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Chromosome CPD(PI/DAPI)- and CMA/DAPI-Banding Patterns in Allium cepa L.
Russian Journal of Genetics, 2002Chromosome banding patterns of Allium cepa L. were obtained by using fluorochrome combinations chromomycin A3 (CMA) + 4',6-diamidino-2-phenylindole (DAPI), DAPI + actinomycin D (AMD) and propidium iodide (PI) + DAPI. In A. cepa, telomeric heterochromatin displayed dull fluorescence after staining with DAPI and DAPI/AMD.
E S, Kim, E O, Punina, A V, Rodionov
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DAPI staining improved for quantitative cytofluorometry
Histochemistry, 1983DNA-DAPI complexes emit strong bluish white fluorescence when excited by ultraviolet light so that even very small amounts of DNA such as those in mitochondria, chloroplasts, and virus particles can be visualized. Moreover, the staining procedure with DAPI is very simple and requires no hydrolysis.
S, Hamada, S, Fujita
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Applications of DAPI Cytochemistry to Neurobiology
Biotechnic & Histochemistry, 19924',6-Diamidino-2-phenylindole hydrochloride (DAPI) is a fluorescent dye with high affinity for DNA. We have employed it as a fluorescent chromatin counterstain on sections immunofluorescent-stained using rhodamine and on tissues enzymatically stained using beta-galactosidase.
P P, Sanna +3 more
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DAPI Staining of Drosophila Embryos
Cold Spring Harbor Protocols, 2007INTRODUCTIONDrosophila embryos can be stained with specific fluorescent probes or antibodies through either direct or indirect immunofluorescence. In particular, several effective probes exist for visualizing DNA. 4′,6-diamidino-2-phenylindole (DAPI) is a commonly used DNA-binding dye.
Wendy F, Rothwell, William, Sullivan
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Inositol phosphates induce DAPI fluorescence shift
Biochemical Journal, 2014The polymer inorganic polyP (polyphosphate) and inositol phosphates, such as IP6 (inositol hexakisphosphate; also known as phytic acid), share many biophysical features. These similarities must be attributed to the phosphate groups present in these molecules.
Bernadett, Kolozsvari +2 more
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Photochemistry and Photobiology, 1986
Abstract— The steady state and time resolved fluorescence of the drug and chromosomal staining agent, 4′,6‐diamidino‐2‐phenylindole dihydrochloride, DAPI, was examined under different solvent conditions. In solutions between pH 3 and pH 9 the fluorescence spectral maximum of DAPI was found at 460 nm.
Szabo, A. +4 more
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Abstract— The steady state and time resolved fluorescence of the drug and chromosomal staining agent, 4′,6‐diamidino‐2‐phenylindole dihydrochloride, DAPI, was examined under different solvent conditions. In solutions between pH 3 and pH 9 the fluorescence spectral maximum of DAPI was found at 460 nm.
Szabo, A. +4 more
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FISH Detection on DAPI-Banded Chromosomes
2003link_to_subscribed_fulltext
Heng, HH, Tsui, LC
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DOES DAPI DETECT CYTOPLASMIC DNA?
American Journal of Botany, 1995Ultrastructural observations have revealed that plastids are present in orchid pollen tubes, but the DNA‐binding fluorochrome 4',6‐diamidino‐2‐phenylindole (DAPI) does not localize any DNA in the pollen tube plastids at optimum binding and flourescence conditions.
Johansen, Bo Buchholt +3 more
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The Structure of DAPI Bound to DNA
Journal of Biomolecular Structure and Dynamics, 1989The structure of the DNA fluorochrome 4'-6-diamidine-2-phenyl indole (DAPI) bound to the synthetic B-DNA oligonucleotide C-G-C-G-A-A-T-T-C-G-C-G has been solved by single crystal x-ray diffraction methods, at a resolution of 2.4 A. The structure is nearly isomorphous with that of the native DNA molecule alone. With one DAPI and 25 waters per DNA double
T A, Larsen +4 more
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An Iodinated DAPI‐Based Reagent for Mass Cytometry
ChemBioChem, 2020AbstractMultiparametric single‐cell analysis has seen dramatic improvements with the introduction of mass cytometry (MC) and imaging mass cytometry (IMC™). These technologies expanded the number of biomarkers that can be identified simultaneously by using heavy‐isotope‐tagged antibody reagents.
Rahul Rana +5 more
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