Results 21 to 30 of about 1,917 (174)

The First Clinical Renal Xenotransplantation Study (EXPAND): Evaluating Safety, Function, and Zoonotic Risks. [PDF]

Health Care Sci
Health Care Science, Volume 4, Issue 6, Page 377-380, December 2025.
Nashan B.
europepmc   +2 more sources

Multiple gene editing in porcine embryos using a combination of microinjection, electroporation, and transfection methods [PDF]

Veterinary World, 2022
Background and Aim: Mosaicism – the presence of both wild-type and mutant alleles – is a serious problem for zygotic gene modification through gene editing using the Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR/ Cas9) system ...
Quynh Anh Le, Manita Wittayarat, Zhao Namula, Qingyi Lin, Koki Takebayashi, Maki Hirata, Fuminori Tanihara, Lanh Thi Kim Do, Takeshige Otoi   +8 more
doaj   +1 more source

N-Glycolylneuraminic Acid in Animal Models for Human Influenza A Virus

Viruses, 2021
The first step in influenza virus infection is the binding of hemagglutinin to sialic acid-containing glycans present on the cell surface. Over 50 different sialic acid modifications are known, of which N-acetylneuraminic acid (Neu5Ac) and N ...
Cindy M. Spruit, Nikoloz Nemanichvili, Masatoshi Okamatsu, Hiromu Takematsu, Geert-Jan Boons, Robert P. de Vries   +5 more
doaj   +1 more source

N-glycolylneuraminic acid as a carbohydrate cancer biomarker

Translational Oncology, 2023
One of the forms of aberrant glycosylation in human tumors is the expression of N-glycolylneuraminic acid (Neu5Gc). The only known enzyme to biosynthesize Neu5Gc in mammals, cytidine-5′-monophosphate-N-acetylneuraminic acid (CMAH), appears to be ...
Jing Wang, Lucy K. Shewell, Christopher J. Day, Michael P. Jennings   +3 more
doaj   +1 more source

Protein engineering of CMP kinases to improve thermal stability and resultant production of 3′-sialyllactose

Biotechnology & Biotechnological Equipment, 2022
Protein engineering is an effective method to improve the thermal stability of biological macromolecules. Cytidine monophosphate kinase (CMK) is a key enzyme involved in nucleotide metabolism and participates in the production of cytidine triphosphate ...
Jingjing Wang, Jinyong Wu, Zhongkui Li, Xiangsong Chen, Weiwei Liu, Jianming Yao   +5 more
doaj   +1 more source

Metabolomic Insights Into the Impact of a Maternal Western-Style Diet and Leptin Supplementation During Suckling on Adult Rat Offspring. [PDF]

FASEB J
Plasma metabolites in adult offspring reflecting metabolic programming during critical developmental windows. ABSTRACT Perinatal nutrition influences offspring metabolic phenotype and obesity risk. This study examines how a maternal obesogenic diet and offspring's leptin supplementation during suckling affect the metabolome in offspring adulthood ...
Pomar CA, Martin-Chamorro RA, Zouhar P, Castillo P, Cajka T, Kopecky J, Palou A, Palou M, Picó C.   +8 more
europepmc   +2 more sources

Triple gene editing in porcine embryos using electroporation alone or in combination with microinjection [PDF]

Veterinary World, 2022
Background and Aim: We previously developed the gene-editing by electroporation (EP) of Cas9 protein method, in which the CRISPR/Cas9 system was introduced into porcine in vitro fertilized (IVF) zygotes through EP to disrupt a target gene.
Zhao Namula, Quynh Anh Le, Manita Wittayarat, Qingyi Lin, Koki Takebayashi, Maki Hirata, Lanh Thi Kim Do, Fuminori Tanihara, Takeshige Otoi   +8 more
doaj   +1 more source

Tumor desialylation surpasses anti-PD-L1 checkpoint therapy in restoring anti-tumor immunity in a murine model for colorectal cancer. [PDF]

Int J Cancer
What's new? Our research highlights how tumor sialylation affects tumor growth and reshapes the immune tumor microenvironment in colorectal cancer. We demonstrate for the first time that complete removal of sialic acids from colorectal tumor cells elicits a robust anti‐tumor immune response, but does not enhance anti‐PD‐L1 blockade efficacy.
van der Haar Àvila I, Zhang T, Lorrain V, Keuning E, Ali LA, Chadick C, García-García S, Boon L, de Haan N, García-Vallejo JJ, van Kooyk Y, van Vliet SJ.   +11 more
europepmc   +2 more sources

Enhanced Bacterial α(2,6)-Sialyltransferase Reaction through an Inhibition of Its Inherent Sialidase Activity by Dephosphorylation of Cytidine-5'-Monophosphate. [PDF]

PLoS ONE, 2015
Bacterial α(2,6)-sialyltransferases (STs) from Photobacterium damsela, Photobacterium sp. JT-ISH-224, and P. leiognathi JT-SHIZ-145 were recombinantly expressed in Escherichia coli and their ST activities were compared directly using a galactosylated bi ...
Ji-Yeon Kang, Se-Jong Lim, Ohsuk Kwon, Seung-Goo Lee, Ha Hyung Kim, Doo-Byoung Oh   +5 more
doaj   +1 more source

Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells

Frontiers in Molecular Biosciences, 2022
N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5′-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is ...
Wenqian Yang, Yuan Jiang, Qulian Guo, Zhixin Tian, Zhigang Cheng   +4 more
doaj   +1 more source