Results 81 to 90 of about 50,064 (212)

Dynamic allostery governs cyclophilin A–HIV capsid interplay [PDF]

Proceedings of the National Academy of Sciences, 2015
Significance The mechanisms of how Cyclophilin A (CypA) regulates HIV-1 infectivity remain poorly understood. We examined the role of dynamics in capsid (CA) protein assemblies by magic-angle-spinning NMR. The assembled CA is highly dynamic. Dipolar tensors calculated from molecular dynamics trajectories are in quantitative agreement with the
Manman Lu, Guangjin Hou, Huilan Zhang, Christopher L. Suiter, Jinwoo Ahn, In-Ja L. Byeon, Juan R. Perilla, Christopher J. Langmead, Ivan Hung, Peter L. Gor'kov, Zhehong Gan, William Brey, Christopher Aiken, Peijun Zhang, Klaus Schulten, Angela M. Gronenborn, Tatyana Polenova   +16 more
openaire   +2 more sources

Elevated levels of cyclophilin A secreted in milk during bovine mastitis

Journal of Dairy Science
: Bovine mastitis is an inflammatory disease that primarily occurs when bacteria invade and proliferate in the mammary gland, although it can also be caused by physical trauma.
Satoru Takanashi, Kozue Yoshimura, Yuma Endo, Megumi Urakawa, Hidetoshi Sato, Tao Zhuang, Tomohito Hayashi, Yoshio Kiku, Yuya Nagasawa, Haruki Kitazawa, Michael T. Rose, Yoshinobu Uemoto, Kouichi Watanabe, Tomonori Nochi, Hisashi Aso   +14 more
doaj   +1 more source

Thermo‐kinetic analysis space expansion for cyclophilin‐ligand interactions – identification of a new nonpeptide inhibitor using Biacore™ T200

FEBS Open Bio, 2017
We have established a refined methodology for generating surface plasmon resonance sensor surfaces of recombinant his‐tagged human cyclophilin‐A. Our orientation‐specific stabilisation approach captures his‐tagged protein under ‘physiological conditions’
Martin A. Wear, Matthew W. Nowicki, Elizabeth A. Blackburn, Iain W. McNae, Malcolm D. Walkinshaw   +4 more
doaj   +1 more source

MxB sensitivity of HIV-1 is determined by a highly variable and dynamic capsid surface

eLife, 2020
The type one interferon induced restriction factor Myxovirus resistance B (MxB) restricts HIV-1 nuclear entry evidenced by inhibition of 2-LTR but not linear forms of viral DNA.
Richard J Miles, Claire Kerridge, Laura Hilditch, Christopher Monit, David A Jacques, Greg J Towers   +5 more
doaj   +1 more source

Composition of the Schistosoma mansoni worm secretome: Identification of immune modulatory Cyclophilin A. [PDF]

PLoS Neglected Tropical Diseases, 2017
The helminth Schistosoma mansoni modulates the infected host's immune system to facilitate its own survival, by producing excretory/secretory molecules that interact with a variety of the host's cell types including those of the immune system. Herein, we
Achilleas Floudas, Christopher D Cluxton, Julia Fahel, Adnan R Khan, Sean P Saunders, Sylvie Amu, Antonio Alcami, Padraic G Fallon   +7 more
doaj   +1 more source

Sequencing and analysis of the gastrula transcriptome of the brittle star Ophiocoma wendtii [PDF]

, 2012
Background The gastrula stage represents the point in development at which the three primary germ layers diverge. At this point the gene regulatory networks that specify the germ layers are established and the genes that define the differentiated states ...
Garey, James R., Garnhart, Nancy, Livingston, Brian T., Thomas, W. Kelley, Vaughn, Roy   +4 more
core   +2 more sources

A novel role for the peptidyl-prolyl cis-trans isomerase Cyclophilin A in DNA-repair following replication fork stalling via the MRE11-RAD50-NBS1 complex

EMBO Reports
Cyclosporin A (CsA) induces DNA double-strand breaks in LIG4 syndrome fibroblasts, specifically upon transit through S-phase. The basis underlying this has not been described.
Marisa Bedir, Emily Outwin, Rita Colnaghi, Lydia Bassett, Iga Abramowicz, Mark O’Driscoll   +5 more
doaj   +1 more source

A novel secreted cyclophilin-like protein (SCYLP)

Journal of Biological Chemistry, 1991
A novel cyclosporin A binding glycoprotein of 21 kDa was isolated from human milk by several steps of cation exchange chromatography. The corresponding gene was cloned from human T cells, expressed in Escherichia coli and the recombinant protein purified.
G, Spik, B, Haendler, O, Delmas, C, Mariller, M, Chamoux, P, Maes, A, Tartar, J, Montreuil, K, Stedman, H P, Kocher   +9 more
openaire   +2 more sources

Soluble expression of recombinant cyclophilin A in Escherichia coli

浙江大学学报. 农业与生命科学版, 2010
To produce a large amount of soluble cyclophilin A (CypA) protein, the recombinant pRSET -CypA plasmid was transferred into E. coli BL21. Using the 2×YT culture medium, the medium compositions of carbon source, nitrogen source and ampicillin ...
YAN Xiao, XU Li-ren, GUAN Yi-xin, YAO Shan-jing   +3 more
doaj   +1 more source

Localization of cyclophilin A and cyclophilin C mRNA in murine kidney using RT-PCR

Kidney International, 1994
Cyclosporin A (CsA), which is widely used as an immunosuppressant, has a nephrotoxic side effect. The mechanism of this nephrotoxicity is not well understood; however, recent studies suggest that cyclophilin (cyp) is responsible for mediating the immunosuppressive action of CsA through the interaction with the Ca(2+)- and calmodulin-dependent ...
Otsuka, Masakazu, Terada, Yoshio, Yang, Tianxin, Nonoguchi, Hiroshi, Tomita, Kimio, Marumo, Fumiaki   +5 more
openaire   +2 more sources