Results 71 to 80 of about 23,786 (199)

Strategies and mechanisms of precision genome engineering: From gene editing to genome writing

iMetaOmics, EarlyView.
In this review, we examined the progression of genome manipulation from stochastic nuclease‐mediated cutting toward precise editing and programmable genome writing. We discussed tools like multi‐kilobase RNA‐guided integrators and Artificial Intelligence (AI)‐designed effectors and showed how these advances enable researchers to treat genomes as ...
Kerui Huang, Jianhong Tian, Wenyan Zhao, Siqin Zhang, Lixuan Xiang, Meiqing Yang, Lu Liu, Yazhou Huang, Ningyun Zhang, Lei Sun, Peng Xie, Nanyu Kuang, Hong Zhou, Chengfeng Qiu, Jun Yan, Xiaocheng Jiang, Qiang Tu, Yun Wang, Haoliang Hu, Youming Zhang   +19 more
wiley   +1 more source

An alternative CTCF isoform antagonizes canonical CTCF occupancy and changes chromatin architecture to promote apoptosis [PDF]

Nature Communications, 2019
AbstractCTCF plays key roles in gene regulation, chromatin insulation, imprinting, X chromosome inactivation and organizing the higher-order chromatin architecture of mammalian genomes. Previous studies have mainly focused on the roles of the canonical CTCF isoform.
Jiao Li, Kaimeng Huang, Gongcheng Hu, Isaac A. Babarinde, Yaoyi Li, Xiaotao Dong, Yu-Sheng Chen, Liping Shang, Wenjing Guo, Junwei Wang, Zhaoming Chen, Andrew P. Hutchins, Yun-Gui Yang, Hongjie Yao   +13 more
openaire   +3 more sources

Exploring Interactive Effects of Arsenic and Selenium Exposure on Larval Zebrafish (Danio rerio)

Environmental Toxicology, EarlyView.
ABSTRACT Arsenic contamination in aquatic ecosystems is a major environmental concern. Selenium (Se) helps mitigate oxidative stress and hence could reduce the toxic effects of arsenic. However, Se can also be toxic at high concentrations. Given that these metalloids commonly co‐occur in the environment, there is a pressing need for further research ...
Owen Luo, Jinnath Rehana Ritu, Md Helal Uddin, Sravan Kumar Putnala, Mahesh Rachamalla, Som Niyogi, Douglas P. Chivers   +6 more
wiley   +1 more source

ADP-ribose polymers localized on Ctcf–Parp1–Dnmt1 complex prevent methylation of Ctcf target sites [PDF]

Biochemical Journal, 2011
PARylation [poly(ADP-ribosyl)ation] is involved in the maintenance of genomic methylation patterns through its control of Dnmt1 [DNA (cytosine-5)-methyltransferase 1] activity. Our previous findings indicated that Ctcf (CCCTC-binding factor) may be an important player in key events whereby PARylation controls the unmethylated status of some CpG-rich ...
ZAMPIERI, Michele, GUASTAFIERRO, Tiziana, CALABRESE, ROBERTA, CICCARONE, FABIO, BACALINI, MARIA GIULIA, REALE, Anna, Perilli M., Passananti C., CAIAFA, Paola   +8 more
openaire   +9 more sources

Exploring the changing landscape of cell-to-cell variation after CTCF knockdown via single cell RNA-seq

BMC Genomics, 2019
Background CCCTC-Binding Factor (CTCF), also known as 11-zinc finger protein, participates in many cellular processes, including insulator activity, transcriptional regulation and organization of chromatin architecture.
Wei Wang, Gang Ren, Ni Hong, Wenfei Jin
doaj   +1 more source

CGGBP1-regulated cytosine methylation at CTCF-binding motifs resists stochasticity

BMC Genetics, 2020
Background The human CGGBP1 binds to GC-rich regions and interspersed repeats, maintains homeostasis of stochastic cytosine methylation and determines DNA-binding of CTCF.
Manthan Patel, Divyesh Patel, Subhamoy Datta, Umashankar Singh   +3 more
doaj   +1 more source

The Functional Role and Molecular Characterization of the Therapeutic Target CLDN6 in Germ Cell Tumors

Andrology, EarlyView.
ABSTRACT Background The tight junction protein CLDN6 has been identified as a cancer‐associated cell surface marker that is rarely expressed in healthy tissues. In testicular germ cell tumors (GCT), CLDN6 is particularly detectable in seminomas, embryonal carcinomas, and choriocarcinomas.
Jule Zwick, Margaretha A. Skowron, Gereon Poschmann, Patrick Petzsch, Ann Kathrin Bergmann, Kai Stühler, Jochen Hecht, Daniel Nettersheim   +7 more
wiley   +1 more source

MEN1 Deficiency Drives Lung Cancer Progression via Activation of MMP10‐Mediated Angiogenesis

Cancer Science, EarlyView.
MEN1 deficiency promotes JunD‐mediated upregulation of MMP10 expression, which cleaves pro‐HB‐EGF and activates endothelial EGFR. This triggers the PI3K/Akt and MEK/ERK signaling pathways, thereby driving angiogenesis and tumor progression. MMP10 inhibitor treatment blocks this cleavage, suppresses EGFR signaling, and thereby delays malignant ...
Chengyu Wei, Xiangyu Liu, Shimin Hu, Xiaodie Shen, Yingli Liu, Xinxin Qin, Ye Zhou, Jianqi Zheng, Bingke Tian, Jingfang Hong, Yongxia Song, Di Wang, Linlin Peng, Jinxia Ding, Fang Wang, Hui Zhang, Shihao Zhang, Huan Qiu   +17 more
wiley   +1 more source

DNA methylation insulates genic regions from CTCF loops near nuclear speckles

eLife
The insulator protein CTCF is essential for mediating chromatin loops and regulating gene expression. While it is established that DNA methylation hinders CTCF binding, the impacts of this methylation-sensitive CTCF binding on chromatin architecture and ...
Shelby A Roseman, Allison P Siegenfeld, Ceejay Lee, Nicholas Z Lue, Amanda L Waterbury, Brian B Liau   +5 more
doaj   +1 more source

TET-Catalyzed 5-Carboxylcytosine Promotes CTCF Binding to Suboptimal Sequences Genome-wide

iScience, 2019
Summary: The mechanisms supporting dynamic regulation of CTCF-binding sites remain poorly understood. Here we describe the TET-catalyzed 5-methylcytosine derivative, 5-carboxylcytosine (5caC), as a factor driving new CTCF binding within genomic DNA ...
Kyster K. Nanan, David M. Sturgill, Maria F. Prigge, Morgan Thenoz, Allissa A. Dillman, Mariana D. Mandler, Shalini Oberdoerffer   +6 more
doaj   +1 more source