Results 61 to 70 of about 1,765 (165)

Investigation of Azole Resistance Involving cyp51A and cyp51B Genes in Clinical Aspergillus flavus Isolates

Polish Journal of Microbiology
This study aimed to investigate azole resistance mechanisms in Aspergillus flavus, which involve cyp51A and cyp51B genes. Real-time Reverse Transcriptase qPCR method was applied to determine the overexpression of cyp51A and cyp51B genes for 34 A.
Ghorbel Dhoha, Amouri Imen, Khemekhem Nahed, Neji Sourour, Trabelsi Houaida, Elloumi Moez, Sellami Hayet, Makni Fattouma, Ayadi Ali, Hadrich Ines   +9 more
doaj   +1 more source

Design, Synthesis of Pyridine Based Thiazolidine Derivatives and Investigation of Antimicrobial Activity Targeting Ergosterol Biosynthesis via In Vitro and In Silico Approaches

Chemical Biology &Drug Design, Volume 108, Issue 1, July 2026.
Synthesis of thiazolidine derivatives and investigation of their antimicrobial activities. ABSTRACT Pyridine and thiazolidine ring systems are unique heterocyclic rings since they are present in the structure of various drug/drug‐like molecules and reported in many studies for their distinct biological effects.
Aybüke Züleyha Kaya, Asaf Evrim Evren, Ülküye Dudu Gül, Halide Edip Temel, Leyla Yurttaş   +4 more
wiley   +1 more source

cyp51A mutations, protein modeling, and efflux pump gene expression reveals multifactorial complexity towards understanding Aspergillus section Nigri azole resistance mechanism

Scientific Reports
Black Aspergillus species are the most common etiological agents of otomycosis, and pulmonary aspergillosis. However, limited data is available on their antifungal susceptibility profiles and associated resistance mechanisms.
Pooja Sen, Mukund Vijay, Himanshu Kamboj, Lovely Gupta, Jata Shankar, Pooja Vijayaraghavan   +5 more
doaj   +1 more source

Reduced Activity of Triazole Antifungal Agents Against Aspergillus tubingensis From a Global Collection of Genomically‐Characterized Aspergillus Section Nigri Clinical Isolates (2020–2023)

Mycoses, Volume 69, Issue 7, July 2026.
ABSTRACT Background The black moulds comprising Aspergillus section Nigri are clinically important organisms causing aspergillosis and otomycosis; triazole (azole) antifungal agents represent the primary prophylaxis and treatment option for these infections.
John H. Kimbrough, Marisa L. Winkler, Maura H. Karr, Jessica M. Ewald, Mariana Castanheira   +4 more
wiley   +1 more source

Detection of Substitutions at 98th, 121st, and 289th Amino Acid Residues in Cyp51A using Cycling Probes

Medical Mycology Journal, 2020
Azole-resistant Aspergillus fumigatus containing unique mutation(s) of cyp51A with tandem repeats in the promoter region has emerged and has become dispersed in environments worldwide. For this study, we designed primers and cycling probes to detect mutations associated with tandem repeats. Substitutions at the 293rd nucleotide (leucine or histidine at
Toyotome, Takahito, Arai, Teppei, Watanabe, Akira, Kamei, Katsuhiko   +3 more
openaire   +3 more sources

Pharmacophore-Based Virtual Screening of Novel Inhibitors and Docking Analysis for CYP51A from Penicillium italicum

Marine Drugs, 2017
Sterol 14α-demethylases from Cytochrome P450 family (CYP51s) are essential enzymes in sterol biosynthesis and well-known as the target of antifungal drugs. The 3D structure of CYP51A from Penicillium italicum (PiCYP51A) was constructed through homology modeling based on the crystal structure of human CYP51A (PDB: 3LD6).
Yuan, Yongze, Han, Rui, Cao, Qianwen, Yu, Jinhui, Mao, Jiali, Zhang, Tingfu, Wang, Shengqiang, Niu, Yuhui, Liu, Deli   +8 more
openaire   +3 more sources

Evaluation of mRNA Expression Levels of cyp51A and mdr1, Candidate Genes for Voriconazole Resistance in Aspergillus flavus

Jundishapur Journal of Microbiology, 2015
Voriconazole Resistance (VRC-R) in Aspergillus flavus isolates impacts the management of aspergillosis, since azoles are the first choice for prophylaxis and therapy. However, to the best of our knowledge, the mechanisms underlying voriconazole resistance are poorly understood.The present study was designed to evaluate mRNA expression levels of cyp51A ...
Fattahi, Azam, Zaini, Farideh, Kordbacheh, Parivash, Rezaie, Sasan, Safara, Mahin, Fateh, Roohollah, Farahyar, Shirin, Kanani, Ali, Heidari, Mansour   +8 more
openaire   +3 more sources

Comparison of Two Molecular Assays for Detection and Characterization of Aspergillus fumigatus Triazole Resistance and Cyp51A Mutations in Clinical Isolates and Primary Clinical Samples of Immunocompromised Patients

Frontiers in Microbiology, 2018
In hematological patients, the incidence of invasive aspergillosis (IA) caused by azole resistant Aspergillus fumigatus (ARAf) is rising. As the diagnosis of IA is rarely based on positive culture in this group of patients, molecular detection of ...
Patricia Postina, Julian Skladny, Tobias Boch, Oliver A. Cornely, Oliver A. Cornely, Axel Hamprecht, Peter-Michael Rath, Jörg Steinmann, Oliver Bader, Thomas Miethke, Anne Dietz, Natalia Merker, Wolf-Karsten Hofmann, Dieter Buchheidt, Birgit Spiess   +14 more
doaj   +1 more source

First Report of Human Infection Caused by Aspergillus steynii and Analysis of Its Whole‐Genome Characteristics

Transboundary and Emerging Diseases, Volume 2026, Issue 1, 2026.
The incidence of infections caused by rare pathogens has increased in recent years. This necessitates the development of effective diagnostic and therapeutic approaches. In this study, we report the first documented case of Aspergillus steynii‐induced pulmonary infection in humans.
Ruixuan Wang, Jingjing Chen, Na Wu, Min Yang, Ruiying Quan, Nan Xia, Hailong Li, Hua Nian, Ning Jiang, Yunzhuo Chu, Shitong Cheng, Sufei Tian, Nan-hua Chen   +12 more
wiley   +1 more source

Genotype–phenotype complexity of the TR46/Y121F/T289A cyp51A azole resistance mechanism in Aspergillus fumigatus

Fungal Genetics and Biology, 2015
The Aspergillus fumigatus cyp51A gene TR46/Y121F/T289A mutation is a new emerging resistance mechanism with high-level voriconazole (VOR) resistance, and elevated MICs to all other medical azoles. This is highly worrisome as VOR is the primary drug for the treatment of many aspergillus diseases.
Eveline Snelders, Simone M.T. Camps, Anna Karawajczyk, Antonius J.M.M. Rijs, Jan Zoll, Paul E. Verweij, Willem J.G. Melchers   +6 more
openaire   +4 more sources