Results 51 to 60 of about 2,441,948 (283)
Cryo-electron tomography to study viral infection
Biochemical Society Transactions, 2023 Developments in cryo-electron microscopy (cryo-EM) have been interwoven with the study of viruses ever since its first applications to biological systems. Following the success of single particle cryo-EM in the last decade, cryo-electron tomography (cryo-ET) is now rapidly maturing as a technology and catalysing great advancement in structural virology
Miles Graham, Peijun Zhang
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Molecular Oncology, EarlyView.Extracellular vesicle (EV) lipidomic profiling of plasma and ascites from patients with high‐grade serous ovarian cancer and of ovarian cancer cell lines reveals enrichment of triglycerides (TGs), diacylglycerols (DGs), phosphatidylcholine (PCs), sphingomyelins (SMs), reflecting tumor metabolic reprogramming.
Shikha Rani +9 more
wiley +1 more source
Zooming in and out: Exploring RNA Viral Infections with Multiscale Microscopic Methods
VirusesRNA viruses, being submicroscopic organisms, have intriguing biological makeups and substantially impact human health. Microscopic methods have been utilized for studying RNA viruses at a variety of scales.
Cheng-An Lyu, Yao Shen, Peijun Zhang
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Cryo-electron tomography of bacterial viruses
Virology, 2013 Bacteriophage particles contain both simple and complex macromolecular assemblages and machines that enable them to regulate the infection process under diverse environmental conditions with a broad range of bacterial hosts. Recent developments in cryo-electron tomography (cryo-ET) make it possible to observe the interactions of bacteriophages with ...
Guerrero-Ferreira, Ricardo C. +1 more
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Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation
Molecular Oncology, EarlyView.How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim +11 more
wiley +1 more source
Cryo-CLXEM introduces cryo-SXT to bridge the resolution gap in cryo-CLEM
Communications BiologyCryo-imaging in cellular biology provides the means to visualize the cellular interior at close-to-native conditions. A cornerstone in the field has been cryo-correlative light and electron microscopy (cryo-CLEM), with cryo-visible light fluorescent ...
Johannes Groen +7 more
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The resolution revolution revisited
IUCrJIn this issue of IUCrJ, Subramaniam, Kühlbrandt & Henderson present an overview of the remarkable progress that has been made in electron cryo-microscopy and electron cryo-tomography.
Edward H. Egelman
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FEBS Open Bio, EarlyView.The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
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Unraveling atomic complexity from frozen samples
Structural DynamicsCryo-electron microscopy (cryo-EM) is a significant driver of recent advances in structural biology. Cryo-EM is comprised of several distinct and complementary methods, which include single particle analysis, cryo-electron tomography, and microcrystal ...
Yasmeen N. Ruma +2 more
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Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy
eLife, 2021 Keratin intermediate filaments are an essential and major component of the cytoskeleton in epithelial cells. They form a stable yet dynamic filamentous network extending from the nucleus to the cell periphery, which provides resistance to mechanical ...
Miriam S Weber +5 more
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