, Ligia Luz Corrales García, Adrián Marcelo Franco-Vásquez, Ivan Arenas, Alejandro Carbajal Saucedo, Gerardo Corzo   +8 more
openaire   +2 more sources

Induction of tolerance to crotoxin in mice.

The Journal of Pharmacology and Experimental Therapeutics, 1993
Crotoxin, the major toxic component from the venom of Crotalus durissus terrificus is a potent neurotoxin (LD50, i.p., mice, 0.09 mg/kg) which possesses phospholipase A2 activity and causes a blockade of neuromuscular transmission. In this article, we show that mice injected daily with progressively increasing doses of crotoxin develop tolerance to the
M, Okamoto, L J, Viskatis, G, de la Roza, J C, Vidal   +3 more
openaire   +2 more sources

Systemic skeletal muscle necrosis induced by crotoxin

Toxicon, 2001
Systemic skeletal muscle necrosis induced by crotoxin, the major component of the venom of Crotalus durissus terrificus, was investigated. Mice received an intramuscular injection of crotoxin (0.35mg/kg body weight) into the right tibialis anterior (TA) muscles, which were evaluated 3h, 24h and 3 days later.
T F, Salvini, A C, Amaral, E H, Miyabara, J A, Turri, P M, Danella, H S, Selistre de Araújo   +5 more
openaire   +2 more sources

Effects of crotoxin on the isolated guinea pig heart

Toxicon, 1990
The effects of crotoxin, isolated from the venom of the South American rattlesnake, Crotalus durissus terrificus, were investigated on isolated guinea pig hearts, perfused with Locke solution, by the Langendorff method. The cardiac beats and the electrocardiogram were simultaneously registered and the creatine kinase (CK) activity of the perfusate ...
P E, Santos, S D, Souza, L, Freire-Maia, A P, Almeida   +3 more
openaire   +2 more sources

Investigations on the mechanism of action of crotoxin.

Journal de physiologie, 1985
Crotoxin, the major toxic protein of Crotalus durissus terrificus, is composed of a basic phospholipase, component-B, and of an acidic subunit, component-A. The crotoxin complex is insensitive to an active site directed reagent, p-bromophenacyl bromide, while its isolated enzymatic component-B is rapidly and irreversibly inactivated.
F, Radvanyi, C, Bon
openaire   +2 more sources

Muscle necrosis caused by the sub-units of crotoxin

Toxicon, 1986
The effects of the basic (CB) and acidic (CA) subunits of crotoxin on rat skeletal muscle in vivo have been studied. CB caused muscle necrosis, with damage limited to an outer rim of muscle fibres in rat soleus. The inflammatory response was relatively small.
J A, Kouyoumdjian, J B, Harris, M A, Johnson   +2 more
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Effects of subunit cross-linking on the properties of crotoxin

Toxicon, 1990
Crotoxin was cross-linked using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride. Cross-linked crotoxin had the expected amino-terminal amino acids, amino acid composition, behavior on SDS-PAGE and an 80% reduction of reactable lysine residues.
B W, Lennon, D, Plummer, I I, Kaiser
openaire   +2 more sources

Postsynaptic Effects of Crotoxin and of Its Isolated Subunits

European Journal of Biochemistry, 1979
Crotoxin is a potent neurotoxin from the venom of Crotalus durissus terrificus. It is composed of two subunits: a basic phospholipase A2 with low toxicity (component B) and an acidic protein seemingly devoid of intrinsic biological activity (component A).
C, Bon, J P, Changeux, T W, Jeng, H, Fraenkel-Conrat   +3 more
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Crotoxin

Nature, 1939
K. H. SLOTTA, H. FRAENKEL-CONRAT
openaire   +1 more source

BIOLOGICAL ACTIVITIES OF CROTOXIN AND AMINO ACID SEQUENCE OF CROTOXIN B

1980
H. Fraenkel-Conrat, T-W. Jeng, M. Hsiang
openaire   +1 more source