Results 41 to 50 of about 243 (115)
End labeling of enzymatically decapped mRNA
Nucleic Acids Research, 1977 A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.
A, Efstratiadis +5 more
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Fast Decap Allocation Based on Algebraic Multigrid [PDF]
2006 IEEE/ACM International Conference on Computer Aided Design, 2006 Decap (decoupling capacitor) is an effective technique for suppressing power supply noise. Nevertheless, over-usage of decap usually causes excessive power dissipation. Therefore, the total decap area needs to be minimized subject to power supply noise constraints.
Cheng Zhuo +3 more
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HPat a decapping activator interacting with the miRNA effector complex.
PLoS ONE, 2013 Animal miRNAs commonly mediate mRNA degradation and/or translational repression by binding to their target mRNAs. Key factors for miRNA-mediated mRNA degradation are the components of the miRNA effector complex (AGO1 and GW182) and the general mRNA ...
Elisabeth Barišić-Jäger +4 more
doaj +1 more source
Wellcome Open Research, 2020 Background: Mechanisms of mRNA fate decisions play an important role in determining if a given mRNA will be translated, stored or degraded upon arrival to cytoplasm.
Nupur Bhatter +2 more
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Degradation of YRA1 Pre-mRNA in the cytoplasm requires translational repression, multiple modular intronic elements, Edc3p, and Mex67p. [PDF]
PLoS Biology, 2010 Intron-containing pre-mRNAs are normally retained and processed in the nucleus but are sometimes exported to the cytoplasm and degraded by the nonsense-mediated mRNA decay (NMD) pathway as a consequence of their inclusion of intronic in-frame termination
Shuyun Dong, Allan Jacobson, Feng He
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Transcriptome maps of general eukaryotic RNA degradation factors
eLife, 2019 RNA degradation pathways enable RNA processing, the regulation of RNA levels, and the surveillance of aberrant or poorly functional RNAs in cells. Here we provide transcriptome-wide RNA-binding profiles of 30 general RNA degradation factors in the yeast ...
Salma Sohrabi-Jahromi +7 more
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Nature Communications, 2018 The decapping enzyme Dcp2 removes the 5′ eukaryotic cap from mRNA transcripts and acts in concert with its essential activator Dcp1 and various coactivators.
Jeffrey S. Mugridge +4 more
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Rous Sarcoma Virus RNA Stability Element Inhibits Deadenylation of mRNAs with Long 3′UTRs
Viruses, 2017 All retroviruses use their full-length primary transcript as the major mRNA for Group-specific antigen (Gag) capsid proteins. This results in a long 3′ untranslated region (UTR) downstream of the termination codon. In the case of Rous sarcoma virus (RSV),
Vidya Balagopal, Karen L. Beemon
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Down-regulation of Decapping Protein 2 mediates chronic nicotine exposure-induced locomotor hyperactivity in Drosophila. [PDF]
PLoS ONE, 2012 Long-term tobacco use causes nicotine dependence via the regulation of a wide range of genes and is accompanied by various health problems. Studies in mammalian systems have revealed some key factors involved in the effects of nicotine, including ...
Jing Ren +6 more
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Biochemical and functional characterization of orf virus decapping protein OV71
Virology JournalBackground Nudix enzymes constitute a family of hydrolases that share a conserved Nudix motif, which catalyzes the hydrolysis of nucleoside diphosphates linked to another moiety X.
Mandanda N. Mthethwa +8 more
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