Results 31 to 40 of about 57,661 (242)

Genetic analysis of the cardiac methylome at single nucleotide resolution in a model of human cardiovascular disease.

PLoS Genetics, 2014
Epigenetic marks such as cytosine methylation are important determinants of cellular and whole-body phenotypes. However, the extent of, and reasons for inter-individual differences in cytosine methylation, and their association with phenotypic variation ...
Michelle D Johnson, Michael Mueller, Martyna Adamowicz-Brice, Melissa J Collins, Pascal Gellert, Klio Maratou, Prashant K Srivastava, Maxime Rotival, Shahena Butt, Laurence Game, Santosh S Atanur, Nicholas Silver, Penny J Norsworthy, Sarah R Langley, Enrico Petretto, Michal Pravenec, Timothy J Aitman   +16 more
doaj   +1 more source

5-Methylcytosine profiles in mouse transcriptomes suggest the randomness of m5C formation catalyzed by RNA methyltransferase

BMC Research Notes, 2022
Objective 5-Methylcytosine (m5C) is a type of chemical modification on the nucleotides and is widespread in both DNA and RNA. Although the DNA m5C has been extensively studied over the past years, the distribution and biological function of RNA m5C still
Junfeng Liu
doaj   +1 more source

AICDA drives epigenetic heterogeneity and accelerates germinal center-derived lymphomagenesis

Nature Communications, 2018
In diffuse large B-cell lymphoma (DLBCL) increased epigenetic heterogeneity in the form of cytosine methylation is known to link to a poor clinical outcome.
Matt Teater, Pilar M. Dominguez, David Redmond, Zhengming Chen, Daisuke Ennishi, David W. Scott, Luisa Cimmino, Paola Ghione, Jayanta Chaudhuri, Randy D. Gascoyne, Iannis Aifantis, Giorgio Inghirami, Olivier Elemento, Ari Melnick, Rita Shaknovich   +14 more
doaj   +1 more source

Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

BMC Plant Biology, 2019
Background De novo DNA methylation triggered by short interfering RNAs is called RNA-directed DNA methylation (RdDM). Transcriptional gene silencing (TGS) through RdDM can be induced using a viral vector.
Wataru Matsunaga, Hanako Shimura, Senri Shirakawa, Reika Isoda, Tsuyoshi Inukai, Takeshi Matsumura, Chikara Masuta   +6 more
doaj   +1 more source

Sodium Azide as a Chemical Mutagen in Wheat (Triticum aestivum L.): Patterns of the Genetic and Epigenetic Effects with iPBS and CRED-iPBS Techniques

Agriculture, 2023
Wheat, which is scientifically known as Triticum aestivum L., is a very nutritious grain that serves as a key component of the human diet. The use of mutation breeding as a tool for crop improvement is a reasonably rapid procedure, and it generates a ...
Aras Türkoğlu, Kamil Haliloğlu, Metin Tosun, Piotr Szulc, Fatih Demirel, Barış Eren, Henryk Bujak, Halit Karagöz, Marek Selwet, Güller Özkan, Gniewko Niedbała   +10 more
doaj   +1 more source

DNA methyltransferase 1, cytosine methylation, and cytosine hydroxymethylation in mammalian mitochondria [PDF]

Proceedings of the National Academy of Sciences, 2011
Mitochondrial DNA (mtDNA) has been reported to contain 5-methylcytosine (5mC) at CpG dinucleotides, as in the nuclear genome, but neither the mechanism generating mtDNA methylation nor its functional significance is known. We now report the presence of 5-hydroxymethylcytosine (5hmC) as well as 5mC in mammalian mtDNA, suggesting that previous studies ...
Lisa S, Shock, Prashant V, Thakkar, Erica J, Peterson, Richard G, Moran, Shirley M, Taylor   +4 more
openaire   +2 more sources

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

High-resolution analysis of cytosine methylation in ancient DNA. [PDF]

PLoS ONE, 2012
Epigenetic changes to gene expression can result in heritable phenotypic characteristics that are not encoded in the DNA itself, but rather by biochemical modifications to the DNA or associated chromatin proteins. Interposed between genes and environment,
Bastien Llamas, Michelle L Holland, Kefei Chen, Jennifer E Cropley, Alan Cooper, Catherine M Suter   +5 more
doaj   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

In planta assays involving epigenetically silenced genes reveal inhibition of cytosine methylation by genistein

Plant Methods, 2012
Background Cytosine methylation is involved in epigenetic control of gene expression in a wide range of organisms. An increasing number of examples indicate that changing the frequency of cytosine methylation in the genome is a feasible tool to engineer ...
Arase Sachiko, Kasai Megumi, Kanazawa Akira   +2 more
doaj   +1 more source