Results 11 to 20 of about 62,191 (286)
Cytosine Methylation: Quantitation by Automated Genomic Sequencing and GENESCANTM Analysis
BioTechniques, 1996 Bisulfite treatment and PCR amplification of genomic DNA permits the methylation analysis of any cytosine residue in a target sequence. By cloning and sequencing the PCR product, the methylation of individual molecules can be determined, whereas direct ...
Cheryl L. Paul, Susan J. Clark
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Epigenetic switch reveals CRISPR/Cas9 response to cytosine methylation in plants. [PDF]
New Phytol, 2022 Commentary on Přibylová et al. (2022). 235 p 2285-2299 Genome editing techniques, such as the CRISPR/Cas9 system, offer a game-changing opportunity for crop improvement by enabling precise modifications to be made at targeted genomic loci.
Raffan S, Kaur N, Halford NG.
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Uncovering plant epigenetics: new insights into cytosine methylation in rye genomes. [PDF]
J Exp Bot, 2023 In plants, epigenetic modifications play an important role in the regulation of gene expression under different environmental conditions. Methylated cytosine (5mC) is a modification found in many eukaryotes, including plants.
Kaur N, Nayakoti S, Brock N, Halford NG.
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Dissecting cytosine methylation mechanics of dysmetabolism. [PDF]
Aging (Albany NY), 2019 Cencioni C, Gaetano C, Spallotta F.
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Mammalian cytosine methylation at a glance [PDF]
Journal of Cell Science, 2009 In mammals, methylation at symmetric CpG dinucleotides is necessary for the heritable silencing and regulation of many different classes of DNA sequences. These include highly abundant retrotransposons, such as the long terminal repeat (LTR)-based endogenous retroviruses, [e.g.
Steen K T, Ooi +2 more
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Cytosine Methylation: Remaining Faithful [PDF]
Current Biology, 2008 DNA methyltransferase-1 (DNMT1) has a higher specific activity on hemimethylated DNA than on unmethylated DNA, but this preference is too small to explain the faithful mitotic inheritance of genomic methylation patterns. New genetic studies in plants and mammals have identified a novel factor that increases the fidelity of maintenance methylation.
Ooi, Steen K.T., Bestor, Timothy H.
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Cytosine methylation and mammalian development [PDF]
Genes & Development, 1999 Programmed methylation and demethylation of regulatory sequences has been proposed to play a central role in vertebrate development. We report here that the methylation status of the 5′ regions of a panel of tissue-specific genes could not be correlated with expression in tissues of fetal and newborn mice.
C P, Walsh, T H, Bestor
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The methylation landscape of giga-genome and the epigenetic timer of age in Chinese pine
Nature Communications, 2023 DNA methylation level declines during aging of mammals. Here, the authors report single-base resolution landscape of cytosine DNA methylation at different ages of Chinese pine and show that the global cytosine DNA methylation gradually increases as age ...
Jiang Li +7 more
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Experimental and Computational Approaches for Non-CpG Methylation Analysis
Epigenomes, 2022 Cytosine methylation adjacent to adenine, thymine, and cytosine residues but not guanine of the DNA is distinctively known as non-CpG methylation. This CA/CT/CC methylation accounts for 15% of the total cytosine methylation and varies among different ...
Deepa Ramasamy +3 more
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Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. [PDF]
PLoS ONE, 2018 5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization ...
Aditi Singh +5 more
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