Results 51 to 60 of about 9,305 (205)

Translation of Enzymically Decapped Messenger RNA [PDF]

European Journal of Biochemistry, 1977
An enzymic procedure was used to remove the 7‐methylguanosine diphosphate moiety at the 5′ ends of rabbit hemoglobin mRNA and mouse immunoglobulin light‐chain mRNA. Evidence was obtained that the procedure, which involves the use of polynucleotide kinase, does not result in any further degradation of the mRNA.The enzymically decapped mRNA was as ...
K A, Abraham, A, Pihl
openaire   +2 more sources

Investigation of IRES Insertion into the Genome of Recombinant MVA as a Translation Enhancer in the Context of Transcript Decapping. [PDF]

PLoS ONE, 2015
Recombinant modified vaccinia virus Ankara (MVA) has been used to deliver vaccine candidate antigens against infectious diseases and cancer. MVA is a potent viral vector for inducing high magnitudes of antigen-specific CD8+ T cells; however the cellular ...
Naif Khalaf Alharbi, Senthil K Chinnakannan, Sarah C Gilbert, Simon J Draper   +3 more
doaj   +1 more source

The DEAD box helicase, Dhh1p, functions in mRNA decapping and interacts with both the decapping and deadenylase complexes [PDF]

RNA, 2001
A major pathway of mRNA turnover in eukaryotic cells initiates with deadenylation, leading to mRNA decapping and subsequent 5' to 3' exonuclease digestion. We show that a highly conserved member of the DEAD box family of helicases, Dhh1p, stimulates mRNA decapping in yeast. In dhh1delta mutants, mRNAs accumulate as deadenylated, capped species. Dhh1p's
J M, Coller, M, Tucker, U, Sheth, M A, Valencia-Sanchez, R, Parker   +4 more
openaire   +2 more sources

Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b

Life Science Alliance
The study represents the first functional dissection of the two human cofactors present in the RNA decapping complex, and, significantly, it evaluates their role in the transcript buffering system.
Ivana Vukovic, Samantha M Barnada, Jonathan W Ruffin, Jon Karlin, Ravi Kumar Lokareddy, Gino Cingolani, Steven B McMahon   +6 more
doaj   +1 more source

mRNA Decapping Factors LSM1 and PAT Paralogs Are Involved in Turnip Mosaic Virus Viral Infection

Molecular Plant-Microbe Interactions, 2022
Turnip mosaic virus is a devastating potyvirus infecting many economically important brassica crops. In response to this, the plant host engages its RNA silencing machinery, involving AGO proteins, as a prominent strategy to restrain turnip mosaic virus (
Zhangli Zuo, Milena Roux, Eleazar Rodriguez, Morten Petersen   +3 more
doaj   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System

Advanced Science, EarlyView.
Condensates at synapses organize synaptic vesicles (SVs) and are crucial for efficient neurotransmitter release, yet how RNA contributes to this mesoscale architecture remains poorly understood. Here we uncover that RNA modulates synapsin–driven condensates.
Branislava Rankovic, Zachary M. Geisterfer, Akshita Chhabra, Vladimir M. Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, Jingao Sun, Kiara Freitag, Katja Nowick, Marina Jendrach, Frank L. Heppner, Vladimir Despic, Michaela Müller‐McNicoll, Stephan Sigrist, Geert van den Bogaart, Sofiia Reshetniak, Amy S. Gladfelter, Dragomir Milovanovic   +21 more
wiley   +1 more source

Functional Link between the Mammalian Exosome and mRNA Decapping [PDF]

, 2001
Mechanistic understanding of mammalian mRNA turnover remains incomplete. We demonstrate that the 3′ to 5′ exoribonuclease decay pathway is a major contributor to mRNA decay both in cells and in cell extract.
Kiledjian, Megerditch, Wang, Zuoren
core   +1 more source

The eIF4E-binding protein Eap1p functions in Vts1p-mediated transcript decay. [PDF]

PLoS ONE, 2012
Sequence-specific RNA binding proteins can induce the degradation of mRNAs through their ability to recruit proteins that trigger transcript destabilization. For example, Vts1p, the S.
Laura M Rendl, Melissa A Bieman, Heli K Vari, Craig A Smibert   +3 more
doaj   +1 more source

In Vivo Direct Reprogramming: Current Progress and Future Prospects from Mechanisms to Therapeutic Application

Advanced Science, EarlyView.
This review examines the potential of in vivo direct reprogramming in regenerative medicine for functional tissue restoration, highlighting the role of tissue‐resident cues in generating functionally mature reprogrammed cells from lineage‐related cells. It contains a discussion on mechanisms, reprogramming factors, delivery approaches, and applications
Rishabh Deo Singh, Mauro Calvoli, Kyeong Kyu Kim   +2 more
wiley   +1 more source