Cyclin A triggers Mitosis either via the Greatwall kinase pathway or Cyclin B. [PDF]
EMBO J, 2020 Two mitotic cyclin types, cyclin A and B, exist in higher eukaryotes, but their specialised functions in mitosis are incompletely understood. Using degron tags for rapid inducible protein removal, we analyse how acute depletion of these proteins affects ...
Hégarat N +13 more
europepmc +16 more sources
Ordered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit [PDF]
eLife, 2020 APC/C-mediated proteolysis of cyclin B and securin promotes anaphase entry, inactivating CDK1 and permitting chromosome segregation, respectively. Reduction of CDK1 activity relieves inhibition of the CDK1-counteracting phosphatases PP1 and PP2A-B55 ...
James Holder +2 more
doaj +3 more sources
Cyclin B Translation Depends on mTOR Activity after Fertilization in Sea Urchin Embryos. [PDF]
PLoS ONE, 2016 The cyclin B/CDK1 complex is a key regulator of mitotic entry. Using PP242, a specific ATP-competitive inhibitor of mTOR kinase, we provide evidence that the mTOR signalling pathway controls cyclin B mRNA translation following fertilization in ...
Héloïse Chassé +5 more
doaj +3 more sources
The 3' untranslated region of the cyclin B mRNA is not sufficient to enhance the synthesis of cyclin B during a mitotic block in human cells. [PDF]
PLoS ONE, 2013 Antimitotic agents are frequently used to treat solid tumors and hematologic malignancies. However, one major limitation of antimitotic approaches is mitotic slippage, which is driven by slow degradation of cyclin B during a mitotic block.
Dominik Schnerch +4 more
doaj +7 more sources
PP1 promotes cyclin B destruction and the metaphase-anaphase transition by dephosphorylating CDC20. [PDF]
Mol Biol Cell, 2020 Ubiquitin-dependent proteolysis of cyclin B and securin initiates sister chromatid segregation and anaphase. The anaphase promoting complex/cyclosome (APC/C) and its co-activator CDC20 form the main ubiquitin E3 ligase for these proteins.
Bancroft J +6 more
europepmc +2 more sources
The effect of vitrification after warming on the expressions of p38, CDK1, and cyclin B in immature goat oocytes followed by in vitro maturation [PDF]
Veterinary World, 2020 Background and Aim: The combination of vitrification techniques and in vitro maturation can reduce oocyte competence. Mitogen-activated protein kinase and maturation-promoting factor are significant in oocyte meiotic maturation regulation.
A. A. Muhammad Nur Kasman +2 more
doaj +2 more sources
Nuclear Export of Cyclin B Mediated by the Nup62 Complex Is Required for Meiotic Initiation in Drosophila Males [PDF]
Cells, 2020 Background: The central channel of the nuclear pore complex plays an important role in the selective transport of proteins between the nucleus and cytoplasm.
Ryotaro Okazaki +2 more
doaj +2 more sources
SGK phosphorylates Cdc25 and Myt1 to trigger cyclin B-Cdk1 activation at the meiotic G2/M transition. [PDF]
J Cell Biol, 2019 The kinase cyclin B-Cdk1 complex is a master regulator of M-phase in both mitosis and meiosis. At the G2/M transition, cyclin B-Cdk1 activation is initiated by a trigger that reverses the balance of activities between Cdc25 and Wee1/Myt1, and is further ...
Hiraoka D +3 more
europepmc +2 more sources
Cyclin B Export to the Cytoplasm via the Nup62 Subcomplex and Subsequent Rapid Nuclear Import Are Required for the Initiation of Drosophila Male Meiosis [PDF]
Cells, 2023 The cyclin-dependent kinase 1 (Cdk1)–cyclin B (CycB) complex plays critical roles in cell-cycle regulation. Before Drosophila male meiosis, CycB is exported from the nucleus to the cytoplasm via the nuclear porin 62kD (Nup62) subcomplex of the nuclear ...
Kanta Yamazoe, Yoshihiro H. Inoue
doaj +2 more sources
Recombinant cyclin B-Cdk1-Suc1 capable of multi-site mitotic phosphorylation in vitro. [PDF]
PLoS ONECyclin-dependent kinase 1 (Cdk1) complexed with cyclin B phosphorylates multiple sites on hundreds of proteins during mitosis. However, it is not fully understood how multi-site mitotic phosphorylation by cyclin B-Cdk1 controls the structures and ...
Keishi Shintomi +4 more
doaj +2 more sources