Results 81 to 90 of about 2,441,948 (283)

In Situ Interfacial Assembly of Dynamic Semi‐Interpenetrating Biopolymer Networks for Robust and Mechanically Adaptive Droplet Interfaces

Advanced Functional Materials, EarlyView.
A dynamic interfacial semi‐interpenetrating polymer network is constructed by sequential assembly of β‐sheet‐rich SF and CS‐RL. The SF framework provides mechanical strength, while dynamically associated CS‐RL enables local rearrangement, allowing droplets to rapidly reconfigure without loss of interfacial integrity.
Yaping Zhang, Yujie Liu, Yikai Xu, Xunda Feng, Ling Zhang, Hongbo Zeng, Hao Liu, Xiaohui Mao   +7 more
wiley   +1 more source

Purification and Cryo-Electron Microscopy Analysis of Bacterial Appendages

Bio-Protocol
A number of extracellular helical protein polymers are crucial for supporting bacterial motility. The bacterial flagellum is a polymeric appendage used to support cellular motility.
Juan Sanchez, Joseph Baumgardt, Elizabeth Wright   +2 more
doaj   +1 more source

Developments in cryo-electron tomography for in situ structural analysis

, 2015
Structural analysis of macromolecular assemblies and their remodeling during physiological processes is instrumental to defining the fundament of cellular and molecular biology.
Sorrentino, Simona, Medalia, Ohad, Harapin, Jan, Sapra, K Tanuj, Dubrovsky, Anna, Dubrovsky A, Sapra KT, Medalia O, Harapin J, Sorrentino S   +9 more
core   +1 more source

Advanced cryo-tomography workflow developments - correlative microscopy, milling automation and cryo-lift-out [PDF]

, 2020
Cryo-electron tomography (cryo-ET) is a groundbreaking technology for 3D visualisation and analysis of biomolecules in the context of cellular structures. It allows structural investigations of single proteins as well as their spatial arrangements within
Erdmann, P., Goetze, B., Kuba, J., Rigort, A., Berka, L., Kirmse, R., Mitchels, J., De Bock, J., Hovorka, M., König, J.   +9 more
core   +1 more source

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

Advanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj, Xilal Y. Rima, Kim T. Nguyen, Courtney Culkins, Nana Boateng, Jacob Doon‐Ralls, Alejandro Bresolin, Xin Huang, Hong Li, Vahedi Amid, Ajeet Pal Singh, Dharti Shantaram, Anastasiia Amari, Mangesh D. Hade, Nicholas R. Merriam, Zachary D. Schultz, Scott Q. Harper, Nizar Y. Saad, Setty M. Magaña, Willa A. Hsueh, Rachel E. Kopec, Blaise R. Kimmel, Andre F. Palmer, Eduardo Reátegui   +23 more
wiley   +1 more source

Frontiers in integrative structural modeling of macromolecular assemblies

QRB Discovery
Integrative modeling enables structure determination for large macromolecular assemblies by combining data from multiple experiments with theoretical and computational predictions.
Kartik Majila, Shreyas Arvindekar, Muskaan Jindal, Shruthi Viswanath   +3 more
doaj   +1 more source

Purification of Herpesvirus Virions and Capsids

Bio-Protocol, 2014
This protocol was designed for large-scale purification of herpesvirus particles by cell culture. Virions and capsids are isolated from extracellular culture media and cell nuclei, respectively.
Xinghong Dai, Z. Zhou
doaj   +1 more source

Correlated cryogenic photoactivated localization microscopy and cryo-electron tomography

, 2014
Cryo-electron tomography (CET) produces three-dimensional images of cells in a near-native state at macromolecular resolution, but identifying structures of interest can be challenging. Here we describe a correlated cryo-PALM (photoactivated localization
Songye Chen, Jensen, G., Treuner-Lange, A., Anke Treuner-Lange, Yi-Wei Chang, Chang, Y., Elitza I Tocheva, Stephanie Löbach, Grant J Jensen, Chen, S., Sogaard-Andersen, L., Lotte Søgaard-Andersen, Tocheva, E., Loebach, S.   +13 more
core   +1 more source

Towards Temporal Resolution in Correlative Cryo-electron Tomography

, 2023
Cryo-electron tomography (cryo-ET) has given unprecedented insights into the structure of macromolecules in their native cellular environment. This technique bridges the gap between high-resolution ex-situ single particle analysis and low-resolution, in ...
Brenner, J. ; https://orcid.org/, Plitzko, J., Wilfling, F. ; https://orcid.org/, Klumpe, S.   +3 more
core   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source