Results 181 to 190 of about 6,008 (204)
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Biophysical and biochemical characterization of recombinant human Pop2 deadenylase
Protein Expression and Purification, 2008Pop2, a component of the Ccr4-Not complex, functions as a deadenylase both in vitro and in vivo. In this research, we found that the recombinant human Pop2 (hPop2) mainly existed in a compact monomeric state with a a + b tertiary structure type. The percentages of the secondary structures evaluated from the CD spectrum were about 37% a-helix, 14% b ...
Wei-Feng, Liu, Yong-Bin, Yan
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Activity and Function of Deadenylases
2012Shortening of the poly(A) tail is the first and often rate-limiting step in mRNA degradation. Three poly(A)-specific 3' exonucleases have been described that can carry out this reaction: PAN, composed of two subunits; PARN, a homodimer; and the CCR4-NOT complex, a heterooligomer that contains two catalytic subunits and may have additional functions in ...
Christiane, Harnisch +4 more
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PAN-orama: three convergent views of a eukaryotic deadenylase
Nature Structural & Molecular Biology, 2014Post-transcriptional mRNA regulation is often attained by lengthening or shortening the 3′ poly(A) tail of a transcript. Eukaryotic mRNAs show a spectrum of deadenylation rates, thus allowing intricate control of gene expression, but the mechanisms that determine such rates are unclear.
Sophie, Martin, Jeff, Coller
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2023
Deadenylase enzymes play a key role in mRNA degradation and RNA processing. In this chapter, we describe two activity assays for the quantitative biochemical analysis of deadenylase enzymes, which can easily be adapted for other nuclease enzymes. The assays use distinct principles of detection, which are based on differential annealing of a probe ...
Blessing, Airhihen +3 more
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Deadenylase enzymes play a key role in mRNA degradation and RNA processing. In this chapter, we describe two activity assays for the quantitative biochemical analysis of deadenylase enzymes, which can easily be adapted for other nuclease enzymes. The assays use distinct principles of detection, which are based on differential annealing of a probe ...
Blessing, Airhihen +3 more
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Diverse functions of deadenylases in DNA damage response and genomic integrity
WIREs RNA, 2020AbstractDNA damage response (DDR) is a coordinated network of diverse cellular processes including the detection, signaling, and repair of DNA lesions, the adjustment of metabolic network and cell fate determination. To deal with the unavoidable DNA damage caused by either endogenous or exogenous stresses, the cells need to reshape the gene expression ...
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Characterization of the ubiquitin binding properties of the deadenylase Pan2
The FASEB Journal, 2016Messenger RNA (mRNA) stability plays an important role in regulating gene expression in eukaryotic cells. While a number of endo‐ and exo‐nucleolytic enzymes act on mRNA, removal of the poly(A) tail, or deadenylation, is often the first and rate‐limiting step in mRNA decay.
Zachary Swinney +3 more
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Nocturnin is a Circadian-Regulated Deadenylase
2017A hallmark of circadian clock components is their rhythmic expression profile. Nocturnin, a rhythmically expressed gene, was first identified in the Xenopus laevis retina. Since then, nocturnin homologues have been identified in several other organisms, including mouse, where it is also expressed with a high amplitude circadian rhythmicity.
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The CCR4-NOT deadenylase activity contributes to generation of induced pluripotent stem cells
Biochemical and Biophysical Research Communications, 2016Somatic cells can be reprogrammed as induced pluripotent stem cells (iPSCs) by introduction of the transcription factors, OCT3/4, KLF4, SOX2, and c-MYC. The CCR4-NOT complex is the major deadenylase in eukaryotes. Its subunits Cnot1, Cnot2, and Cnot3 maintain pluripotency and self-renewal of mouse and human embryonic stem (ES) cells and contribute to ...
Ari Zukeran +6 more
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Novel interaction between CCR4 and CAF1 in rice CCR4–NOT deadenylase complex
Plant Molecular Biology, 2016Rice is an important crop in the world. However, little is known about rice mRNA deadenylation, which is an important regulation step of gene expression at the post-transcriptional level. The CCR4-NOT1 complex contains two key components, CCR4 and CAF1, which are the main cytoplasmic deadenylases in eukaryotic cells.
Wei-Lun, Chou +3 more
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Deadenylase-dependent mRNA decay of GDF15 and FGF21 orchestrates food intake and energy expenditure
Cell Metabolism, 2022Teppei Fujikawa +2 more
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