Molecular Characterization of the Cytidine Monophosphate-N-Acetylneuraminic Acid Hydroxylase (CMAH) Gene Associated with the Feline AB Blood Group System. [PDF]
PLoS ONE, 2016 Cat's AB blood group system (blood types A, B, and AB) is of major importance in feline transfusion medicine. Type A and type B antigens are Neu5Gc and Neu5Ac, respectively, and the enzyme CMAH participating in the synthesis of Neu5Gc from Neu5Ac is ...
Toshinori Omi +18 more
doaj +6 more sources
Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase and Solute Carrier Family 35 Member A1 Are Required for Reovirus Binding and Infection. [PDF]
J Virol, 2020 Attachment factors and receptors are important determinants of dissemination and tropism during reovirus-induced disease. In a CRISPR cell survival screen, we discovered two genes, Cmas and Slc35a1 , which encode proteins required for sialic acid ...
Urbanek K +8 more
europepmc +7 more sources
Cytidine monophosphate N-acetylneuraminic acid synthetase enhances invasion of human triple-negative breast cancer cells. [PDF]
Onco Targets Ther, 2018 Cancer cells have altered bioenergetics, which contributes to their ability to proliferate, survive in unusual microenvironments, and invade other tissues. Changes in glucose metabolism can have pleomorphic effects on tumor cells.To investigate potential mechanisms responsible for the increased malignancy associated with altered glucose metabolism, we ...
O'Day EM +4 more
europepmc +6 more sources
Production of α1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning. [PDF]
J Reprod Dev, 2017 Gene-knockout pigs hold great promise as a solution to the shortage of organs from donor animals for xenotransplantation. Several groups have generated gene-knockout pigs via clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) and somatic cell nuclear transfer (SCNT).
Gao H +11 more
europepmc +5 more sources
Covalent modification of lys19 in the ctp binding site of cytidine 5′‐monophosphate n‐acetylneuraminic acid synthetase [PDF]
Protein Science, 1999 AbstractPeriodate oxidized CTP (oCTP) was used to investigate the importance of lysine residues in the CTP binding site of the cytidine 5′‐monophosphate N‐acetylneuraminic acid (CMP‐NeuAc) synthetase (EC 2.7.7.43) from Haemophilus ducreyi. The reaction of oCTP with the enzyme follows pseudo‐first‐order saturation kinetics, giving a maximum rate of ...
Michael V. Tullius +2 more
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Hydroxylation of N-acetylneuraminic Acid Influences the in vivo Tropism of N-linked Sialic Acid-Binding Adeno-Associated Viruses AAV1, AAV5, and AAV6 [PDF]
Frontiers in Medicine, 2021 Adeno-associated virus (AAV) vectors are promising candidates for gene therapy. However, a number of recent preclinical large animal studies failed to translate into the clinic. This illustrates the formidable challenge of choosing the animal models that
Estrella Lopez-Gordo +6 more
doaj +3 more sources
Mouse liver cytidine‐5′‐monophosphate‐N‐acetylneuraminic acid hydroxylase [PDF]
European Journal of Biochemistry, 1992 In this paper, we present the results of an investigation into the catalytic properties of CMP‐Neu5Ac hydroxylase (Neu5Ac: N‐acetylneuraminic acid) in high‐speed supernatants of mouse liver. The enzyme was most active in Hepes/NaOH pH 7.4 and was markedly inhibited by relatively small increases in ionic strength, though the inhibition was abolished by ...
Lee Shaw +4 more
openalex +3 more sources
Regulation of colominic acid biosynthesis by temperature: Role of cytidine 5′‐monophosphate N‐acetylneuraminic acid synthetase [PDF]
FEBS Letters, 1989 Synthesis of colominic acid in Escherichia coli K‐235 is strictly regulated by temperature. Evidence for the role of cytidine 5′‐monophospho‐N‐acetylneuraminic acid (CMP‐Neu5Ac) synthetase in this regulation was obtained by measuring its level in E. coli grown at 20 and 37°C. No activity was found in E. coli grown at 20°C.
C González-Clemente +3 more
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Mammalian cytidine 5′-monophosphate N -acetylneuraminic acid synthetase: A nuclear protein with evolutionarily conserved structural motifs [PDF]
Proceedings of the National Academy of Sciences, 1998 Sialic acids of cell surface glycoproteins and glycolipids play a pivotal role in the structure and function of animal tissues. The pattern of cell surface sialylation is species- and tissue-specific, is highly regulated during embryonic development, and changes with stages of differentiation. A prerequisite for the synthesis of sialylated
Anja-K. Münster +5 more
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Identification of new participants in the rainbow trout (
Reproductive Biology and Endocrinology, 2006 Background The hormonal control of oocyte maturation and ovulation as well as the molecular mechanisms of nuclear maturation have been thoroughly studied in fish.
Nguyen Thaovi +3 more
doaj +2 more sources