Results 11 to 20 of about 243 (115)
Analysis of recombinant yeast decapping enzyme [PDF]
RNA, 2003 A critical step in the turnover of yeast mRNAs is decapping. Two yeast proteins, Dcp1p and Dcp2p, are absolutely required for decapping, although their precise roles in the decapping reaction have not been established. To determine the function of both Dcp1p and Dcp2p in decapping, we purified recombinant versions of these proteins from Escherichia ...
Michelle, Steiger +4 more
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mBio, 2022 Decapping enzymes remove the 5′ cap of eukaryotic mRNA, leading to accelerated RNA decay. They are critical in regulating RNA homeostasis and play essential roles in many cellular and life processes.
Shuai Cao +4 more
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Poxvirus-encoded decapping enzymes promote selective translation of viral mRNAs.
PLoS Pathogens, 2020 Cellular decapping enzymes negatively regulate gene expression by removing the methylguanosine cap at the 5' end of eukaryotic mRNA, rendering mRNA susceptible to degradation and repressing mRNA translation. Vaccinia virus (VACV), the prototype poxvirus,
Fernando Cantu +5 more
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Is mRNA decapping by ApaH like phosphatases present in eukaryotes beyond the Kinetoplastida?
BMC Ecology and Evolution, 2021 Background ApaH like phosphatases (ALPHs) originate from the bacterial ApaH protein and have been identified in all eukaryotic super-groups. Only two of these proteins have been functionally characterised.
Paula Andrea Castañeda Londoño +3 more
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The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes. [PDF]
PLoS Pathogens, 2017 5'-3' decay is the major mRNA decay pathway in many eukaryotes, including trypanosomes. After deadenylation, mRNAs are decapped by the nudix hydrolase DCP2 of the decapping complex and finally degraded by the 5'-3' exoribonuclease. Uniquely, trypanosomes
Susanne Kramer
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Molecular Cell, 2012 In this issue of Molecular Cell, Brannan et al. (2012) propose a novel function for RNA-decapping and transcription termination in aborting "divergent" promoter-proximal elongation complexes.
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General decapping activators target different subsets of inefficiently translated mRNAs
eLife, 2018 The Dcp1-Dcp2 decapping enzyme and the decapping activators Pat1, Dhh1, and Lsm1 regulate mRNA decapping, but their mechanistic integration is unknown. We analyzed the gene expression consequences of deleting PAT1, LSM1, or DHH1, or the DCP2 C-terminal ...
Feng He +3 more
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Protected long-time storage of a topological insulator
AIP Advances, 2021 The physical investigation of surfaces and their properties crucially depends on their quality. Such investigations are commonly performed in an ultra-high vacuum environment.
Luo-Uei Liang +8 more
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Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis. [PDF]
PLoS Pathogens, 2015 Aberrant viral RNAs produced in infected plant cells serve as templates for the synthesis of dsRNAs. The derived virus-related small interfering RNAs (siRNA) mediate cleavage of viral RNAs by post-transcriptional gene silencing (PTGS), thus blocking ...
Jian Ye +8 more
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Control of mRNA decapping by autoinhibition [PDF]
Nucleic Acids Research, 2018 5’ mediated cytoplasmic RNA decay is a conserved cellular process in eukaryotes. While the functions of the structured core domains in this pathway are understood, the role of abundant intrinsically disordered regions (IDRs) is lacking. Here we reconstitute the Dcp1:Dcp2 complex containing a portion of the disordered C-terminus and show its activity is
Paquette, David R +3 more
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