Results 91 to 100 of about 2,441,948 (283)

Cryo-electron tomography for the structural study of mitochondrial translation

, 2019
Cryo-electron tomography (cryo-ET) enables the three-dimensional (3D) structural characterization of macromolecular complexes in their physiological environment.
Englmeier, Robert, Förster, Friedrich, Cryo-EM, Sub Cryo - EM   +3 more
core  

Immersion Freezing of Cell Monolayers for Cryo-Electron Tomography

, 2011
INTRODUCTIONCryo-transmission electron microscopy (cryo-TEM) is not limited to the visualization of suspended macromolecules in their native hydrated state. It can also be used to elucidate the molecular architecture of peripheral parts of adherent cells:
Marlene Brandstetter, Veronika I. Wonesch, Guenter P. Resch, Edit Urban   +3 more
core   +1 more source

Localize.pytom: a modern webserver for cryo-electron tomography [PDF]

Nucleic Acids Research, 2015
Localize.pytom, available through http://localize.pytom.org is a webserver for the localize module in the PyTom package. It is a free website and open to all users and there is no login requirement. The server accepts tomograms as they are imaged and reconstructed by Cryo-Electron Tomography (CET) and returns densities and coordinates of candidate ...
openaire   +3 more sources

Differential Sedimentation and Cryo‐TEM Mapping Resolve Structural Heterogeneity in Conjugated Polymer Nanowires for Programmable Stretchable Electronics

Advanced Materials, EarlyView.
Density‐driven differential sedimentation fractionates solution‐assembled P3HT nanowires into crystalline and disordered constituents. High‐resolution cryo‐TEM directly visualizes the molecular chain packing within individual nanowires in real space, and recombining the fractions programs the mobility–stretchability trade‐off without chemical ...
Junyeon Yoon, Jun Ho Hwang, Eunji Lee
wiley   +1 more source

Cryo-ET tilt series for the 3D structure presented in the figures of the paper entitled "Angle Between DNA Linker and Nucleosome Core Particle Regulates Array Compaction by Individual-Particle Cryo-Electron Tomography"

Cryo-ET raw images and tilt series used for the published 3D structures shown in the figures of the paper entitled "Angle Between DNA Linker and Nucleosome Core Particle Regulates Array Compaction by Individual-Particle Cryo-Electron Tomography"
Jianfang Liu (13988585), P.D. Ashby (7343498), Jinhui Tao (18418375), Gang Ren (18417882), Carlos Bustamante (18418381), César-Díaz Celis (13988572), Meng Zhang (13988567)   +6 more
core   +1 more source

Cryo-scanning transmission electron tomography of vitrified cells

, 2014
Cryo-electron tomography (CET) of fully hydrated, vitrified biological specimens has emerged as a vital tool for biological research. For cellular studies, the conventional imaging modality of transmission electron microscopy places stringent constraints
Michael Elbaum, Houben, Lothar, Elbaum, Michael, Wolf, Sharon Grayer, Sharon Grayer Wolf, Lothar Houben   +5 more
core   +1 more source

Electron Tomography and Correlative Approaches in Platelet Studies [PDF]

, 2018
Blood platelets play a central role in the arrest of bleeding and the development of thrombosis. Unraveling the complex processes of platelet biogenesis from megakaryocytes, platelet adhesion, aggregation, and secretory responses are important topics in ...
Geerts, Willie J C, Kasia B. Engberts, Heijnen, Harry F G, Willie J. C. Geerts, Seinen, Cor, Engberts, Kasia B, Harry F. G. Heijnen, Cor Seinen, Crystal and Structural Chemistry, Cryo-EM, Sub Cryo - EM   +10 more
core   +1 more source

Increasing Lipid Nanoparticle mRNA Copy Number Improves Transfection Potency

Advanced Materials, EarlyView.
As lipid nanoparticle (LNP) systems are the leading platform for in vivo nucleic acid delivery, improving transfection potency of these systems is of considerable interest. In this work, we explored a post‐mixing formulation method involving a two‐step buffer exchange that increased the number of mRNA copies within each LNP while reducing the number of
Yao Zhang, Jerry Leung, Judy Cheng, Arash Momeni, Albert Kamanzi, Ariadne Tuckmantel Bido, Suiyang Liao, Emma Kang, Colton Strong, Christian J. Kastrup, Sabrina Leslie, Miffy Hok Yan Cheng, Pieter R. Cullis   +12 more
wiley   +1 more source

CEMOVIS; cryo-electron microscopy of vitreous sections

, 2008
Institut für AnatomieNotes: 2008016244 GBA7A2002 edited by Annie Cavalier, Danièle Spehner, Bruno M. Humbel. ill. ; 26 cm. Includes bibliographical references and index. Slam-freezing, metal-mirror freezing / Danièle Spehner and Ludwig Edelmann -- Plunge-
Al-Amoudi, A., Dubochet, J., Bouchet-Marquis, C., Zuber, Benoît, Eltsov, M.   +4 more
core  

3D reconstruction of HIV virus-like particles from cryo-electron tomography data

, 2022
3D reconstruction of HIV virus-like particles from cryo-electron tomography data in EMPIAR-10164 and associated particle ...
Alister Burt
core   +1 more source